RONALDO SOUSA OLIVEIRA FILHO

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LIM/35 - Laboratório de Nutrição e Cirurgia Metabólica do Aparelho Digestivo, Hospital das Clínicas, Faculdade de Medicina

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Revista / Livro: Journal of Parenteral and Enteral Nutrition ×

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  • article 2 Citação(ões) na Scopus
    Is There an Advantage in Enriching Parenteral Lipid Emulsions Containing Fatty Acids From Fish Oil With Medium-Chain Triglycerides? A Study on Body Pool Concentrations of omega-3 Fatty Acids in Lewis Rats
    (2021) TESSER, Alweyd; TORRINHAS, Raquel S. M. M.; GARLA, Priscila C.; OLIVEIRA-FILHO, Ronaldo S.; APROBATO, Felipe G. G.; TAMANAHA, Erika M.; ANTUNES, Marcia S.; SAMPAIO, Geni R.; TORRES, Elizabeth; CALDER, Philip C.; WAITZBERG, Dan Linetzky
    Background The addition of medium-chain triglycerides (MCTs) into parenteral lipid emulsions rich in fatty acids from fish oil (FOLEs) has been shown to improve their clearance and extrahepatic uptake. We assessed whether this effect could favor the leukocyte uptake of omega-3 polyunsaturated fatty acids (PUFAs) for immunomodulatory purposes Methods Following 5-day adaptation in metabolic cages, 42 male Lewis rats fed with AIN-93M chow were killed (baseline control group [BC]) or submitted to central venous catheterization and distributed into (1) surgical control group without parenteral infusion (chow group), (2) test emulsion (MCT/LCT/FO) group with the parenteral infusion of a FOLE containing 40% MCT, and (3) control emulsion group (LCT/FO) with the parenteral infusion of an FOLE without MCT. The 2 FOLEs had similar omega-3 PUFA contents and omega-6/omega-3 PUFA ratios and were infused during 48 and 72 hours. Concentrations of omega-3 and omega-6 PUFAs in plasma, liver, and blood mononuclear and polymorphonuclear leukocytes were assessed by gas chromatography Results In both FOLE groups, leukocyte concentrations of omega-3 PUFAs peaked after 48 hours' infusion (vs BC). At this time point, plasma concentrations of omega-3 PUFAs were higher in MCT/LCT/FO group than in LCT/FO group and the opposite was found in the liver (P<.05), but no differences in PUFA concentrations were observed between these groups in leukocytes (P>.05) Conclusion The omega-3 PUFAs provided by FOLEs rich in MCT were less incorporated by liver and remained more available for extrahepatic cell delivery, but this did not result in a clear benefit in increasing their incorporation by peripheral leukocytes.
  • article 1 Citação(ões) na Scopus
    Effect of a parenteral fish-oil-containing lipid emulsion on liver lipid perioxidation and antioxidative defenses in Lewis rats
    (2023) OLIVEIRA-FILHO, Ronaldo Sousa; TORRINHAS, Raquel Susana; TESSER, Alweyd; SAMPAIO, Geni R.; TORRES, Elizabeth; GARLA, Priscila Casarin; WAITZBERG, Dan Linetzky
    BackgroundEicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), naturally abundant in fish oil (FO), are known for their anti-inflammatory and potential antioxidant properties. The aim in this article is to evaluate the effect of the infusion of a parenteral FO-containing lipid emulsion on markers of liver lipid peroxidation and oxidative stress in rats undergoing central venous catheterization (CVC). MethodsAfter 5-day acclimatization, adult Lewis rats (n = 42) receiving a 20-g/day AIN-93M oral diet were randomly subdivided into four groups: (1) basal control (BC) (n = 6), without CVC or LE infusion; (2) SHAM (n = 12), with CVC but without LE infusion; (3) soybean oil (SO)/medium-chain triglyceride (MCT) (n = 12), with CVC and receiving LE without FO (4.3 g/kg fat); and (4) SO/MCT/FO (n = 12), with CVC and receiving LE containing 10% FO (4.3 g/kg fat). Animals from the BC group were euthanized immediately after acclimatization. The remaining groups of animals were euthanized after 48 or 72 h of surgical follow-up to assess profiles of liver and plasma fatty acids by gas chromatography, liver gene transcription factor Nrf2, F2-isoprostane lipid peroxidation biomarker, and the antioxidant enzymes glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) by enzyme-linked immunosorbent assay. R program (version 3.2.2) was utilized for data analysis. ResultsCompared with the other groups, liver EPA and DHA levels were higher in the SO/MCT/FO group, which also showed the highest liver Nrf2, GPx, SOD, and CAT levels and lower liver F2-isoprostane (P < 0.05). ConclusionExperimental delivery of FO via EPA and DHA sources in a parenteral LE was associated with a liver antioxidant effect.