MARCELO VIVOLO AUN
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/60 - Laboratório de Imunologia Clínica e Alergia, Hospital das Clínicas, Faculdade de Medicina
LIM/60 - Laboratório de Imunologia Clínica e Alergia, Hospital das Clínicas, Faculdade de Medicina
8 resultados
Resultados de Busca
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conferenceObject Bordetella Pertussis Whole-Cell Vaccine Inhibits Specific IgE, Inflammation and Airway Remodeling in a Murine Model of Asthma(2015) AUN, Marcelo Vivolo; ARANTES-COSTA, Fernanda; SARAIVA-ROMANHOLO, Beatriz Mangueira; ALMEIDA, Francine Maria; REGINA-BRUEGGERMANN, Thayse; MARTINS, Milton Arruda; KALIL, Jorge; GIAVINA-BIANCHI, PedroconferenceObject Sensitisation by subcutaneous route is superior to intraperitoneal route in the induction of asthma by house dust mite in a murine model(2013) V, M. Aun; SARAIVA-ROMANHOLO, B. M.; ARANTES-COSTA, F. M.; ALMEIDA, F. M.; MARTINS, M. A.; KALIL, J.; GIAVINA-BIANCHI, P.- Diphteria-tetanus-pertussis vaccine reduces specific IgE, inflammation and remodelling in an animal model of mite-induced respiratory allergy(2020) AUN, Marcelo Vivolo; ALMEIDA, Francine Maria de; SARAIVA-ROMANHOLO, Beatriz Mangueira; MARTINS, Milton de Arruda; KALIL, Jorge; ARANTES-COSTA, Fernanda Magalhaes; GIAVINA-BIANCHI, PedroBackground: Adjuvants, such as bacterial lipopolysaccharides, have been studied to improve the efficacy of allergen-specific immunotherapy. The Bordetella pertussis (Pw) vaccine has been shown to have a protective role in ovalbumin-induced asthma models. However, its role in allergy to mites is unknown. We evaluated the effects of the diphtheria-tetanus-pertussis (DTPw) vaccine on a murine model of respiratory allergy induced by Dermatophagoides pteronyssinus (Derp). Methods: In a 30-day protocol, BALB/c mice were immunized subcutaneously with saline or Derp, alone or in combination with diphtheria-tetanus (DT) or DTPw vaccines (days 0, 7 and 14). Subsequently, they underwent a daily intranasal challenge with saline or Derp (days 22-28) and were then sacrificed (day 29). We evaluated serum-specific immunoglobulins, bronchoalveolar lavage (BAL) cellularity, remodelling of the lower airways, density of polymorphonuclear leukocytes (PMNs) and acidic nasal mucus content. Results: The animals sensitized with Derp produced high levels of specific immunoglobulins, increased density of PMNs and nasal mucus content, and elevated BAL cellularity and remodelling. Vaccines led to a reduction in IgE levels, with the Derp-DTPw group being similar to the saline groups. The vaccinated groups had reductions of BAL cellularity and remodelling, with more expressive results in the Derp-DTPw group compared to the Derp-DT group. The DT and DTPw vaccines inhibited the nasal PMN infiltrate, and DTPw modulated the production of acidic nasal mucus. Conclusions: The DTPw vaccine reduced serum specific IgE, nasal and pulmonary inflammation and remodelling of the lower airways.
conferenceObject Inhibition of Inflammation and Mucus Production By Bordetella Pertussis Whole-Cell Vaccine in a Murine Model of Allergic Rhinitis(2016) AUN, Marcelo Vivolo; ARANTES-COSTA, Fernanda; ALMEIDA, Francine Maria; BRUGGERMANN, Thayse Regina; SARAIVA-ROMANHOLO, Beatriz Mangueira; GENARO, Isabella S.; MARTINS, Milton Arruda; KALIL, Jorge; GIAVINA-BIANCHI, Pedro- Clinical, functional and inflammatory evaluation in asthmatic patients after a simple short-term educational program: a randomized trial(2021) FELIX, Soraia Nogueira; AGONDI, Rosana Camara; AUN, Marcelo Vivolo; OLIVO, Clarice Rosa; ALMEIDA, Francine Maria de; AMORIM, Thais Santos; CEZARIO, Julia Caroline; GIAVINA-BIANCHI, Pedro; TIBERIO, Iolanda de Fatima Lopes Calvo; MARTINS, Milton de Arruda de; ROMANHOLO, Beatriz Mangueira SaraivaThis study aimed to evaluate the clinical evolution, functional parameters and inflammatory activity of asthma in patients who submitted to an educational intervention. 58 adult patients over 18 years of age with partly controlled and uncontrolled asthma were randomized into an intervention group (IG) (N = 32) and a control group (CG) (N = 26) and evaluated for 12 weeks. The Asthma Control Test (ACT), Asthma Control Questionnaire (ACQ), Asthma Quality Life Questionnaire (AQLQ) and Beck Depression Inventory (BDI) questionnaires were applied. Spirometry, exhaled nitric oxide (NO), exhaled breath condensate (EBC) and induced sputum (IS), measurement of the peak flow and symptoms were performed. The IG patients received an educational activity for 30 min applied by a nurse. Statistical analysis: analysis of variance with repeated intragroup measures. IG presented a decreased number of eosinophils in IS and IL-17A in EBC, an increase in the percentage of FEV1 before and after bronchodilator and an improvement in quality of life compared to the CG. There was an improvement in depression levels and a decrease in IL-4 and IL-5 in the IS and in the EBC in both groups. Our results suggest that an educational intervention can bring benefits concerning the control of inflammation, lung function alterations, quality of life and levels of depression in asthmatic patients. Registration: ClinicalTrials.gov; NCT03655392.
- Sensitization by subcutaneous route is superior to intraperitoneal route in induction of asthma by house dust mite in a murine mode(2015) AUN, Marcelo Vivolo; SARAIVA-ROMANHOLO, Beatriz Mangueira; ALMEIDA, Francine Maria de; BRüGGEMANN, Thayse Regina; KALIL, Jorge; MARTINS, Milton de Arruda; ARANTES-COSTA, Fernanda Magalhães; GIAVINA-BIANCHI, PedroABSTRACT Objective To develop a new experimental model of chronic allergic pulmonary disease induced by house dust mite, with marked production of specific immunoglobulin E (IgE), eosinophilic inflammatory infiltrate in the airways and remodeling, comparing two different routes of sensitization. Methods The protocol lasted 30 days. BALB/c mice were divided into six groups and were sensitized subcutaneously or intraperitoneally with saline (negative control), Dermatophagoides pteronyssinus (Der p) 50 or 500mcg in three injections. Subsequently they underwent intranasal challenge with Der p or saline for 7 days and were sacrificed 24 hours after the last challenge. We evaluated the titration of specific IgE anti-Der p, eosinophilic density in peribronchovascular space and airway remodeling. Results Both animals sensitized intraperitoneally and subcutaneously produced specific IgE anti-Der p. Peribronchovascular eosinophilia increased only in mice receiving lower doses of Der p. However, only the group sensitized with Der p 50mcg through subcutaneously route showed significant airway remodeling. Conclusion In this murine model of asthma, both pathways of sensitization led to the production of specific IgE and eosinophilia in the airways. However, only the subcutaneously route was able to induce remodeling. Furthermore, lower doses of Der p used in sensitization were better than higher ones, suggesting immune tolerance. Further studies are required to evaluate the efficacy of this model in the development of bronchial hyperresponsiveness, but it can already be replicated in experiments to create new therapeutic drugs or immunotherapeutic strategies.
- Regulatory T-Cell Distribution within Lung Compartments in COPD(2017) SALES, Davi S.; ITO, Juliana T.; ZANCHETTA, Ivy A.; ANNONI, Raquel; AUN, Marcelo V.; FERRAZ, Luiz Fernando S.; CERVILHA, Daniela A. B.; NEGRI, Elnara; MAUAD, Thais; MARTINS, Milton A.; LOPES, Fernanda D. T. Q. S.The importance of the adaptive immune response, specifically the role of regulatory T (Treg) cells in controlling the obstruction progression in smokers, has been highlighted. To quantify the adaptive immune cells in different lung compartments, we used lung tissues from 21 never-smokers without lung disease, 22 current and/or ex-smokers without lung disease (NOS) and 13 current and/or ex-smokers with chronic obstructive pulmonary disease (COPD) for histological analysis. We observed increased T, B, IL-17 and BAFF(+) cells in small and large airways of COPD individuals; however, in the NOS, we only observed increase in T and IL-17(+) cells only in small airways. A decrease in the density of Treg(+), TGF-beta(+) and IL-10(+) in small and large airways was observed only in COPD individuals. In the lymphoid tissues, Treg, T,B-cells and BAFF(+) cells were also increased in COPD; however, changes in Treg inhibitory associated cytokineswere not observed in this compartment. Therefore, our results suggest that difference in Treg(+) cell distributions in lung compartments and the decrease in TGF-beta(+) and IL-10(+) cells in the airways may lead to the obstruction in smokers.
conferenceObject Clinical, Functional and Inflammatory Evaluation in Asthmatic Patients After a Simple Short-Term Educational Program(2018) FELIX, S. N.; AGONDI, R. C.; AUN, M. V.; KURKEJAK, A.; AVONA, M. D.; AMORIM, T. S.; LOPES, M. R.; ALMEIDA, F. M.; GENARO, I. S.; BEZERRA, S. K.; GIAVINA-BIANCHI, P.; KALIL, J.; TIBERIO, I. D.; MARTINS, M. D.; SARAIVA-ROMANHOLO, B. M.