VERA LUCIA PAGLIUSI CASTILHO
Índice h a partir de 2011
9
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/03 - Laboratório de Medicina Laboratorial, Hospital das Clínicas, Faculdade de Medicina
LIM/03 - Laboratório de Medicina Laboratorial, Hospital das Clínicas, Faculdade de Medicina
10 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 10
conferenceObject POTENTIAL IMMUNOLOGICAL MARKERS FOR DIAGNOSIS OF HUMAN STRONGYLOIDIASIS USING HETEROLOGOUS ANTIGENS(2017) GRYSCHEK, Ronaldo; CORRAL, Marcelo; PAULA, Fabiana; MEISEL, Dirce; CASTILHO, Vera; GONCALVES, Elenice; LEVY, Debora; BYDLOWSKI, Sergio; CHIEFFI, Pedro Paulo; CASTRO-BORGES, William- Culture isolation and molecular identification of Blastocystis sp. in Brazilian human isolates: preliminary results(2020) MELO, Gessica Baptista de; ROLDAN, William; MALTA, Fernanda de Mello; LESCANO, Susana Angelica Zevallos; CASTILHO, Vera Lucia; GONCALVES, Elenice Messias Do Nascimento; PAULA, Fabiana Martins de; GRYSCHEK, Ronaldo Cesar BorgesBlastocystis sp. is a protist commonly found in stool samples of humans and animals. Biological and genetic factors of this organism remain controversial. The present study aimed to develop and implement the Blastocystis in vitro culture of Brazilian human isolates for routine use. The fecal isolates (n = 20) were maintained in our laboratory by several passages in Pavlova's medium. Cultures were monitored every 72 h by light microscopy. Genomic DNA was extracted to identify the subtypes (STs). In most isolates, the vacuolar form was prevalent. The amoeboid, granular and cystic forms were observed during in vitro cultivation. STs 1, 2, 3. 4 and 7 were identified. Our preliminary results show the generation time and forms present in the in vitro culture of Blastocystis subtypes isolated from Brazilian human isolates. Therefore, we emphasize the use of in vitro culture as a tool in future studies for the better understanding of the biological aspects of Blastocystis sp.
- DIAGNOSIS OF Strongyloides stercoralis INFECTION IN IMMUNOCOMPROMISED PATIENTS BY SEROLOGICAL AND MOLECULAR METHODS(2016) PAULA, Fabiana Martins de; MALTA, Fernanda Mello; CORRAL, Marcelo Andreetta; MARQUES, Priscilla Duarte; GOTTARDI, Maiara; MEISEL, Dirce Mary Correia Lima; YAMASHIRO, Juliana; PINHO, Joao Renato Rebello; CASTILHO, Vera Lucia Pagliusi; GONCALVES, Elenice Messias do Nascimento; GRYSCHEK, Ronaldo Cesar Borges; CHIEFFI, Pedro PauloStrongyloidiasis is a potentially serious infection in immunocompromised patients. Thus, the availability of sensitive and specific diagnostic methods is desirable, especially in the context of immunosuppressed patients in whom the diagnosis and treatment of strongyloidiasis is of utmost importance. In this study, serological and molecular tools were used to diagnose Strongyloides stercoralis infections in immunosuppressed patients. Serum and stool samples were obtained from 52 patients. Stool samples were first analyzed by Lutz, Rugai, and Agar plate culture methods, and then by a quantitative real time polymerase chain reaction (qPCR). Serum samples were evaluated by an enzyme-linked immunosorbent assay (ELISA) using a soluble (AS) or a membrane fractions antigen (AM) obtained from alkaline solutions of the filariform larvae of Strongyloides venezuelensis. Of the 52 immunosuppressed patients, three (5.8%) were positive for S. stercoralis by parasitological methods, compared to two patients (3.8%) and one patient (1.9%) who were detected by ELISA using the AS and the AM antigens, respectively. S. stercoralis DNA was amplified in seven (13.5%) stool samples by qPCR. These results suggest the utility of qPCR as an alternative diagnostic tool for the diagnosis of S. stercoralis infection in immunocompromised patients, considering the possible severity of this helminthiasis in this group of patients.
conferenceObject PHYLOGENETIC ANALYSIS OF BLASTOCYSTIS SPP. ISOLATES IN CLINICAL STOOL SAMPLES FROM BRAZIL(2017) GRYSCHEK, Ronaldo; MELO, Gessica; PAULA, Fabiana; MALTA, Fernanda; MARUTA, Celina; CRIADO, Paulo; MAGRI, Marcello; CASTILHO, Vera; GONCALVES, EleniceconferenceObject REVALENCE OF SCHISTOSOMA MANSONI INFECTION AND OTHER PARASITIC DISEASES IN PERIPHERAL AREAS OF BARRA MANSA, RIO DE JANEIRO, BRAZIL(2017) ESPIRITO-SANTO, Maria Cristina C.; CHIEFFI, Pedro Paulo; PAULA, Fabiana Martins de; CASTILHO, Vera Lucia Pagliusi; GONCALVES, Elenice Messias do Nascimento; ORBAN, Magali; PINHO, Joao Renato Rebello; LUNA, Expedito Jose de Albuquerque; GRYSCHEK, Ronaldo Cesar BorgesconferenceObject GENETIC DIVERSITY OF BLASTOCYSTIS SUBTYPES IN PATIENTS WITH CHRONIC URTICARIA(2017) PAULA, Fabiana M.; MELO, Gessica B.; MALTA, Fernanda M.; MARUTA, Celina W.; CRIADO, Paulo R.; CASTILHO, Vera Lucia P.; GONCALVES, Elenice M. N.; SANTO, Maria Cristina Espirito; GRYSCHEK, Ronaldo Cesar- IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES(2015) CORRAL, Marcelo Andreetta; PAULA, Fabiana Martins de; GOTTARDI, Maiara; MEISEL, Dirce Mary Correia Lima; CASTILHO, Vera Lucia Pagliusi; GONCALVES, Elenice Messias do Nascimento; CHIEFFI, Pedro Paulo; GRYSCHEK, Ronaldo Cesar BorgesThe aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from S. venezuelensis parasitic females were prepared in phosphate-buffered saline (SSF and SMF, respectively), Tris-HCl (TSF and TMF, respectively), and an alkaline buffer (ASF and AMF, respectively). Serum samples obtained from patients with strongyloidiasis or, other parasitic diseases, and healthy individuals were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions SSF, TSF, and ASF showed 85.0%, 75.0%, and 80.0% sensitivity and 93.1%, 93.1%, and 87.5% specificity, respectively. Membrane fractions SMF, TMF, and AMF showed 80.0%, 75.0%, and 85.0% sensitivity, and 95.8%, 90.3%, and 91.7% specificity, respectively. In conclusion, the present results suggest that the fractions obtained from parasitic females, especially the SSF and SMF, could be used as alternative antigen sources in the serodiagnosis of human strongyloidiasis.
- IgG reactivity with 40-35 kDa soluble and membrane antigen of Strongyloides venezuelensis in immunocompromised patients(2019) CORRAL, Marcelo Andreetta; PAULA, Fabiana Martins de; MEISEL, Dirce Mary C. L.; ABDALA, Edson; COSTA, Silvia Figueiredo; PIERROTTI, Ligia Camera; YAMASHIRO, Juliana; GONCALVES, Elenice M. do Nascimento; CASTILHO, Vera Lucia P.; CHIEFFI, Pedro Paulo; GRYSCHEK, Ronald Cesar B.Immunocompromised patients constitute a risk group for the development of severe clinical forms of human strongyloidiasis. The diagnosis of this infection is primarily performed by parasitological techniques, but with low sensitivity. Serological techniques appear as an alternative, especially with heterologous antigens use. The aim of this study was to perform the Western blot technique by using S. venezuelensis infective third stage larva (iL3) soluble (TS) and membrane (TM) saline antigens to reveal immunoreactive bands in immunocompromised patients with strongyloidiasis. Serum samples from 117 parasitologically well-characterized patients were divided into four groups: S. stercoralis positive and immunocompetent (S + IC); S. stercoralis positive and immunocompromised (S + IP); negative and immunocompetent (S-IC); negative and immunocompromised (S-IP). A 40-35 kDa band was recognized by 100% of patients in the S + IC group in both antigenic fractions, and by 62.5% and 50% in the S + IP group using the TS and TM fractions, respectively. A 29 kDa band was recognized by 86.3% and 72.7% (for TS and TM, respectively) of patients in the S + IC group, and only by 12.5% of patients in the S + IP group on the TM antigen. Regardless of the patients' immunological condition, the 40-35 kDa band from S. venezuelensis was detected more frequently and can be used as an important marker to the immunodiagnosis of human strongyloidiasis.
conferenceObject IDENTIFICATION OF BLASTOCYSTIS SPP. GENOTYPES IN PATIENTS AT SAO PAULO CITY, BRAZIL(2015) MELO, Gessica B.; PAULA, Fabiana M.; MALTA, Fernanda M.; MARUTA, Celina W.; CRIADO, Paulo R.; CASTILHO, Vera L.; GONCALVES, Elenice M.; GRYSCHEK, Ronaldo C.- Detection of Schistosoma mansoni Antibodies in a Low-Endemicity Area Using Indirect Immunofluorescence and Circumoval Precipitin Test(2014) ESPIRITO-SANTO, Maria Cristina Carvalho do; PINTO, Pedro Luiz; GARGIONI, Cybele; ALVARADO-MORA, Monica Viviana; CASTILHO, Vera Lucia Pagliusi; PINHO, Joao Ranato Rebello; LUNA, Expedito Jose de Albuquerque; GRYSCHEK, Ronaldo Cesar BorgesParasitological diagnostic methods for schistosomiasis lack sensitivity, especially in regions of low endemicity. The objective of this study was to determine the prevalence of Schistosoma mansoni infections by antibody detection using the indirect immunofluorescence assay (IFA-IgM) and circumoval precipitin test (COPT). Serum samples of 572 individuals were randomly selected. The IFA-IgM and COPT were used to detect anti-S. mansoni antibodies. Of the patients studied, 15.9% (N = 91) were IFA-IgM positive and 5.1% (N = 29) had COPT reactions (P < 0.001 by McNemar's test). Immunodiagnostic techniques showed higher infection prevalence than had been previously estimated. This study suggests that combined use of these diagnostic tools could be useful for the diagnosis of schistosomiasis in epidemiological studies in areas of low endemicity.