WALTER BELDA JUNIOR
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Dermatologia, Faculdade de Medicina - Docente
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/50 - Laboratório de Patologia das Moléstias Infecciosas, Hospital das Clínicas, Faculdade de Medicina
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/50 - Laboratório de Patologia das Moléstias Infecciosas, Hospital das Clínicas, Faculdade de Medicina
3 resultados
Resultados de Busca
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- Macrophage subtypes in recurrent nodular basal cell carcinoma after Mohs micrographic surgery(2017) PADOVEZE, Emerson H.; CHIACCHIO, Nilton Di; OCAMPO-GARZA, Jorge; CERNEA, Selma S.; BELDA, Walter; SOTTO, Mirian N.BackgroundThe macrophages associated with solid tumors are related to the progression or regression of tumors, depending on the differentiation in M1 or M2. M2 subtype promotes angiogenesis, remodeling, and tissue repair (tumor proliferation). In contrast, M1 produces toxic mediators and presents antigens, destroying microorganisms and tumor cells. The microenvironment of most aggressive forms of basal cell carcinoma (BCC) shows an increase in macrophages due to M2 phenotype compared to noninvasive forms. The treatment of nodular BCC by Mohs micrographic surgery (MMS) provides high cure rates, but relapses can occur. AimsTo compare the total population of macrophages and their subpopulations M1 and M2 in cases of recurrent and nonrecurrent nodular BCC after excision by MMS. Materials & MethodsHistological sections obtained from paraffin blocks of nine cases of recurrent nodular BCC after MMS and 18 cases of nonrecurrent nodular BCC operated by MMS were immunostained for iNOS, CD204, CD163, and CD68. The expression of these markers was analyzed by image analysis. ResultsNo significant differences were found between the groups in relation to the average percentage of M1 cells, M2 cells, and total cells. Discussion and ConclusionA relationship was not seen between tumor-associated macrophages (TAM) and tumor recurrence.
- MOLECULAR TYPING AND DETECTION OF MACROLIDE RESISTENCE IN TREPONEMA PALLIDUM DNA FROM PATIENTS WITH PRIMARY SYPHILIS IN SAO PAULO, BRAZIL(2017) SATO, Neuza Satomi; MORAIS, Fatima R. B.; POLISEL, Juliana De Oliveira; BELDA, Walter; FAGUNDES, Luiz Jorge
- Immunopathological characterization of human cutaneous leishmaniasis lesions caused by Leishmania (Viannia) spp. in Amazonian Brazil(2017) GOMES, Claudia Maria Castro; SOUSA, Maria Gloria Teixeira; MENEZES, Joyce Prieto Bezerra; BATISTA, Marliane Campos; LIMA, Ana Carolina Stocco; BELDA JR., Walter; BRADSHAW, Daniel; GAMA, Monica Elinor Alves; LAURENTI, Marcia Dalastra; SILVEIRA, Fernando Tobias; CORBETT, Carlos Eduardo PereiraAmerican cutaneous leishmaniasis (ACL) is a chronic infectious disease caused by different protozoan species of Leishmania, and it is endemic in both tropical and subtropical countries. Using immunohistochemistry, we investigate the density of CD68(+), lysozyme(+), CD1a(+), factor XIIIa(+), CD4(+), CD8(+), CD56(+), interferon (IFN)-gamma(+), and inducible NO synthase (iNOS(+)) cells. These cells were analyzed from 22 biopsy samples obtained from the lesions of ACL patients, whose infection was caused by Leishmania (Viannia) spp. Histopathological analysis showed dense mononuclear inflammatory infiltration in the dermis, which was composed of lymphocytes, macrophages, plasma cells, and discrete tissue parasitism. Granulomatous reactions were also present in the majority of cases. The density of the activated macrophages was higher than that of inactivated macrophages in the lesions. The density of Langerhans cells (CD1a(+)) was lower than that of dermal dendrocytes (factor XIIIa(+)). The density of CD8(+) T lymphocytes was higher than that of CD4(+) T lymphocytes. The cellular density of these immunological markers in relation to the species of Leishmania demonstrated that L. (Viannia) sp. lesions had higher IFN-gamma expression than that Leishmania (Viania) braziliensis lesions. The evaluation of these markers, according to disease progression, did not reveal any significant differences. L. (Viannia) sp. infection leads to a favorable immune response in the host, as predominantly represented by lysozyme(+), factor XIIIa(+), CD8(+) T cells, and the expression of (IFN)-gamma(+) at the lesion site.