RAFAELA ALKMIN DA COSTA
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/57 - Laboratório de Fisiologia Obstétrica, Hospital das Clínicas, Faculdade de Medicina
LIM/57 - Laboratório de Fisiologia Obstétrica, Hospital das Clínicas, Faculdade de Medicina
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conferenceObject Longitudinal serum levels of Placental Growth Factor and sFlt-1 throughout gestation in normotensive pregnancies and those complicated by hypertensive disorders(2013) COSTA, R. A.; HOSHIDA, M. S.; ALVES, E. A.; V, R. P. Francisco; ZUGAIB, M.Background: Preeclampsia (PE) prevalence is higher in women with chronic hypertension (CH). Predictive markers for PE in this group could be particularly important. We aimed to evaluate serum levels of PlGF and sFlt-1 throughout pregnancies complicated by isolated PE, CH and PE superimposed on CH (PE+CH) compared to normotensive (NT) pregnancies. Methods: Peripheral blood samples have been collected from NT and CH pregnancies at gestational ages of 20, 26, 32 and 36 weeks and stored to be assayed by ELISA. This study was approved by local Ethics Committee. To date, samples have been partially assayed. Results: Levels of sFlt-1 increased throughout pregnancy in all groups. Levels of PlGF increased until 32 weeks in pregnancies not complicated by PE (NT and CH) and until 26 weeks in those complicated by PE (PE and PE+HC), thereafter PlGF levels decreased in all groups. Conclusions: preliminary data, still too short for statistical testing.conferenceObject Risk Factors for Postpartum Glucose Intolerance in Patients with GDM Diagnosed by Abnormal Fasting Blood Glucose(2016) SOUZA, Ana Crla; COSTA, Rafaela A.; PAGANOTI, Cristiane F.; ZUGAIB, Marcelo; FRANCISCO, Rossana P. V.conferenceObject Effect of BPP-10c in angiogenic factor expression at the placental villous in preeclampsia(2013) HOSHIDA, M. S.; COSTA, R. A.; BEVILACQUA, E.; V, R. P. Francisco; ZUGAIB, M.Background: BPP-10c is the snake proline-rich oligopeptides, known as bradykinin-potentiating peptide found in Bothrops jararaca. This decapeptide leads to argininosuccinate synthetase activation, consequently sustaining increased nitric oxide production, a critical endogenous molecule to reduce the arterial blood pressure. Angiogenic factor is implicated in the pathogenesis of preeclampsia. We aimed to analyze the effects of BPP-10c in gene expression of angiogenic factor in human chorionic villi explant. Methods: Placentae were collected at delivery from normotensive (NP) and preeclamptic pregnancies (PE). Placental villous explant were cultivated by 24 h with BPP-10c. RNA was extracted and VEGF, PlGF, VEGFR1 and VEGFR2 expression were analyzed by RT-PCR. Results: Compared to expression from NP, PlGF was reduced in PE and VEGF and its receptor were increased in PE. Villous explants BPP-10c-treated from PE exhibited expression of PlGF higher than NP. Discussion: These preliminary results suggest that the BPP-10c could be related with increased angiogenic factor involved in pathophysiology of PE.