KEITY SOUZA SANTOS
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina - Docente
LIM/19 - Laboratório de Histocompatibilidade e Imunidade Celular, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/19 - Laboratório de Histocompatibilidade e Imunidade Celular, Hospital das Clínicas, Faculdade de Medicina - Líder
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conferenceObject Immune Responses Of CVID Patients To COVID-19 Vaccines(2023) MEDEIROS, Giuliana Xavier de; FERREIRA, Loisi de Carvalho Pereira; MAGAWA, Jhosiene Yukari; KURAMOTO, Andreia; SASAHARA, Greyce Luri; FERREIRA, Marcelo; BARROS, Myrthes Maragna Toledo; KALIL, Jorge; MARINHO, Ana Karolina Barreto Berselli; CUNHA-NETO, Edecio; SANTOS, Keity Souza; KOKRON, Cristina- Is age associated with the development of antibodies against botulinum toxin?(2013) AUN, M. V.; CHUNG, T. M.; SANTOS, K. S.; BATTISTELLA, L. M. R.; RIZZO, L. V.; KALIL, J.; GIAVINA-BIANCHI, P.
- Immunodominant antibody responses directed to SARS-CoV-2 hotspot mutation sites and risk of immune escape(2023) OLIVEIRA, Jamille Ramos; RUIZ, Cesar Manuel Remuzgo; MACHADO, Rafael Rahal Guaragna; MAGAWA, Jhosiene Yukari; DAHER, Isabela Pazotti; URBANSKI, Alysson Henrique; SCHMITZ, Gabriela Justamante Haendel; ARCURI, Helen Andrade; FERREIRA, Marcelo Alves; SASAHARA, Greyce Luri; MEDEIROS, Giuliana Xavier de; JR, Roberto Carlos Vieira Silva; DURIGON, Edison Luiz; BOSCARDIN, Silvia Beatriz; ROSA, Daniela Santoro; SCHECHTMAN, Deborah; NAKAYA, Helder. I. I.; CUNHA-NETO, Edecio; GADERMAIER, Gabriele; KALIL, Jorge; COELHO, Veronica; SANTOS, Keity SouzaIntroductionConsidering the likely need for the development of novel effective vaccines adapted to emerging relevant CoV-2 variants, the increasing knowledge of epitope recognition profile among convalescents and afterwards vaccinated with identification of immunodominant regions may provide important information. MethodsWe used an RBD peptide microarray to identify IgG and IgA binding regions in serum of 71 COVID-19 convalescents and 18 vaccinated individuals. ResultsWe found a set of immunodominant RBD antibody epitopes, each recognized by more than 30% of the tested cohort, that differ among the two different groups and are within conserved regions among betacoronavirus. Of those, only one peptide, P44 (S415-429), recognized by 68% of convalescents, presented IgG and IgA antibody reactivity that positively correlated with nAb titers, suggesting that this is a relevant RBD region and a potential target of IgG/IgA neutralizing activity. DiscussionThis peptide is localized within the area of contact with ACE-2 and harbors the mutation hotspot site K417 present in gamma (K417T), beta (K417N), and omicron (K417N) variants of concern. The epitope profile of vaccinated individuals differed from convalescents, with a more diverse repertoire of immunodominant peptides, recognized by more than 30% of the cohort. Noteworthy, immunodominant regions of recognition by vaccinated coincide with mutation sites at Omicron BA.1, an important variant emerging after massive vaccination. Together, our data show that immune pressure induced by dominant antibody responses may favor hotspot mutation sites and the selection of variants capable of evading humoral response.
- MUC22, HLA-A, and HLA-DOB variants and COVID-19 in resilient super-agers from Brazil(2022) CASTELLI, Erick C.; CASTRO, Mateus V. de; NASLAVSKY, Michel S.; SCLIAR, Marilia O.; SILVA, Nayane S. B.; PEREIRA, Raphaela N.; CIRIACO, Viviane A. O.; CASTRO, Camila F. B.; MENDES-JUNIOR, Celso T.; SILVEIRA, Etiele de S.; OLIVEIRA, Iuri M. de; ANTONIO, Eduardo C.; VIEIRA, Gustavo F.; MEYER, Diogo; NUNES, Kelly; MATOS, Larissa R. B.; SILVA, Monize V. R.; WANG, Jaqueline Y. T.; ESPOSITO, Joyce; CORIA, Vivian R.; MAGAWA, Jhosiene Y.; SANTOS, Keity S.; CUNHA-NETO, Edecio; KALIL, Jorge; BORTOLIN, Raul H.; HIRATA, Mario Hiroyuki; DELL'AQUILA, Luiz P.; RAZUK-FILHO, Alvaro; BATISTA-JUNIOR, Pedro B.; DUARTE-NETO, Amaro N.; DOLHNIKOFF, Marisa; SALDIVA, Paulo H. N.; PASSOS-BUENO, Maria Rita; ZATZ, MayanaBackgroundAlthough aging correlates with a worse prognosis for Covid-19, super elderly still unvaccinated individuals presenting mild or no symptoms have been reported worldwide. Most of the reported genetic variants responsible for increased disease susceptibility are associated with immune response, involving type I IFN immunity and modulation; HLA cluster genes; inflammasome activation; genes of interleukins; and chemokines receptors. On the other hand, little is known about the resistance mechanisms against SARS-CoV-2 infection. Here, we addressed polymorphisms in the MHC region associated with Covid-19 outcome in super elderly resilient patients as compared to younger patients with a severe outcome. MethodsSARS-CoV-2 infection was confirmed by RT-PCR test. Aiming to identify candidate genes associated with host resistance, we investigated 87 individuals older than 90 years who recovered from Covid-19 with mild symptoms or who remained asymptomatic following positive test for SARS-CoV-2 as compared to 55 individuals younger than 60 years who had a severe disease or died due to Covid-19, as well as to the general elderly population from the same city. Whole-exome sequencing and an in-depth analysis of the MHC region was performed. All samples were collected in early 2020 and before the local vaccination programs started. ResultsWe found that the resilient super elderly group displayed a higher frequency of some missense variants in the MUC22 gene (a member of the mucins' family) as one of the strongest signals in the MHC region as compared to the severe Covid-19 group and the general elderly control population. For example, the missense variant rs62399430 at MUC22 is two times more frequent among the resilient super elderly (p = 0.00002, OR = 2.24). ConclusionSince the pro-inflammatory basal state in the elderly may enhance the susceptibility to severe Covid-19, we hypothesized that MUC22 might play an important protective role against severe Covid-19, by reducing overactive immune responses in the senior population.
- The oldest unvaccinated Covid-19 survivors in South America(2022) V, Mateus de Castro; SILVA, Monize V. R.; NASLAVSKY, Michel S.; SCLIAR, Marilia O.; NUNES, Kelly; PASSOS-BUENO, Maria Rita; CASTELLI, Erick C.; MAGAWA, Jhosiene Y.; ADAMI, Flavia L.; MORETTI, Ana I. S.; OLIVEIRA, Vivian L. de; BOSCARDIN, Silvia B.; CUNHA-NETO, Edecio; KALIL, Jorge; JOUANGUY, Emmanuelle; BASTARD, Paul; CASANOVA, Jean-Laurent; QUINONES-VEGA, Mauricio; SOSA-ACOSTA, Patricia; GUEDES, Jessica S. de; ALMEIDA, Natalia P. de; NOGUEIRA, Fabio C. S.; DOMONT, Gilberto B.; SANTOS, Keity S.; ZATZ, MayanaBackground Although older adults are at a high risk of severe or critical Covid-19, there are many cases of unvaccinated centenarians who had a silent infection or recovered from mild or moderate Covid-19. We studied three Brazilian supercentenarians, older than 110 years, who survived Covid-19 in 2020 before being vaccinated. Results Despite their advanced age, humoral immune response analysis showed that these individuals displayed robust levels of IgG and neutralizing antibodies (NAbs) against SARS-CoV-2. Enrichment of plasma proteins and metabolites related to innate immune response and host defense was also observed. None presented autoantibodies (auto-Abs) to type I interferon (IFN). Furthermore, these supercentenarians do not carry rare variants in genes underlying the known inborn errors of immunity, including particular inborn errors of type I IFN. Conclusion These observations suggest that their Covid-19 resilience might be a combination of their genetic background and their innate and adaptive immunity.
- Reactions to Shrimp Including Severe Anaphylaxis in Mite- and Cockroach-Allergic Patients Who Have Never Eaten Shrimp: Clinical Significance of IgE Cross-Reactivity to Tropomyosins From Different Sources(2019) MARTINS, T. F.; MENDONCA, T. N.; MELO, J. M. L.; MORENO, A. S.; JANUARIO, Y. C.; DASILVA, L. L. P.; DIAS, M. M.; MEIRELES, P. R.; SANTOS, K. S.; YANG, A. C.; ARRUDA, L. K.
conferenceObject Microarray Analysis of IgE Reactivity in Patients with Asthma and/or Rhinitis Allergic to Cockroach and Mites: Clinical Application(2012) BARDINI, G. A.; MORENO, A. S.; SANTOS, A. C. A. F.; SANTOS, K. S.; ARRUDA, L.RATIONALE: Microarray technology may improve diagnosis of allergy by making it possible to identify disease-causing allergens. We sought to investigate the IgE reactivity profiles in a panel of patients allergic to cockroach and mites by microarray. METHODS: Fifty-one patients with asthma and/or rhinitis, aged 12 to 75years-old, were selected based on presence of positive skin tests to cockroach and mites. ISAC was carried out and IgEprofiles were analyzed. RESULTS: IgE reactivity to mite allergens Der p 1,Der p 2,Der f 1,Der f 2,Eur m 2 and Der p 10(mite tropomyosin) was 71%,75%,71%,75%,41% and 51%, respectively. IgE to Bla g 1, Bla g 2 and Bla g 4 was observed in 1 patient each (2%), and to Bla g 5 in two patients (4%). On the other hand, IgE to Bla g 7(cockroach tropomyosin) was found in 26/51 (51%) of the patients. Sensitization to tropomyosin from shrimp and Anysakis simplex was observed in 47% and 53% of the patients, respectively. IgE to cat, bee venom,sesame and grass pollen was foundin10%-20%of the patients; IgE to all other allergens was detected in less than 10% of the patients. No IgE reactivity was detected in 8/9 non-allergic control individuals; only one had detectable IgE to Ole e 2. CONCLUSIONS: Microarray analysis was helpful to define IgE reactivity profiles. More that 70% of our patients showed reactivity to the major group 1 and 2 allergens. The results confirmed that tropomyosin is a major pan-allergen among patients with asthma and/or rhinitis in Brazil.- Allergic reactions to manioc (Manihot esculenta Crantz): Identification of novel allergens with potential involvement in latex-fruit syndrome(2011) SANTOS, Keity Souza; GALVAO, Clovis Eduardo; GADERMAIER, Gabriele; RESENDE, Virginia Maria Ferreira; MARTINS, Carlo de Oliveira; MISUMI, Denise Shimbo; YANG, Ariana Campos; FERREIRA, Fatima; PALMA, Mario Sergio; KALIL, Jorge; CASTRO, Fabio Fernandes Morato
conferenceObject Cross-Reactivity Among Cereal Grains(2016) MENDONCA, Juliana Guimaraes; CASTRO, Roberta Almeida; CORDOVA, Pablo Torres; MEIRELES, Paula Rezende; FIGO, Daniele Danella; SANTOS, Keity Souza; KALIL, Jorge; CASTRO, Fabio Fernandes Morato; YANG, Ariana C.- B-cell linear epitopes mapping of antigen-5 allergen from Polybia paulista wasp venom(2015) SANTOS-PINTO, Jose Roberto Aparecido dos; SANTOS, Lucilene Delazari dos; ARCURI, Helen Andrade; MENEGASSO, Anally Ribeiro da Silva; PEGO, Paloma Napoleao; SANTOS, Keity Souza; CASTRO, Fabio Morato; KALIL, Jorge Elias; DE-SIMONE, Salvatore Giovanni; PALMA, Mario Sergio