THAIS MAUAD
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
16 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 16
- Influence of Air Pollution and Soil Contamination on the Contents of Polycyclic Aromatic Hydrocarbons (PAHs) in Vegetables Grown in Urban Gardens of Sao Paulo, Brazil(2017) AMATO-LOURENCO, Luis F.; SAIKI, Mitiko; SALDIVA, Paulo H. N.; MAUAD, ThaisUrban community gardens (UCGs) have become prevalent worldwide and play a significant role in strengthening the sustainability of urban food systems. Although UCGs provide multiple benefits to society, the extent to which vegetables grown in them are contaminated by chemical compounds derived from airborne fallout or soil contamination is unclear. We evaluated the influence of both air pollution and the contamination of garden soil beds on the contents of 16 PAHs in the edible tissues of spinach and collard green. The PAH contents were quantified using gas chromatography-mass spectrometry (GC-MS-EI). The concentrations of PAHs in both the vegetables grown in the vessels and in the soil of the UCGs were < 0.1 mu g kg(-1). The total concentrations of the 16 priority PAHs in the soil beds ranged from 132.94 to 410.50 mu g kg(-1). These levels were lower than those that have been reported from other urban areas. Principal Component Analysis resulted in two components indicating that traffic-derived emissions are the main sources of PAHs in the soil. The first component represented the compounds with higher molecular weights and had a moderate loading for the fine fraction of the soil (clay) (0.63). The second component showed a high loading for silt (0.97), including those PAHs with lower molecular weights. Our data show that spinach and collard greens do not accumulate significant PAH concentrations on their leaves over 45-day periods, whether they are raised in controlled soil or in local beds.
- Effectiveness of traffic-related elements in tree bark and pollen abortion rates for assessing air pollution exposure on respiratory mortality rates(2017) CARVALHO-OLIVEIRA, Regiani; AMATO-LOURENCO, Luis F.; MOREIRA, Tiana C. L.; SILVA, Douglas R. Rocha; VIEIRA, Bruna D.; MAUAD, Thais; SAIKI, Mitiko; SALDIVA, Paulo H. NascimentoThe majority of epidemiological studies correlate the cardiorespiratory effects of air pollution exposure by considering the concentrations of pollutants measured from conventional monitoring networks. The conventional air quality monitoring methods are expensive, and their data are insufficient for providing good spatial resolution. We hypothesized that bioassays using plants could effectively determine pollutant gradients, thus helping to assess the risks associated with air pollution exposure. The study regions were determined from different prevalent respiratory death distributions in the Sao Paulo municipality. Samples of tree flower buds were collected from twelve sites in four regional districts. The genotoxic effects caused by air pollution were tested through a pollen abortion bioassay. Elements derived from vehicular traffic that accumulated in tree barks were determined using energy-dispersive X-ray fluorescence spectrometry (EDXRF). Mortality data were collected from the mortality information program of Sao Paulo City. Principal component analysis (PCA) was applied to the concentrations of elements accumulated in tree barks. Pearson correlation and exponential regression were performed considering the elements, pollen abortion rates and mortality data. PCA identified five factors, of which four represented elements related to vehicular traffic. The elements Al, S, Fe, Mn, Cu, and Zn showed a strong correlation with mortality rates (R-2>0.87) and pollen abortion rates (R-2>0.82). These results demonstrate that tree barks and pollen abortion rates allow for correlations between vehicular traffic emissions and associated outcomes such as genotoxic effects and mortality data.
- Relationship between Nrf2-Keap1 system and cell death in BEAS-2B exposed to Diesel Exhaust Particles(2017) FRIAS, Daniela; NUNES, Raquel; MATSUDA, Monique; YOSHIZAKI, Kelly; CARVALHO-OLIVEIRA, Regiani; PEREIRA, Daniela; VASCONCELLOS, Perola; MAUAD, Thais; MACCHIONE, Mariangela
- Effects of Intrauterine Exposure to Concentrated Ambient Particles on Mice Offspring Sensitized with House Dust Mite(2017) BRITO, Jose; ARANTES-COSTA, Fernanda Magalhaes; GUIMARAES, Eliane Tigre; VIEIRA, Rodolfo de Paula; MACCHIONE, Mariangela; MAUAD, Thais
- Airway and parenchyma immune cells in influenza A(H1N1) pdm09 viral and non-viral diffuse alveolar damage(2017) BUTTIGNOL, Monique; PIRES-NETO, Ruy Camargo; SILVA, Renata Calciolari Rossi e; ALBINO, Marina Ballarin; DOLHNIKOFF, Marisa; MAUAD, ThaisBackground: Diffuse alveolar damage (DAD), which is the histological surrogate for acute respiratory distress syndrome (ARDS), has a multifactorial aetiology. Therefore it is possible that the immunopathology differs among the various presentations of DAD. The aim of this study is to compare lung immunopathology of viral (influenza A(H1N1) pdm09) to non-viral, extrapulmonary aetiologies in autopsy cases with DAD. Methods: The lung tissue of 44 patients, was divided in the H1N1 group (n = 15) characterized by severe pulmonary injury due to influenza A(H1N1) pdm09 infection; the ARDS group (n = 13), characterized by patients with DAD due to non-pulmonary causes; and the Control group (n = 16), consisting of patients with non-pulmonary causes of death. Immunohistochemistry and image analysis were used to quantify, in the parenchyma and small airways, several immune cell markers. Results: Both DAD groups had higher expression of neutrophils and macrophages in parenchyma and small airways. However, there was a higher expression of CD4+ and CD8+ T lymphocytes, CD83+ dendritic cells, granzyme A+ and natural killer + cell density in the lung parenchyma of the H1N1 group (p < 0.05). In the small airways, there was a lower cell density of tryptase + mast cells and dendritic + cells and an increase of IL-17 in both DAD groups (p < 0.05). Conclusion: DAD due to viral A(H1N1) pdm09 is associated with a cytotoxic inflammatory phenotype, with partially divergent responses in the parenchyma relative to the small airways. In non-viral DAD, main immune cell alterations were found at the small airway level, reinforcing the role of the small airways in the pathogenesis of the exudative phase of DAD.
- Diesel exhaust exposure and sirtuins gene expression in old mice(2017) RIBEIRO, Gabriel; COSTA, Natalia de Souza Xavier; BELOTTI, Luciano; ALEMANY, Adair; CUNHA, Paula Gabriela; DURO, Stephanie de Oliveira; MARCOURAKIS, Tania; FARSKY, Sandra; MAUAD, Thais
- The effects of particulate matter on inflammation of respiratory system: Differences between male and female(2017) YOSHIZAKI, Kelly; BRITO, Jose Mara; SILVA, Luiz Fernando; LINO-DOS-SANTOS-FRANCO, Adriana; FRIAS, Daniela Perroni; SILVA, Renata Calciolari Rossi e; AMATO-LOURENCO, Luis Fernando; SALDIVA, Paulo Hilario Nascimento; TIBERIO, Iolanda de Fatima Lopes Calvo; MAUAD, Thais; MACCHIONE, MariangelaAir pollution is known to exacerbate respiratory diseases and epidemiological studies have shown that women present more chronic respiratory symptoms than man exposed to traffic pollution, however, the reason why is unclear. This study evaluated the inflammatory differences in BALB/c mouse males (n = 34) and females (n = 111) in three phases of the estrous cycle that were exposed to ambient air (AA) or concentrated ambient particles (CAPs). Tracheal hyperreactivity to methacholine, bronchoalveolar lavage fluid (BALF) and immunohistochemical of airways and lung parenchyma were studied. Hyperreactivity increased in CAPs-exposed female mice compared with AA-exposed mice in estrus (p < 0.05) and proestrus phases (p < 0.05) and decreased in CAPs-exposed males compared with those exposed to AA (p < 0.05). Males had increased numbers of total cells (p = 0.037) and macrophages (p = 0.028) compared to females. BALF levels of cyclooxygenase-2(COX-2) (p = 0.000), transforming growth factor alpha (TGF-alpha) (p = 0.001) and IL-8 receptor alpha (IL-8R alpha) (p = 0.014) were increased in males compared with proestrus, estrus and diestrus females, independent of exposure. Proestrus females exhibited significantly higher cadherin expression in lung parenchyma than did males (p = 0.005). CAPs exposure increased matrix metalloproteinase-9 (MMP-9) (p = 0.024) and isoprostane (p = 0.003) expression in the airways of both, males and females. The level of substance P (SP) (p = 0.001) increased in lung parenchyma in males compared with females, while IL-17 levels in airways (p = 0.042) and in lung parenchyma (p = 0.008) increased in females. MMP-9 levels (p = 0.024) were significantly lower in the lung parenchyma of CAPs-exposed females. TGF-alpha (p = 0.007) levels increased in the lung parenchyma of CAPs-exposed females compared to AA-exposed females. These results suggest that inflammatory markers differentially expressed in male mice were mostly linked to acute inflammation (IL-1 beta, IL-8R alpha, COX-2), whereas in females, markers that may lead to a chronic inflammatory process such as IL-17 and remodeling (MMP-9) were increased.
- Mast cell subtypes in adults and children with severe asthma(2017) ARAUJO-PAULINO, Bianca Bergamo de; ELLER, Miriam Cardoso Neves; VERGANI, Karina Pierantozzi; CARVALHO-PINTO, Regina Maria; STELMACH, Rafael; YOSHIZAKI, Kelly; GROTH, Espen Elias; RODRIGUES, Joaquim; DOLHNIKOFF, Marisa; MAUAD, Thais
- Regulatory T cells in COPD development: How the animal model resembles the human pathophysiological features(2017) ITO, Juliana Tiyaki; CERVILHA, Daniela Aparecida de Brito; SILVA, Larissa Emidio de Franca; LOURENCO, Juliana Dias; MOREIRA, Alyne Riani; KOHLER, Julia Benini; NEGRI, Elnara Marcia; MACCHIONE, Mariangela; MAUAD, Thais; MARTINS, Milton Arruda; LOPES, Fernanda Degobbi Tenorio Quirino dos Santos
- Identification of IL-17F/frequent exacerbator endotype in asthma(2017) RICCIARDOLO, Fabio L. M.; SORBELLO, Valentina; FOLINO, Anna; GALLO, Fabio; MASSAGLIA, Gian Mario; FAVATA, Gabriella; CONTICELLO, Salvatore; VALLESE, Davide; GANI, Federica; MALERBA, Mario; FOLKERTS, Gert; ROLLA, Giovanni; PROFITA, Mirella; MAUAD, Thais; STEFANO, Antonino Di; CIPRANDI, GiorgioBackground: Severe asthma might be associated with overexpression of T(H)17 cytokines, which induce neutrophil recruitment via neutrophil-mobilizing cytokines in airways. Objective: To study IL-17-related cytokines in nasal/bronchial biopsies from controls and mild asthmatics (MAs) to severe asthmatics (SAs) in relation to exacerbation rate. Methods: Inflammatory cells and IL-17A(+), IL-17F(+), IL-21(+), IL-22(+), and IL-23(+) cells were examined by immunohistochemistry in cryostat sections of bronchial/nasal biopsies obtained from 33 SAs (21 frequent exacerbators [FEs]), 31 MAs (3 FEs), and 14 controls. IL-17F protein was also measured by ELISA in bronchial/nasal lysates and by immunohistochemistry in bronchial tissue obtained from subjects who died because of fatal asthma. Immunofluorescence/confocal microscopy was used for IL-17F colocalization. Results: Higher number (P < .05) of neutrophils, IL-17A(+), IL-17F(+), and IL-21(+) cells in bronchial biopsies and higher numbers (P < .01) of IL-17F(+) and IL-21(+) cells in nasal biopsies were observed in SAs compared with MAs. Bronchial IL-17F(+) cells correlated with bronchial neutrophils (r = 0.54), exacerbation rate (r = 0.41), and FEV1 (r 5 20.46). Nasal IL-17F(+) cells correlated with bronchial IL-17F (r = 0.35), exacerbation rate (r = 0.47), and FEV1 (r520.61). FEs showed increased number of bronchial neutrophils/eosinophils/CD4(+)/CD8(+) cells and bronchial/nasal IL-17F(1) cells. Receiver operating characteristic curve analysis evidenced predictive cutoff values of bronchial neutrophils and nasal/bronchial IL-17F for discriminating between asthmatics and controls, between MAs and SAs and between FEs and non-FEs. IL-17F protein increased in bronchial/nasal lysates of SAs and FEs and in bronchial tissue of fatal asthma. IL-17F colocalized in CD4(+) /CD8(+) cells. Conclusions: IL-17-related cytokines expression was amplified in bronchial/nasal mucosa of neutrophilic asthma prone to exacerbation, suggesting a pathogenic role of IL-17F in FEs.