BIANCA HELENA VENTURA FERNANDES

(Fonte: Lattes)
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Lattes
Projetos de Pesquisa
Unidades Organizacionais
SCLAB-05, Faculdade de Medicina
LIM/42 - Laboratório de Hormônios e Genética Molecular, Hospital das Clínicas, Faculdade de Medicina

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Assunto: Chemistry, Multidisciplinary ×

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  • article 1 Citação(ões) na Scopus
    Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model
    (2023) ROSA, Ivana F.; PECANHA, Ana P. B.; CARVALHO, Tabata R. B.; ALEXANDRE, Leonardo S.; FERREIRA, Vinicius G.; DORETTO, Lucas B.; SOUZA, Beatriz M.; NAKAJIMA, Rafael T.; SILVA, Patrick da; BARBOSA, Ana P.; GOMES-DE-PONTES, Leticia; BOMFIM, Camila G.; MACHADO-SANTELLI, Glaucia M.; CONDINO-NETO, Antonio; GUZZO, Cristiane R.; PERON, Jean P. S.; ANDRADE-SILVA, Magaiver; CAMARA, Niels O. S.; GARNIQUE, Anali M. B.; MEDEIROS, Renata J.; FERRARIS, Fausto K.; BARCELLOS, Leonardo J. G.; CORREIA-JUNIOR, Jose D.; GALINDO-VILLEGAS, Jorge; MACHADO, Monica F. R.; CASTOLDI, Angela; OLIVEIRA, Susana L.; COSTA, Camila C.; BELO, Marco A. A.; GALDINO, Giovane; SGRO, German G.; BUENO, Natalia F.; ETO, Silas F.; VERAS, Flavio P.; FERNANDES, Bianca H. V.; SANCHES, Paulo R. S.; CILLI, Eduardo M.; MALAFAIA, Guilherme; NOBREGA, Rafael H.; GARCEZ, Aguinaldo S.; CARRILHO, Emanuel; CHARLIE-SILVA, Ives
    Although the exact mechanism of the pathogenesis of coronavirus SARS-CoV-2 (COVID-19) is not fully understood, oxidative stress and the release of pro-inflammatory cytokines have been highlighted as playing a vital role in the pathogenesis of the disease. In this sense, alternative treatments are needed to reduce the level of inflammation caused by COVID-19. Therefore, this study aimed to investigate the potential effect of red photobiomodulation (PBM) as an attractive therapy to downregulate the cytokine storm caused by COVID-19 in a zebrafish model. RT-qPCR analyses and protein-protein interaction prediction among SARS-CoV-2 and Danio rerio proteins showed that recombinant Spike protein (rSpike) was responsible for generating systemic inflammatory processes with significantly increased levels of pro-inflammatory (il1b, il6, tnfa, and nfkbiab), oxidative stress (romo1) and energy metabolism (slc2a1a and coa1) mRNA markers, with a pattern similar to those observed in COVID-19 cases in humans. On the other hand, PBM treatment was able to decrease the mRNA levels of these pro-inflammatory and oxidative stress markers compared with rSpike in various tissues, promoting an anti-inflammatory response. Conversely, PBM promotes cellular and tissue repair of injured tissues and significantly increases the survival rate of rSpike-inoculated individuals. Additionally, metabolomics analysis showed that the most-impacted metabolic pathways between PBM and the rSpike treated groups were related to steroid metabolism, immune system, and lipid metabolism. Together, our findings suggest that the inflammatory process is an incisive feature of COVID-19 and red PBM can be used as a novel therapeutic agent for COVID-19 by regulating the inflammatory response. Nevertheless, the need for more clinical trials remains, and there is a significant gap to overcome before clinical trials can commence.
  • article 19 Citação(ões) na Scopus
    Modular Label-Free Electrochemical Biosensor Loading Nature-Inspired Peptide toward the Widespread Use of COVID-19 Antibody Tests
    (2022) CASTRO, Ana C. H.; BEZERRA, Italo R. S.; PASCON, Aline M.; SILVA, Gabriela H. da; PHILOT, Eric A.; OLIVEIRA, Vivian L. de; MANCINI, Rodrigo S. N.; SCHLEDER, Gabriel R.; CASTRO, Carlos E.; CARVALHO, Luciani R. S. de; FERNANDES, Bianca H. V.; CILLI, Eduardo M.; SANCHES, Paulo R. S.; SANTHIAGO, Murilo; CHARLIE-SILVA, Ives; MARTINEZ, Diego S. T.; SCOTT, Ana L.; ALVES, Wendel A.; LIMA, Renato S.
    Limitations of the recognition elements in terms of synthesis, cost, availability, and stability have impaired the translation of biosensors into practical use. Inspired by nature to mimic the molecular recognition of the anti-SARS-CoV-2 S protein antibody (AbS) by the S protein binding site, we synthesized the peptide sequence of Asn-Asn-Ala-Thr-Asn-COOH (abbreviated as PEP2003) to create COVID-19 screen-ing label-free (LF) biosensors based on a carbon electrode, gold nanoparticles (AuNPs), and electrochemical impedance spec-troscopy. The PEP2003 is easily obtained by chemical synthesis, and it can be adsorbed on electrodes while maintaining its ability for AbS recognition, further leading to a sensitivity 3.4-fold higher than the full-length S protein, which is in agreement with the increase in the target-to-receptor size ratio. Peptide-loaded LF devices based on noncovalent immobilization were developed by affording fast and simple analyses, along with a modular functionalization. From studies by molecular docking, the peptide-AbS binding was found to be driven by hydrogen bonds and hydrophobic interactions. Moreover, the peptide is not amenable to denaturation, thus addressing the trade-off between scalability, cost, and robustness. The biosensor preserves 95.1% of the initial signal for 20 days when stored dry at 4 degrees C. With the aid of two simple equations fitted by machine learning (ML), the method was able to make the COVID-19 screening of 39 biological samples into healthy and infected groups with 100.0% accuracy. By taking advantage of peptide-related merits combined with advances in surface chemistry and ML-aided accuracy, this platform is promising to bring COVID-19 biosensors into mainstream use toward straightforward, fast, and accurate analyses at the point of care, with social and economic impacts being achieved.