REGIANI CARVALHO DE OLIVEIRA
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
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- Changes over time in the prevalence of asthma, rhinitis and atopic eczema in adolescents from Taubate, Sao Paulo, Brazil (2005-2012): Relationship with living near a heavily travelled highway(2016) TOTEDO, M. F.; SARAIVA-ROMANHOLO, B. M.; OLIVEIRA, R. C.; SALDIVA, P. H. N.; SILVA, L. F. F.; NASCIMENTO, L. F. C.; SOLE, D.Background: The prevalence of allergic diseases is increasing. We evaluated temporal trends in the prevalence of asthma, rhinitis and eczema in adolescents (13-14 years) living in Taubate, SP, Brazil (2005-2012) and assessed the relationship between these prevalences and the residential proximity to Presidente Dutra Highway (PDH, a heavily travelled highway). Methods: This cross-sectional study of adolescents (N=1039) from public and private schools was evaluated using the standard questionnaire of the International Study of Asthma and Allergies in Childhood (ISAAC) plus a question about their place of residence in relation to PDH. The data obtained were compared to the 2005 data using a chi-square test or Fisher's exact test. An analysis by groups consisting of two phases (two-step cluster) was used to evaluate the effect of living near PDH. Results: There was a lifetime increase in the prevalence of active asthma (15.3% vs. 20.4%, p = 0.005) and physician-diagnosed asthma (6.8% vs. 9.2%, p = 0.06) and a decrease in the symptoms of active rhinitis (36.6% vs. 18.5%) between 2005 and 2012. A high frequency of asthma and rhinitis (18.1% vs. 23.2%, respectively) was observed among adolescents living close or very close to PDH; furthermore, 85.6% of the adolescents without symptoms of asthma or rhinitis lived far from PDH. Conclusion: An increase in the prevalence of asthma and a decrease in the prevalence of rhinitis were observed during the studied period. Living near PDH was associated with higher rates of asthma, rhinitis, and eczema. (C) 2016 Published by Elsevier Espana, S.L.U. on behalf of SEICAP.
- Nrf2 positively regulates autophagy antioxidant response in human bronchial epithelial cells exposed to diesel exhaust particles(2020) FRIAS, Daniela Perroni; GOMES, Raquel Labiapari Nunes; YOSHIZAKI, Kelly; CARVALHO-OLIVEIRA, Regiani; MATSUDA, Monique; JUNQUEIRA, Mara de Souza; TEODORO, Walcy Rosolia; VASCONCELLOS, Perola de Castro; PEREIRA, Daniela Cristina de Almeida; CONCEICAO, Paulo Roberto da; SALDIVA, Paulo Hilario Nascimento; MAUAD, Thais; MACCHIONE, MariangelaDiesel exhaust particles (DEP) are known to generate reactive oxygen species in the respiratory system, triggering cells to activate antioxidant defence mechanisms, such as Keap1-Nrf2 signalling and autophagy. The aim of this study was to investigate the relationship between the Keap1-Nrf2 signalling and autophagy pathways after DEP exposure. BEAS-2B cells were transfected with silencing RNA (siRNA) specific to Nrf2 and exposed to DEP. The relative levels of mRNA for Nrf2, NQO1, HO-1, LC3B, p62 and Atg5 were determined using RT-PCR, while the levels of LCB3, Nrf2, and p62 protein were determined using Western blotting. The autophagy inhibitor bafilomycin caused a significant decrease in the production of Nrf2, HO-1 and NQO1 compared to DEPs treatment, whereas the Nrf2 activator sulforaphane increased the LC3B (p = 0.020) levels. BEAS-2B cells exposed to DEP at a concentration of 50 mu g/mL for 2 h showed a significant increase in the expression of LC3B (p = 0.001), p62 (p = 0.008), Nrf2 (p = 0.003), HO-1 (p = 0.001) and NQO1 (p = 0.015) genes compared to control. In siRNA-transfected cells, the LC3B (p < 0.001), p62 (p = 0.001) and Atg5 (p = 0.024) mRNA levels and the p62 and LC3II protein levels were decreased, indicating that Nrf2 modulated the expression of autophagy markers (R < 1). These results imply that, in bronchial cells exposed to DEP, the Nrf2 system positively regulates autophagy to maintain cellular homeostasis.
- Chemical composition modulates the adverse effects of particles on the mucociliary epithelium(2015) CARVALHO-OLIVEIRA, Regiani; PIRES-NETO, Ruy Camargo; BUSTILLOS, Jose Oscar Viega; MACCHIONE, Mariangela; DOLHNIKOFF, Marisa; SALDIVA, Paulo H. Nascimento; GARCIA, Maria Lucia BuenoOBJECTIVE: We compared the adverse effects of two types of real ambient particles; i.e., total suspended particles from an electrostatic precipitator of a steel mill and fine air particles from an urban ambient particulate matter of 2.5 mu m, on mucociliary clearance. METHOD: Mucociliary function was quantified by mucociliary transport, ciliary beating frequency and the amount of acid and neutral mucous in epithelial cells through morphometry of frog palate preparations. The palates were immersed in one of the following solutions: total suspended particles (0.1 mg/mL), particulate matter 2.5 mm 0.1 mg/mL (PM0.1) or 3.0 mg/mL (PM3.0) and amphibian Ringer's solution (control). Particle chemical compositions were determined by X-ray fluorescence and gas chromatography/mass spectrometry. RESULTS: Exposure to total suspended particles and PM3.0 decreased mucociliary transport. Ciliary beating frequency was diminished by total suspended particles at all times during exposure, while particulate matter of 2.5 mm did not elicit changes. Particulate matter of 2.5 mm reduced epithelial mucous and epithelium thickness, while total suspended particles behaved similarly to the control group. Total suspended particles exhibited a predominance of Fe and no organic compounds, while the particulate matter 2.5 mm contained predominant amounts of S, Fe, Si and, to a lesser extent, Cu, Ni, V, Zn and organic compounds. CONCLUSION: Our results showed that different compositions of particles induced different airway epithelial responses, emphasizing that knowledge of their individual characteristics may help to establish policies aimed at controlling air pollution.
- Atmospheric metal pollutants and environmental injustice: A methodological approach to environmental risk analysis using fuzzy logic and tree bark(2016) GURGATZ, Bruno Martins; CARVALHO-OLIVEIRA, Regiani; OLIVEIRA, Daniel Canavese de; JOUCOSKI, Emerson; ANTONIACONI, Gisele; SALDIVA, Paulo Hilario do Nascimento; REIS, Rodrigo ArantesAir pollution is the single largest environmental risk today and is increasing in developing countries. In addition, exposure to air pollution is correlated to poor socioeconomic conditions owing to political processes and cultural and historic occupation of land. Ports have several structures that are potential emitters of pollutants such as large ship engines, diesel trucks, and trains. Owing to the considerable costs of implementing direct monitoring networks, alternatives like biomonitoring are an interesting approach to evaluate the environmental status of a particular area using living organisms or their parts such as bark, even though the use of bark as a biomonitor has several problems such as difficulties in determining the exposure period and its correlation with human exposure. Therefore, the use of a complementary mathematical logic is necessary. This study describes a methodological approach to evaluate the environmental risk from air pollution integrating data on environmental pollutants from tree bark using Fuzzy logic, based in the port city of Paranagua in the state of Parana in Brazil, and validated using income indicators. The results indicate that the risk distribution patterns have an inverse relationship with the income indicator, i.e., higher risk levels indicate lower income levels and vice versa. It was concluded that the system was able to identify the distribution of risk and that there is a context of environmental injustice in the region, where the environmental risk related to air pollution is inversely proportional to income levels. This type of information provides a decision making tool for environmental risk analysis from air pollution and can be used in the definition of public policies.
- In vitro mucus transportability, cytogenotoxicity, and hematological changes as non-destructive physiological biomarkers in fish chronically exposed to metals(2015) SERIANI, Robson; ABESSA, Denis M. S.; MOREIRA, Lucas B.; CABRERA, Joana P. G.; SANCHES, Juliana Q.; SILVA, Carolina L. S.; AMORIM, Francisca A.; RIVERO, Dolores H. R. F.; SILVA, Flavia L.; FITORRA, Lilian S.; CARVALHO-OLIVEIRA, Regiani; MACCHIONE, Mariangela; RANZANI-PAIVA, Maria J. T.The biomonitoring of fish using biomarkers represents a useful tool for the assessment of aquatic pollution. This study evaluated the sublethal toxic effects of aquatic pollution on fish collected from a site contaminated by metals. Water and fish (Oreochromis niloticus) samples were collected from a pond in the Parque Ecologico do Tiete (PET) that lies along the Tiete River (Sao Paulo, Brazil), and from a control site (an experimental fish farm). The metal content of the water was evaluated, and fish were used to examine the properties of gill mucus and blood. The PET fish were evaluated for alterations in the in vitro transportability of mucus and changes in blood properties (e.g., cell volume, hemoglobin concentration, red blood cells, and white blood cell count). The results of the water analyzes indicated metal levels above the legal standards for Fe (0.71 mg/L), Ni (0.06 mg/L), Mn (0.11 mg/L), and Pb (0.48 mg/L). Compared to the controls, the hematologic parameter analyzes of PET fish revealed significantly higher numbers of erythrocytes (RBC), leukocytes (WBC), lymphocytes, erythroblasts, and Mean Corpuscular Volume (MCV); however, the hemoglobin content and Mean Corpuscular Hemoglobin Concentration (MCHC) values were significantly lower. The frequencies of nuclear abnormalities and micronuclei were significantly higher and the mucociliary transport was significantly lower in PET fish than in the controls. These results suggest that fish from the contaminated site exhibit a series of physiological responses, which probably indicate health disturbances. Furthermore, the results suggest that blood and mucus are promising, non-destructive targets for use in the monitoring of pollution.