EDER CARLOS ROCHA QUINTAO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina - Docente
LIM/10 - Laboratório de Lípides, Hospital das Clínicas, Faculdade de Medicina
LIM/10 - Laboratório de Lípides, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
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conferenceObject EFFECTS OF LIPC VARIANTS (RS1800558 AND RS2070895) ON HDL-C, HDL SIZE AND HEPATIC LIPASE ACTIVITY IN A REPRESENTATIVE BRAZILIAN POPULATION(2014) SCHERRER, D. Z.; ZAGO, V. H. S.; VIEIRA, I. C.; PARRA, E. S.; PANZOLDO, N. B.; VIRGINIO, V. W. M.; ALEXANDRE, F.; QUINTAO, E. C. R.; FARIA, E. C. de- HDL Size is More Accurate than HDL Cholesterol to Predict Carotid Subclinical Atherosclerosis in Individuals Classified as Low Cardiovascular Risk(2014) PARRA, Eliane Soler; PANZOLDO, Natalia Baratella; ZAGO, Vanessa Helena de Souza; SCHERRER, Daniel Zanetti; ALEXANDRE, Fernanda; BAKKARAT, Jamal; NUNES, Valeria Sutti; NAKANDAKARE, Edna Regina; QUINTAO, Eder Carlos Rocha; NADRUZ- JR., Wilson; FARIA, Eliana Cotta de; SPOSITO, Andrei C.Background: Misclassification of patients as low cardiovascular risk (LCR) remains a major concern and challenges the efficacy of traditional risk markers. Due to its strong association with cholesterol acceptor capacity, high-density lipoprotein (HDL) size has been appointed as a potential risk marker. Hence, we investigate whether HDL size improves the predictive value of HDL-cholesterol in the identification of carotid atherosclerotic burden in individuals stratified to be at LCR. Methods and Findings: 284 individuals (40-75 years) classified as LCR by the current US guidelines were selected in a three-step procedure from primary care centers of the cities of Campinas and Americana, SP, Brazil. Apolipoprotein B-containing lipoproteins were precipitated by polyethylene glycol and HDL size was measured by dynamic light scattering (DLS) technique. Participants were classified in tertiles of HDL size (<7.57; 7.57-8.22; >8.22 nm). Carotid intima-media thickness (cIMT) <0.90 mm (80th percentile) was determined by high resolution ultrasonography and multivariate ordinal regression models were used to assess the association between cIMT across HDL size and levels of lipid parameters. HDLcholesterol was not associated with cIMT. In contrast, HDL size >8.22 nm was independently associated with low cIMT in either unadjusted and adjusted models for age, gender and Homeostasis Model Assessment 2 index for insulin sensitivity, ethnicity and body mass index (Odds ratio 0.23; 95% confidence interval 0.07-0.74, p=0.013). Conclusion: The mean HDL size estimated with DLS constitutes a better predictor for subclinical carotid atherosclerosis than the conventional measurements of plasma HDL-cholesterol in individuals classified as LCR.
conferenceObject ABCA1, ABCG1 AND SCARB1 GENE EXPRESSION ANALYSES IN ASYMPTOMATIC SUBJECTS WITH HIGH AND LOW HDL-C(2014) ZAGO, V. H. S.; SCHERRER, D. Z.; PARRA, E. S.; PANZOLDO, N. B.; VENDRAME, F.; QUINTAO, E. C. R.; FARIA, E. C. de- Increased 27-hydroxycholesterol plasma level in men with low high density lipoprotein-cholesterol may circumvent their reduced cell cholesterol efflux rate(2014) NUNES, Valeria S.; PANZOLDO, Natalia B.; LEANCA, Camila C.; PARRA, Eliane S.; ZAGO, Vanessa S.; SILVA, Eliton J. da; CAZITA, Patricia M.; NAKANDAKARE, Edna R.; FARIA, Eliana C. de; QUINTAO, Eder C. R.Background: HDL is considered the most important mechanism for the excretion of intracellular cholesterol. The liver is the only organ capable to metabolize cholesterol into bile acid. The enzymatic conversion of cholesterol to bile acid is dependent on the cytochrome P450 microsomal system which is also responsible for the generation of oxysterols. The latter's plasma concentrations may reflect the metabolic processes of specific tissues where they are generated. The objective of this study was to investigate in healthy individuals who differ according to their HDL levels the concentration of oxysterols and relate it to the HDL-dependent cell cholesterol efflux rate. Methods: 24-Hydroxycholesterol, 25-hydroxycholesterol, 27-hydroxycholesterol were determined in plasma by GLC/mass spectrometry in 107 healthy subjects with low HDL (HDL-C < 1.03 mmol/l) and high HDL cholesterol (HDL-C > 1.55 mmol/l). HDL-dependent in vitro cell cholesterol efflux rate was measured in 29 cases. Results: No differences were found in plasma oxysterol concentrations between the Low HDL and High HDL groups. There was a significant negative correlation between HDL-C and 27-hydroxycholesterol. Plasma oxysterol concentrations were significantly lower in female than in male subjects. The Low HDL male group had higher 27-hydroxycholesterol than the High HDL male group. Cell cholesterol efflux rate was lower in Low HDL than in High HDL and related inversely with 27-hydroxycholesterol. Conclusion: As compared to High HDL, Low HDL men have increased 27-hydroxycholesterol plasma level that may circumvent their reduced cell cholesterol efflux rate.
- Chemical modification of high density lipoprotein subfractions - HDL2 and HDL3 - after use of atorvastatin(2014) ZAGO, Vanessa Helena de Souza; TANUS-SANTOS, Jose Eduardo; DANELON, Mirian Regina Gardin; PARRA, Eliane Soler; ALEXANDRE, Fernanda; VIEIRA, Isabela Calanca; PANZOLDO, Natalia Baratella; D'ALEXANDRI, Fabio Luis; QUINTAO, Eder Carlos Rocha; FARIA, Eliana Cotta deRegardless of its effect on the concentrations of serum cholesterol, statins exert pleiotropic effects, including the regulation of endothelial function, reduced oxidative stress and inflammation, as well as a slight improvement in the concentrations of high density lipoprotein (HDL). However, its role on the composition of HDL is not yet established. The aim of this study was to evaluate the composition of HDL subfractions, HDL2 and HDL3, after 14 days of placebo and atorvastatin (10 mg/day) use in 30 asymptomatic volunteers. The serum parameters and the HDL subfractions compositions were determined using radiometric, nephelometric and biochemical enzymatic methods. We observed significant reductions of total cholesterol, low density lipoprotein (LDL) and apolipoprotein B-100 by 28%, 40% and 38%, respectively. The analyses of chemical composition of the subfractions revealed a lower lipid protein ratio in HDL2, suggesting enrichment in proteins, and also lower in HDL3, probably by an increase in the number of particles. Several mechanisms can be suggested for the effects observed after the use of atorvastatin, such as a possible action on the reverse cholesterol transport (decreased activity of hepatic lipase and increased phospholipid transfer protein, PLTP), which would explain the enrichment of HDL. The results suggest that statin use may be relevant in the primary prevention of atherosclerosis not only by its lowering effect on LDL-cholesterol and its anti-inflammatory effect but also by beneficial changes in HDL subfractions.
conferenceObject EVIDENCE OF A PROTECTIVE LIPID PROFILE IN THE PRESENCE OF C.+83C > T SNP IN APOLIPOPROTEIN A-I GENE IN A HEALTHY BRAZILIAN POPULATION(2014) PARRA, E. Soler; ZAGO, V. H. Souza; SCHERRER, D. Zanetti; ALEXANDRE, F.; PANZOLDO, N. Baratella; NUNES, V. Sutti; NAKANDAKARE, E. R.; QUINTAO, E. C. R.; FARIA, E. Cotta de- After all plant sterol feeding protects against atherosclerosis(2014) LOTTENBERG, A. M.; BOMBO, R. P.; NAKANDAKARE, E.; QUINTAO, E. R.