Assessing the impact of engineered nanoparticles on wound healing using a novel in vitro bioassay

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art_ZHOU_Assessing_the_impact_of_engineered_nanoparticles_on_wound_2014.PDF (4.69 MB)
Citações na Scopus
26
Tipo de produção
article
Data de publicação
2014
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
FUTURE MEDICINE LTD
Autores
ZHOU, Enhua H.
WATSON, Christa
PIZZO, Richard
COHEN, Joel
Quynh Dang
PARK, Chan Young
CHEN, Cheng
BRAIN, Joseph D.
BUTLER, James P.
Citação
NANOMEDICINE, v.9, n.18, p.2803-2815, 2014
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Aim: As engineered nanoparticles (ENPs) increasingly enter consumer products, humans become increasingly exposed. The first line of defense against ENPs is the epithelium, the integrity of which can be compromised by wounds induced by trauma, infection, or surgery, but the implications of ENPs on wound healing are poorly understood. Materials & methods: Herein, we developed an in vitro assay to assess the impact of ENPs on the wound healing of cells from human cornea. Results & discussion: We show that industrially relevant ENPs impeded wound healing and cellular migration in a manner dependent on the composition, dose and size of the ENPs as well as cell type. CuO and ZnO ENPs impeded both viability and wound healing for both fibroblasts and epithelial cells. Carboxylated polystyrene ENPs retarded wound healing of corneal fibroblasts without affecting viability. Conclusion: Our results highlight the impact of ENPs on cellular wound healing and provide useful tools for studying the physiological impact of ENPs.
Palavras-chave
corneal wound healing, engineered nanoparticles, high-throughput screening, nano-EHS, nanotechnology, nanotoxicology
URI
https://observatorio.fm.usp.br/handle/OPI/9117
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Coleções
Artigos e Materiais de Revistas Científicas - FM/Outros