Proteomic profiling of the proteolytic events in the secretome of the transformed phenotype of melanocyte-derived cells using Terminal Amine Isotopic Labeling of Substrates

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorLIBERATO, Tarcisio
dc.contributor.authorFUKUSHIMA, Isabella
dc.contributor.authorKITANO, Eduardo S.
dc.contributor.authorSERRANO, Solange M. T.
dc.contributor.authorCHAMMAS, Roger
dc.contributor.authorZELANIS, Andre
dc.date.accessioned2019-02-21T17:23:33Z
dc.date.available2019-02-21T17:23:33Z
dc.date.issued2019
dc.description.abstractThe comprehensive profiling of the repertoire of secreted proteins from cancer cells samples provides information on the signaling events in oncogenesis as well as on the cross-talk between normal and tumoral cells. Moreover, the analysis of post-translational modifications in secreted proteins may unravel biological circuits regulated by irreversible modifications such as proteolytic processing. In this context, we used Terminal Amine Isotopic Labeling of Substrates (TAILS) to perform a system-wide investigation on the N-terminome of the secretomes derived from a paired set of mouse cell lines: Melan-a (a normal melanocyte) and Tm1 (its transformed phenotype). Evaluation of the amino acid identities at the scissile bond in internal peptides revealed significant differences, suggesting distinct proteolytic processes acting in the normal and tumoral secretomes. The mapping and annotation of cleavage sites in the tumoral secretome suggested functional roles of active proteases in central biological processes related to oncogenesis, such as the processing of growth factors, cleavage of extracellular matrix proteins and the shedding of ectopic domains from the cell surface, some of which may represent novel processed forms of the corresponding proteins. In the context of the tumor microenvironment, these results suggest important biological roles of proteolytic processing in murine melanoma secreted proteins.eng
dc.description.indexMEDLINEeng
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2013/07467-1, 2014/06579-3, 2015/18096-0, 2017/07897-7]
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.identifier.citationJOURNAL OF PROTEOMICS, v.192, p.291-298, 2019
dc.identifier.doi10.1016/j.jprot.2018.09.010
dc.identifier.eissn1876-7737
dc.identifier.issn1874-3919
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/30827
dc.language.isoeng
dc.publisherELSEVIER SCIENCE BVeng
dc.relation.ispartofJournal of Proteomics
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright ELSEVIER SCIENCE BVeng
dc.subjectSecretomeeng
dc.subjectTAILSeng
dc.subjectN-terminomicseng
dc.subjectProteomicseng
dc.subjectMelanomaeng
dc.subjectCancereng
dc.subject.otherprotein n-terminieng
dc.subject.othertgf-betaeng
dc.subject.otheractive-siteeng
dc.subject.othercancereng
dc.subject.otherdatabaseeng
dc.subject.othertailseng
dc.subject.otheridentificationeng
dc.subject.otherassociationeng
dc.subject.otherenrichmenteng
dc.subject.otherresistanceeng
dc.subject.wosBiochemical Research Methodseng
dc.titleProteomic profiling of the proteolytic events in the secretome of the transformed phenotype of melanocyte-derived cells using Terminal Amine Isotopic Labeling of Substrateseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.author.externalLIBERATO, Tarcisio:Fed Univ Sao Paulo ICT UNIFESP, Dept Sci & Technol, Funct Prote Lab, Sao Jose Dos Campos, SP, Brazil
hcfmusp.author.externalFUKUSHIMA, Isabella:Fed Univ Sao Paulo ICT UNIFESP, Dept Sci & Technol, Funct Prote Lab, Sao Jose Dos Campos, SP, Brazil
hcfmusp.author.externalKITANO, Eduardo S.:Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Lab Especial Toxinol Aplicada, Sao Paulo, Brazil; Inst Butantan, Ctr Excellence New Target Discovery CENTD, Sao Paulo, Brazil
hcfmusp.author.externalSERRANO, Solange M. T.:Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Lab Especial Toxinol Aplicada, Sao Paulo, Brazil
hcfmusp.author.externalZELANIS, Andre:Fed Univ Sao Paulo ICT UNIFESP, Dept Sci & Technol, Funct Prote Lab, Sao Jose Dos Campos, SP, Brazil
hcfmusp.citation.scopus4
hcfmusp.contributor.author-fmusphcROGER CHAMMAS
hcfmusp.description.beginpage291
hcfmusp.description.endpage298
hcfmusp.description.volume192
hcfmusp.origemWOS
hcfmusp.origem.pubmed30267877
hcfmusp.origem.scopus2-s2.0-85054189877
hcfmusp.origem.wosWOS:000455690700026
hcfmusp.publisher.cityAMSTERDAMeng
hcfmusp.publisher.countryNETHERLANDSeng
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