Musashi-1 regulates cell cycle and confers resistance to cisplatin treatment in Group 3/4 medulloblastomas cells

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorCHAGAS, Pablo Shimaoka
dc.contributor.authorVERONEZ, Luciana Chain
dc.contributor.authorSOUSA, Graziella Ribeiro de
dc.contributor.authorCRUZEIRO, Gustavo Alencastro Veiga
dc.contributor.authorCORREA, Carolina Alves Pereira
dc.contributor.authorSAGGIORO, Fabiano Pinto
dc.contributor.authorQUEIROZ, Rosane Gomes de Paula
dc.contributor.authorMARIE, Suely Kazue Nagahashi
dc.contributor.authorBRANDALISE, Silvia Regina
dc.contributor.authorCARDINALLI, Izilda Aparecida
dc.contributor.authorYUNES, Jose Andres
dc.contributor.authorCARLOTTI, Carlos Gilberto
dc.contributor.authorMACHADO, Helio Rubens
dc.contributor.authorSANTOS, Marcelo Volpon
dc.contributor.authorSCRIDELI, Carlos Alberto
dc.contributor.authorTONE, Luiz Gonzaga
dc.contributor.authorVALERA, Elvis Terci
dc.date.accessioned2023-12-15T18:54:06Z
dc.date.available2023-12-15T18:54:06Z
dc.date.issued2023
dc.description.abstractGroups (Grp) 3 and 4 are aggressive molecular subgroups of medulloblastoma (MB), with high rates of leptomeningeal dissemination. To date, there is still a paucity of biomarkers for these subtypes of MBs. In this study, we investigated the clinical significance and biological functions of Musashi-1 (MSI1) in Grp3 and Grp4-MBs. First, we assessed the expression profile of MSI1 in 59 primary MB samples (15-WNT, 18-SHH, 9-Grp3, and 17-Grp4 subgroups) by qRT-PCR. MSI1 mRNA expression levels were also validated in an additional public dataset of MBs (GSE85217). The ROC curve was used to validate the diagnostic standards of MSI1 expression. Next, the potential correlated cell-cycle genes were measured by RNA-Seq. Cell cycle, cell viability, and apoptosis were evaluated in a Grp3/Grp4 MB cell line after knockdown of MSI1 and cisplatin treatment. We identified an overexpression of MSI1 with a high accuracy to discriminate Grp3/Grp4-MBs from non-Grp3/Grp4-MBs. We identified that MSI1 knockdown not only triggered transcriptional changes in the cell-cycle pathway, but also affected G2/M phase in vitro, supporting the role of knockdown of MSI1 in cell-cycle arrest. Finally, MSI1 knockdown decreased cell viability and sensitized D283-Med cells to cisplatin treatment by enhancing cell apoptosis. Based on these findings, we suggest that MSI1 modulates cell-cycle progression and may play a role as biomarker for Grp3/Grp4-MBs. In addition, MSI1 knockdown combined with cisplatin may offer a potential strategy to be further explored in Grp3/Grp4-MBs.eng
dc.description.indexMEDLINE
dc.description.indexPubMed
dc.description.indexWoS
dc.description.indexScopus
dc.identifier.citationHUMAN CELL, v.36, n.6, p.2129-2139, 2023
dc.identifier.doi10.1007/s13577-023-00954-y
dc.identifier.eissn1749-0774
dc.identifier.issn0914-7470
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/57460
dc.language.isoeng
dc.publisherSPRINGER JAPAN KKeng
dc.relation.ispartofHuman Cell
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright SPRINGER JAPAN KKeng
dc.subjectMedulloblastomaeng
dc.subjectGrp3-MBeng
dc.subjectGrp4-MBseng
dc.subjectRNA-binding proteineng
dc.subjectMusashi-1eng
dc.subject.otherbinding protein musashi1eng
dc.subject.otherrnaeng
dc.subject.otherexpressioneng
dc.subject.otherestablishmenteng
dc.subject.othercomplexeng
dc.subject.otherlineeng
dc.subject.wosCell Biologyeng
dc.titleMusashi-1 regulates cell cycle and confers resistance to cisplatin treatment in Group 3/4 medulloblastomas cellseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.affiliation.countryEstados Unidos
hcfmusp.affiliation.countryisous
hcfmusp.author.externalCHAGAS, Pablo Shimaoka:Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Bandeirantes Ave 3900, BR-14048900 Ribeirao Preto, SP, Brazil
hcfmusp.author.externalVERONEZ, Luciana Chain:Univ Sao Paulo, Clin Hosp, Ribeirao Preto Med Sch, Dept Pediat, Ribeirao Preto, Brazil
hcfmusp.author.externalSOUSA, Graziella Ribeiro de:Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Bandeirantes Ave 3900, BR-14048900 Ribeirao Preto, SP, Brazil
hcfmusp.author.externalCRUZEIRO, Gustavo Alencastro Veiga:Univ Sao Paulo, Clin Hosp, Ribeirao Preto Med Sch, Dept Pediat, Ribeirao Preto, Brazil; Harvard Med Sch, Dana Farber Canc Inst, Dept Pediat Oncol, Boston, MA USA; Broad Inst MIT & Harvard, Cambridge, MA USA
hcfmusp.author.externalCORREA, Carolina Alves Pereira:Univ Sao Paulo, Clin Hosp, Ribeirao Preto Med Sch, Dept Pediat, Ribeirao Preto, Brazil
hcfmusp.author.externalSAGGIORO, Fabiano Pinto:Ribeirao Preto Med Sch, Dept Pathol, 3900 Bandeirantes Ave, BR-14049900 Ribeirao Preto, SP, Brazil; Rede Dor Sao Luiz Hosp, Dept Pathol, Rua Perobas, BR-04321120 Sao Paulo, SP, Brazil
hcfmusp.author.externalQUEIROZ, Rosane Gomes de Paula:Univ Sao Paulo, Clin Hosp, Ribeirao Preto Med Sch, Dept Pediat, Ribeirao Preto, Brazil
hcfmusp.author.externalBRANDALISE, Silvia Regina:Boldrinis Children Ctr, Campinas, SP, Brazil
hcfmusp.author.externalCARDINALLI, Izilda Aparecida:Boldrinis Children Ctr, Campinas, SP, Brazil
hcfmusp.author.externalYUNES, Jose Andres:Boldrinis Children Ctr, Campinas, SP, Brazil
hcfmusp.author.externalCARLOTTI, Carlos Gilberto:Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Surg & Anat, Sao Paulo, Brazil
hcfmusp.author.externalMACHADO, Helio Rubens:Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Surg & Anat, Div Pediat Neurosurg, Ribeirao Preto, SP, Brazil
hcfmusp.author.externalSANTOS, Marcelo Volpon:Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Bandeirantes Ave 3900, BR-14049900 Ribeirao Preto, SP, Brazil
hcfmusp.author.externalSCRIDELI, Carlos Alberto:Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Bandeirantes Ave 3900, BR-14048900 Ribeirao Preto, SP, Brazil; Univ Sao Paulo, Clin Hosp, Ribeirao Preto Med Sch, Dept Pediat, Ribeirao Preto, Brazil
hcfmusp.author.externalTONE, Luiz Gonzaga:Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Bandeirantes Ave 3900, BR-14048900 Ribeirao Preto, SP, Brazil; Univ Sao Paulo, Clin Hosp, Ribeirao Preto Med Sch, Dept Pediat, Ribeirao Preto, Brazil
hcfmusp.author.externalVALERA, Elvis Terci:Univ Sao Paulo, Clin Hosp, Ribeirao Preto Med Sch, Dept Pediat, Ribeirao Preto, Brazil
hcfmusp.citation.scopus0
hcfmusp.contributor.author-fmusphcSUELY KAZUE NAGAHASHI MARIE
hcfmusp.description.beginpage2129
hcfmusp.description.endpage2139
hcfmusp.description.issue6
hcfmusp.description.volume36
hcfmusp.origemWOS
hcfmusp.origem.pubmed37460706
hcfmusp.origem.scopus2-s2.0-85164972537
hcfmusp.origem.wosWOS:001028835000001
hcfmusp.publisher.cityTOKYOeng
hcfmusp.publisher.countryJAPANeng
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