Fat Grafts Supplemented with Adipose-Derived Stromal Cells in the Rehabilitation of Patients with Craniofacial Microsomia

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorTANIKAWA, Daniela Y. S.
dc.contributor.authorAGUENA, Meire
dc.contributor.authorBUENO, Daniela F.
dc.contributor.authorPASSOS-BUENO, Maria Rita
dc.contributor.authorALONSO, Nivaldo
dc.date.accessioned2014-04-25T21:49:50Z
dc.date.available2014-04-25T21:49:50Z
dc.date.issued2013
dc.description.abstractBackground: Although first reports of the clinical use of adipose-derived stromal cells suggest that this approach may be feasible and effective for soft-tissue augmentation, there is a lack of randomized, controlled clinical trials in the literature. Thus, this study aimed to investigate whether a faster protocol for isolation of adipose-derived stromal cells and their use in combination with fat tissue improve the long-term retention of the grafts in patients with craniofacial microsomia. Methods: Patients with craniofacial microsomia (n = 14) were grafted either with supplementation of adipose-derived stromal cells (experimental group) or without supplementation of adipose-derived stromal cells (control group). The number of viable cells isolated before and after the supplementation of the grafts was calculated, and these cells were examined for mesenchymal cell surface markers using flow cytometry. Computed tomography was performed to assess both hemifaces preoperatively and at 6 months postoperatively. Results: The average number of viable cells isolated before and after the supplementation of the grafts was 5.6 x 10(5) and 9.9 x 10(5) cells/ml of fat tissue (p = 0.015). Flow cytometric analysis revealed that the adipose-derived stromal cells were positive for mesenchymal cell markers (>95 percent for CD73 and CD105). Surviving fat volume at 6 months was 88 percent for the experimental group and 54 percent for the control group (p = 0.003). Conclusion: These results suggest that this strategy for isolation and supplementation of adipose-derived stromal cells is effective, safe, and superior to conventional lipoinjection for facial recontouring in patients with craniofacial microsomia.
dc.description.indexMEDLINE
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipNational Council for Scientific and Technological Development
dc.identifier.citationPLASTIC AND RECONSTRUCTIVE SURGERY, v.132, n.1, p.141-152, 2013
dc.identifier.doi10.1097/PRS.0b013e3182910a82
dc.identifier.eissn1529-4242
dc.identifier.issn0032-1052
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/5046
dc.language.isoeng
dc.publisherLIPPINCOTT WILLIAMS & WILKINS
dc.relation.ispartofPlastic and Reconstructive Surgery
dc.rightsrestrictedAccess
dc.rights.holderCopyright LIPPINCOTT WILLIAMS & WILKINS
dc.subject.othersoft-tissue augmentation
dc.subject.othermesenchymal stem-cells
dc.subject.otherassisted lipotransfer
dc.subject.otherhemifacial microsomia
dc.subject.otherfacial contour
dc.subject.otherreconstructive surgery
dc.subject.othercellular-components
dc.subject.othersupportive use
dc.subject.othergrowth-factor
dc.subject.othertransplantation
dc.subject.wosSurgery
dc.titleFat Grafts Supplemented with Adipose-Derived Stromal Cells in the Rehabilitation of Patients with Craniofacial Microsomia
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.citation.scopus107
hcfmusp.contributor.author-fmusphcDANIELA YUKIE SAKAI TANIKAWA
hcfmusp.contributor.author-fmusphcNIVALDO ALONSO
hcfmusp.description.beginpage141
hcfmusp.description.endpage152
hcfmusp.description.issue1
hcfmusp.description.volume132
hcfmusp.origemWOS
hcfmusp.origem.pubmed23806916
hcfmusp.origem.scopus2-s2.0-84879964313
hcfmusp.origem.wosWOS:000331726400049
hcfmusp.publisher.cityPHILADELPHIA
hcfmusp.publisher.countryUSA
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hcfmusp.remissive.sponsorshipFAPESP
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relation.isAuthorOfPublication.latestForDiscovery9910369e-8356-48e6-8d2d-267ffee52598
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