A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorTHEODORO-JUNIOR, Osmar Aparecido
dc.contributor.authorRIGHETTI, Renato Fraga
dc.contributor.authorALMEIDA-REIS, Rafael
dc.contributor.authorMARTINS-OLIVEIRA, Bruno Tadeu
dc.contributor.authorOLIVA, Leandro Vilela
dc.contributor.authorPRADO, Carla Maximo
dc.contributor.authorSARAIVA-ROMANHOLO, Beatriz Mangueira
dc.contributor.authorLEICK, Edna Aparecida
dc.contributor.authorPINHEIRO, Nathalia Montouro
dc.contributor.authorLOBO, Yara Aparecida
dc.contributor.authorMARTINS, Milton de Arruda
dc.contributor.authorOLIVA, Maria Luiza Vilela
dc.contributor.authorTIBERIO, Iolanda de Fatima Lopes Calvo
dc.date.accessioned2017-06-09T15:18:22Z
dc.date.available2017-06-09T15:18:22Z
dc.date.issued2017
dc.description.abstractProteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for emphysema. Our aim was to evaluate the effects of a plant Kunitz proteinase inhibitor, Enterolobium contortisiliquum trypsin inhibitor (EcTI), on several aspects of experimental elastase-induced pulmonary inflammation in mice. C57/Bl6 mice were intratracheally administered elastase (ELA) or saline (SAL) and were treated intraperitoneally with EcTI (ELA-EcTI, SAL-EcTI) on days 1, 14 and 21. On day 28, pulmonary mechanics, exhaled nitric oxide (ENO) and number leucocytes in the bronchoalveolar lavage fluid (BALF) were evaluated. Subsequently, lung immunohistochemical staining was submitted to morphometry. EcTI treatment reduced responses of the mechanical respiratory system, number of cells in the BALF, and reduced tumor necrosis factor- (TNF-), matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase-12 (MMP-12), tissue inhibitor of matrix metalloproteinase (TIMP-1), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS)-positive cells and volume proportion of isoprostane, collagen and elastic fibers in the airways and alveolar walls compared with the ELA group. EcTI treatment reduced elastase induced pulmonary inflammation, remodeling, oxidative stress and mechanical alterations, suggesting that this inhibitor may be a potential therapeutic tool for chronic obstructive pulmonary disease (COPD) management.
dc.description.indexMEDLINE
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.identifier.citationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.18, n.2, article ID 403, 18p, 2017
dc.identifier.doi10.3390/ijms18020403
dc.identifier.issn1422-0067
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/19952
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.rightsopenAccess
dc.rights.holderCopyright MDPI AG
dc.subjectCOPD
dc.subjectemphysema
dc.subjectproteinase inhibitor
dc.subjectEcTI
dc.subject.othersmoke-induced emphysema
dc.subject.otherguinea-pigs
dc.subject.otherallergic inflammation
dc.subject.otheranimal-models
dc.subject.otherlung
dc.subject.otherdisease
dc.subject.othercopd
dc.subject.othertissue
dc.subject.otherproteases
dc.subject.otherprotects
dc.subject.wosBiochemistry & Molecular Biology
dc.subject.wosChemistry, Multidisciplinary
dc.titleA Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalTHEODORO-JUNIOR, Osmar Aparecido:Univ Sao Paulo, Sch Med, Dept Clin Med, BR-01246903 Sao Paulo, Brazil
hcfmusp.author.externalALMEIDA-REIS, Rafael:Univ Sao Paulo, Sch Med, Dept Clin Med, BR-01246903 Sao Paulo, Brazil
hcfmusp.author.externalOLIVA, Leandro Vilela:Univ Sao Paulo, Sch Med, Dept Clin Med, BR-01246903 Sao Paulo, Brazil
hcfmusp.author.externalPRADO, Carla Maximo:Univ Fed Sao Paulo, Dept Biosci, BR-09972270 Diadema, Brazil
hcfmusp.author.externalLOBO, Yara Aparecida:Univ Fed Sao Paulo, Dept Biochem, BR-09972270 Diadema, Brazil
hcfmusp.author.externalOLIVA, Maria Luiza Vilela:Univ Fed Sao Paulo, Dept Biochem, BR-09972270 Diadema, Brazil
hcfmusp.citation.scopus23
hcfmusp.contributor.author-fmusphcRENATO FRAGA RIGHETTI
hcfmusp.contributor.author-fmusphcBRUNO TADEU MARTINS DE OLIVEIRA
hcfmusp.contributor.author-fmusphcBEATRIZ MANGUEIRA SARAIVA RAMANHOLO
hcfmusp.contributor.author-fmusphcEDNA APARECIDA LEICK
hcfmusp.contributor.author-fmusphcNATHALIA MONTOURO PINHEIRO MENEGASSO
hcfmusp.contributor.author-fmusphcMILTON DE ARRUDA MARTINS
hcfmusp.contributor.author-fmusphcIOLANDA DE FATIMA LOPES CALVO TIBERIO
hcfmusp.description.articlenumber403
hcfmusp.description.issue2
hcfmusp.description.volume18
hcfmusp.origemWOS
hcfmusp.origem.pubmed28216579
hcfmusp.origem.scopus2-s2.0-85012995010
hcfmusp.origem.wosWOS:000395457700171
hcfmusp.publisher.cityBASEL
hcfmusp.publisher.countrySWITZERLAND
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