Nasopharyngeal carriage of Streptococcus pneumoniae, Haemophilus influenzae, and Staphylococcus aureus in a Brazilian elderly cohort

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art_ZANELLA_Nasopharyngeal_carriage_of_Streptococcus_pneumoniae_Haemophilus_influenzae_and_2019.PDF (559.85 KB)
Citações na Scopus
14
Tipo de produção
article
Data de publicação
2019
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
PUBLIC LIBRARY SCIENCE
Autores
ZANELLA, Rosemeire Cobo
BRANDILEONE, Maria Cristina de Cunto
ALMEIDA, Samanta Cristine Grassi
LEMOS, Ana Paula Silva de
SACCHI, Claudio Tavares
GONCALVES, Claudia R.
GONCALVES, Maria Gisele
FUKASAWA, Lucila Okuyama
Citação
PLOS ONE, v.14, n.8, article ID e0221525, 13p, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
We aimed to investigate the nasopharyngeal colonization (NPC) by Streptococcus pneumoniae, Haemophilus influenzae, and Staphylococcus aureus in the elderly population and to assess the demographic factors associated with NPC. This was an observational cohort study in which outpatients aged >= 60 years were enrolled from April to August 2017, with a follow-up visit from September through December 2017. Nasopharyngeal (NP) swabs were collected, bacteria were detected and isolated, and isolates were subjected to phenotypic and molecular characterization using standard microbiological techniques. At enrolment, the rates of S. aureus, methicillin-resistant S. aureus (MRSA), H. influenzae, and S. pneumoniae among 776 elderly outpatients were 15.9%, 2.3%, 2.5%, and 2.2%, respectively. Toxin production was detected in 21.1% of methicillin-susceptible S. aureus, and three SCCmec types were identified: II/IIb, IVa, and VI. At the follow-up visit, all carriage rates were similar (p > 0.05) to the rates at enrolment. Most of S. pneumoniae serotypes were not included in pneumococcal conjugate vaccines (PCVs), except for 7F, 3, and 19A. All strains of H. influenzae were non-typeable. Previous use of antibiotics and 23-valent pneumococcal polysaccharide vaccination (p < 0.05) were risk factors for S. aureus and MRSA carriage; S. aureus colonization was also associated with chronic kidney disease (p = 0.021). S. pneumoniae carriage was associated with male gender (p = 0.032) and an absence of diabetes (p = 0.034), while not receiving an influenza vaccine (p = 0.049) and chronic obstructive pulmonary disease (p = 0.031) were risk factors for H. influenzae colonization. The frailty of study participants was not associated with colonization status. We found a higher S. aureus carriage rate compared with the S. pneumoniae- and H. influenzae-carriage rates in a well attended population in a geriatric outpatient clinic. This is one of the few studies conducted in Brazil that can support future colonization studies among elderly individuals.
Palavras-chave
URI
https://observatorio.fm.usp.br/handle/OPI/33928
Referências
  1. Abdullahi O, 2008, PEDIATR INFECT DIS J, V27, P59, DOI 10.1097/INF.0b013e31814da70c
  2. Alarcon B, 2006, J APPL MICROBIOL, V100, P352, DOI 10.1111/j.1365-2672.2005.02768.x
  3. Almeida ST, 2015, EUR J CLIN MICROBIOL, V34, P593, DOI 10.1007/s10096-014-2267-8
  4. Andersen PS, 2013, EUR J CLIN MICROBIOL, V32, P1321, DOI 10.1007/s10096-013-1882-0
  5. Becker-Dreps S, 2015, J AM GERIATR SOC, V63, P2094, DOI 10.1111/jgs.13651
  6. Brandileone MCC, 2018, VACCINE, V36, P2559, DOI 10.1016/j.vaccine.2018.04.010
  7. Camarano A, 2010, CUIDADOS LONGA DURAC
  8. Carvalho MDS, 2007, J CLIN MICROBIOL, V45, P2460, DOI 10.1128/JCM.02498-06
  9. Chiefari AK, 2015, MOL CELL PROBE, V29, P461, DOI 10.1016/j.mcp.2015.06.004
  10. Clinical and Laboratory Standards Institute (CLSI), M10027 CLSI
  11. Coughtrie AL, 2014, BMJ OPEN, V4, DOI 10.1136/bmjopen-2014-005341
  12. da Silveira M, 2018, ANN CLIN MICROB ANTI, V17, DOI 10.1186/s12941-018-0271-z
  13. de Brito CS, 2015, REV SOC BRAS MED TRO, V48, P614, DOI 10.1590/0037-8682-0030-2015
  14. Domingues CMAS, 2014, LANCET RESP MED, V2, P464, DOI 10.1016/S2213-2600(14)70060-8
  15. Drayss M, 2019, PLOS ONE, V14, DOI 10.1371/journal.pone.0212052
  16. Dunne EM, 2018, PLOS ONE, V13, DOI 10.1371/journal.pone.0195098
  17. Ensrud KE, 2008, ARCH INTERN MED, V168, P382, DOI 10.1001/archinternmed.2007.113
  18. Esposito S, 2016, IMMUN AGEING, V13, DOI 10.1186/s12979-016-0057-0
  19. Flamaing J, 2010, J AM GERIATR SOC, V58, P396, DOI 10.1111/j.1532-5415.2009.02700.x
  20. Fosheim GE, 2011, J CLIN MICROBIOL, V49, P3071, DOI 10.1128/JCM.00795-11
  21. Fung HB, 2010, AM J GERIATR PHARMAC, V8, P47, DOI 10.1016/j.amjopharm.2010.01.003
  22. Gamblin J, 2013, J MED MICROBIOL, V62, P437, DOI 10.1099/jmm.0.051854-0
  23. Greenberg D, 2004, J CLIN MICROBIOL, V42, P4604, DOI 10.1128/JCM.42.10.4604-4609.2004
  24. Harris PA, 2009, J BIOMED INFORM, V42, P377, DOI 10.1016/j.jbi.2008.08.010
  25. Lewnard JA, 2016, J INFECT DIS, V213, P1596, DOI 10.1093/infdis/jiv761
  26. Liu CL, 2015, J MICROBIOL IMMUNOL, V48, P490, DOI 10.1016/j.jmii.2014.03.003
  27. Maaroufi Y, 2007, J CLIN MICROBIOL, V45, P2305, DOI 10.1128/JCM.00102-07
  28. McClure-Warnier JA, 2013, JOVE-J VIS EXP, DOI 10.3791/50779
  29. Metersky ML, 2012, INT J INFECT DIS, V16, pE321, DOI 10.1016/j.ijid.2012.01.003
  30. Milucky J, 2019, VACCINE, V37, P1094, DOI 10.1016/j.vaccine.2018.12.075
  31. Munckhof WJ, 2009, CLIN MICROBIOL INFEC, V15, P149, DOI 10.1111/j.1469-0691.2008.02652.x
  32. Jimenez JN, 2011, MEM I OSWALDO CRUZ, V106, P980, DOI 10.1590/S0074-02762011000800013
  33. Nzenze SA, 2014, VACCINE, V32, P5520, DOI 10.1016/j.vaccine.2014.06.091
  34. O'Brien Katherine L, 2003, Pediatr Infect Dis J, V22, pe1, DOI 10.1097/00006454-200302000-00010
  35. Palmu AA, 2012, SCAND J INFECT DIS, V44, P433, DOI 10.3109/00365548.2011.652162
  36. Principi N, 2016, HUM VACC IMMUNOTHER, V12, P293, DOI 10.1080/21645515.2015.1072666
  37. Regev-Yochay G, 2004, CLIN INFECT DIS, V38, P632, DOI 10.1086/381547
  38. Scerri J, 2013, J EPIDEMIOL GLOB HEA, V3, P165, DOI 10.1016/j.jegh.2013.05.003
  39. Scribel LV, 2011, REV INST MED TROP SP, V53, P197, DOI 10.1590/S0036-46652011000400004
  40. Van Eldere J, 2014, LANCET INFECT DIS, V14, P1281, DOI 10.1016/S1473-3099(14)70734-0
  41. Wang X, 2012, J CLIN MICROBIOL, V50, P702, DOI 10.1128/JCM.06087-11
  42. Welte T, 2012, THORAX, V67, P71, DOI 10.1136/thx.2009.129502

Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/66
Artigos e Materiais de Revistas Científicas - ODS/03