Rapid antigen detection test for respiratory syncytial virus diagnosis as a diagnostic tool

Página do item simplificado
dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorMESQUITA, Flavio da Silva
dc.contributor.authorOLIVEIRA, Danielle Bruna Leal de
dc.contributor.authorCREMA, Daniela
dc.contributor.authorPINEZ, Celia Miranda Nunes
dc.contributor.authorCOLMANETTI, Thais Cristina
dc.contributor.authorTHOMAZELLI, Luciano Matsumia
dc.contributor.authorGILIO, Alfredo Elias
dc.contributor.authorVIEIRA, Sandra Elisabeth
dc.contributor.authorMARTINEZ, Marina Baquerizo
dc.contributor.authorBOTOSSO, Viviane Fongaro
dc.contributor.authorDURIGON, Edison Luiz
dc.date.accessioned2017-08-17T19:19:33Z
dc.date.available2017-08-17T19:19:33Z
dc.date.issued2017
dc.description.abstractObjective: The aim of this study was to evaluate the QuickVue (R) RSV Test Kit (QUIDEL Corp, CA, USA) as a screening tool for respiratory syncytial virus in children with acute respiratory disease in comparison with the indirect immunofluorescence assay as gold standard. In Brazil, rapid antigen detection tests for respiratory syncytial virus are not routinely utilized as a diagnostic tool, except for the diagnosis of dengue and influenza. Methods: The authors retrospectively analyzed 486 nasopharyngeal aspirate samples from children under age 5 with acute respiratory infection, between December 2013 and August 2014, the samples were analyzed by indirect immunofluorescence assay and QuickVue (R) RSV Test kit. Samples with discordant results were analyzed by real time PCR and nucleotide sequencing. Results: From 313 positive samples by immunofluorescence assays, 282 (90%) were also positive by the rapid antigen detection test, two were positive only by rapid antigen detection test, 33 were positive only by immunofluorescence assays, and 171 were positive by both methods. The 35 samples with discordant results were analyzed by real time PCR; the two samples positive only by rapid antigen detection test and the five positive only by immunofluorescence assays RSV Test and viral load or specific strain. The QuickVue (R) RSV Test showed sensitivity of 90%, specificity of 98.8%, predictive positive value of 99.3%, and negative predictive value of 94.6%, with accuracy of 93.2% and agreement kappa index of 0.85 in comparison to immunofluorescence assay. Conclusions: This study demonstrated that the QuickVue RSV Test Kit can be effective in early detection of Respiratory syncytial virus in nasopharyngeal aspirate and is reliable for use as a diagnostic tool in pediatrics. (C) 2016 Sociedade Brasileira de Pediatria.
dc.description.indexMEDLINE
dc.description.sponsorshipUniversidade de Sao Paulo (USP)
dc.identifier.citationJORNAL DE PEDIATRIA, v.93, n.3, p.246-252, 2017
dc.identifier.doi10.1016/j.jped.2016.06.013
dc.identifier.eissn1678-4782
dc.identifier.issn0021-7557
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/21383
dc.language.isoeng
dc.publisherSOC BRASIL PEDIATRIA
dc.relation.ispartofJornal de Pediatria
dc.rightsopenAccess
dc.rights.holderCopyright SOC BRASIL PEDIATRIA
dc.subjectRespiratory-viruses
dc.subjectRespiratory syncytial virus - RSV
dc.subjectRapid antigen detection test - RADT
dc.subject.othergroup-a
dc.subject.othercirculation patterns
dc.subject.otherinfection
dc.subject.otherbronchiolitis
dc.subject.otherchildren
dc.subject.othercare
dc.subject.othermanagement
dc.subject.otherstrains
dc.subject.otherassays
dc.subject.otherpoint
dc.subject.wosPediatrics
dc.titleRapid antigen detection test for respiratory syncytial virus diagnosis as a diagnostic tool
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalMESQUITA, Flavio da Silva:Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, SP, Brazil
hcfmusp.author.externalOLIVEIRA, Danielle Bruna Leal de:Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, SP, Brazil
hcfmusp.author.externalCREMA, Daniela:Univ Sao Paulo, Hosp Univ, Lab Clin, Sao Paulo, SP, Brazil
hcfmusp.author.externalPINEZ, Celia Miranda Nunes:Univ Sao Paulo, Hosp Univ, Lab Clin, Sao Paulo, SP, Brazil
hcfmusp.author.externalCOLMANETTI, Thais Cristina:Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, SP, Brazil
hcfmusp.author.externalTHOMAZELLI, Luciano Matsumia:Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, SP, Brazil
hcfmusp.author.externalMARTINEZ, Marina Baquerizo:Univ Sao Paulo, Hosp Univ, Lab Clin, Sao Paulo, SP, Brazil; Univ Sao Paulo, Escola Ciencias Farmaceut, Sao Paulo, SP, Brazil
hcfmusp.author.externalBOTOSSO, Viviane Fongaro:Inst Butantan, Lab Virol, Div Desenvolvimento Cient, Sao Paulo, SP, Brazil
hcfmusp.author.externalDURIGON, Edison Luiz:Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, SP, Brazil
hcfmusp.citation.scopus14
hcfmusp.contributor.author-fmusphcALFREDO ELIAS GILIO
hcfmusp.contributor.author-fmusphcSANDRA ELISABETE VIEIRA
hcfmusp.description.beginpage246
hcfmusp.description.endpage252
hcfmusp.description.issue3
hcfmusp.description.volume93
hcfmusp.origemWOS
hcfmusp.origem.pubmed27889321
hcfmusp.origem.scieloSCIELO:S0021-75572017000300246
hcfmusp.origem.scopus2-s2.0-85008222819
hcfmusp.origem.wosWOS:000402692700007
hcfmusp.publisher.cityRIO DE JANEIRO, RJ
hcfmusp.publisher.countryBRAZIL
hcfmusp.relation.referenceALTMAN DG, 1994, BRIT MED J, V309, P996
hcfmusp.relation.referenceChartrand C, 2015, J CLIN MICROBIOL, V53, P3738, DOI 10.1128/JCM.01816-15
hcfmusp.relation.referenceChristakis DA, 2005, PEDIATRICS, V115, P878, DOI 10.1542/peds.2004-1299
hcfmusp.relation.referenceDoan Q, 2012, COCHRANE DB SYST REV, V5
hcfmusp.relation.referenceDomachowske JB, 1999, CLIN MICROBIOL REV, V12, P298
hcfmusp.relation.referenceFerronato AE, 2012, CLINICS, V67, P1001, DOI 10.6061/clinics/2012(09)03
hcfmusp.relation.referenceGLEZEN WP, 1986, AM J DIS CHILD, V140, P543
hcfmusp.relation.referenceHASEGAWA M, 1985, J MOL EVOL, V22, P160, DOI 10.1007/BF02101694
hcfmusp.relation.referenceJunior OCF, 2005, AIDS, V19, pS70
hcfmusp.relation.referenceLANDIS JR, 1977, BIOMETRICS, V33, P159, DOI 10.2307/2529310
hcfmusp.relation.referenceLeonardi GP, 2015, J VIROL METHODS, V213, P131, DOI 10.1016/j.jviromet.2014.11.016
hcfmusp.relation.referenceLieberthal AS, 2006, PEDIATRICS, V118, P1774, DOI 10.1542/peds.2006-2223
hcfmusp.relation.referenceLima HN, 2012, VIRUS RES, V163, P82, DOI 10.1016/j.virusres.2011.08.013
hcfmusp.relation.referenceMacKinnon D P, 2000, Prev Sci, V1, P173, DOI 10.1023/A:1026595011371
hcfmusp.relation.referenceMohapatra SS, 2008, CLIN MICROBIOL REV, V21, P495, DOI 10.1128/CMR.00054-07
hcfmusp.relation.referenceNei M, 2000, MOL EVOLUTION PHYLOG
hcfmusp.relation.referenceOpenshaw PJM, 2002, RESPIR RES, V3, DOI 10.1186/rr184
hcfmusp.relation.referencePecchini R, 2008, BRAZ J INFECT DIS, V12, P476, DOI 10.1590/S1413-86702008000600006
hcfmusp.relation.referencePeret TCT, 1998, J GEN VIROL, V79, P2221
hcfmusp.relation.referencePeret TCT, 2000, J INFECT DIS, V181, P1891, DOI 10.1086/315508
hcfmusp.relation.referenceLima MDQ, 2010, PLOS NEGLECT TROP D, V4, DOI 10.1371/journal.pntd.0000738
hcfmusp.relation.referenceRandolph AG, 2004, PEDIATR INFECT DIS J, V23, P990, DOI 10.1097/01.inf.0000143647.88873.66
hcfmusp.relation.referenceRAY CG, 1987, J CLIN MICROBIOL, V25, P355
hcfmusp.relation.referenceShay DK, 1999, JAMA-J AM MED ASSOC, V282, P1440, DOI 10.1001/jama.282.15.1440
hcfmusp.relation.referenceTamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI 10.1093/molbev/mst197
hcfmusp.relation.referenceTrento A, 2015, J VIROL, V89, P7776, DOI 10.1128/JVI.00467-15
hcfmusp.relation.referenceTuttle R, 2015, DIAGN MICR INFEC DIS, V81, P171, DOI 10.1016/j.diagmicrobio.2014.11.013
hcfmusp.relation.referenceWang EEL, 1999, CLIN INFECT DIS, V29, P155, DOI 10.1086/520145
hcfmusp.relation.referenceZheng HQ, 1996, J CLIN MICROBIOL, V34, P334
hcfmusp.relation.referenceZorc JJ, 2010, PEDIATRICS, V125, P342, DOI 10.1542/peds.2009-2092
hcfmusp.scopus.lastupdate2024-05-10
relation.isAuthorOfPublicationdca43a6e-5d1a-42e3-ae31-b21b4316be2b
relation.isAuthorOfPublication87a0d77a-ee6f-4966-b761-6daf62591643
relation.isAuthorOfPublication.latestForDiscoverydca43a6e-5d1a-42e3-ae31-b21b4316be2b
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
art_MESQUITA_Rapid_antigen_detection_test_for_respiratory_syncytial_virus_2017.PDF
Tamanho:
918.06 KB
Formato:
Adobe Portable Document Format
Descrição:
publishedVersion (English)
Baixar
Coleções
Artigos e Materiais de Revistas Científicas - FM/MPE
Artigos e Materiais de Revistas Científicas - ODS/03