The effects of low-level laser irradiation on bone tissue in diabetic rats

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorPATROCINIO-SILVA, Tatiane Lopes
dc.contributor.authorSOUZA, Andre Moreira Fogaca de
dc.contributor.authorGOULART, Raul Loppi
dc.contributor.authorPEGORARI, Carolina Fuirini
dc.contributor.authorOLIVEIRA, Jussan Rodrigues
dc.contributor.authorFERNANDES, Kelly
dc.contributor.authorMAGRI, Angela
dc.contributor.authorPEREIRA, Rosa Maria Rodrigues
dc.contributor.authorARAKI, Daniel Ribeiro
dc.contributor.authorNAGAOKA, Marcia Regina
dc.contributor.authorPARIZOTTO, Nivaldo Antonio
dc.contributor.authorRENNO, Ana Claudia Muniz
dc.date.accessioned2015-02-06T19:10:28Z
dc.date.available2015-02-06T19:10:28Z
dc.date.issued2014
dc.description.abstractDiabetes mellitus (DM) leads to a decrease in bone mass and increase the risk of osteoporosis and in this context, many treatments have shown to accelerate bone metabolism. It seems that low-level laser therapy (LLLT) is able of stimulating osteoblast activity and produced increased biomechanical properties. However, its effects on bone in diabetic rats are not fully elucidated. The aim of this study was to evaluate the effects of LLLT on bone formation, immunoexpression of osteogenic factors, biomechanical properties and densitometric parameters in diabetic rats. Thirty male Wistar rats were randomly distributed into three experimental groups: control group, diabetic group, and laser-treated diabetic group. DM was induced by streptozotocin (STZ) and after 1 week laser treatment started. An 830-nm laser was used, performed for 18 sessions, during 6 weeks. At the end of the experiment, animals were euthanized and tibias and femurs were defleshed for analysis. Extensive resorptive areas as a result of osteoclasts activity were noticed in DG when compared to control. Laser-treated animals showed an increased cortical area. The immunohistochemical analysis revealed that LLLT produced an increased RUNX-2 expression compared to other groups. Similar RANK-L immunoexpression was observed for all experimental groups. In addition, laser irradiation produced a statistically increase in fracture force, bone mineral content (BMC) and bone mineral density compared to DG. The results of this study indicate that the STZ model was efficient in inducing DM 1 and producing a decrease in cortical diameter, biomechanical properties and in densitometric variables. In addition, it seems that LLLT stimulated bone metabolism, decreased resorptive areas, increased RUNX-2 expression, cortical area, fracture force, BMD, and BMC. Further studies should be developed to provide additional information concerning the mechanisms of action of laser therapy in diabetic bone in experimental and clinical trials.
dc.description.indexMEDLINE
dc.identifier.citationLASERS IN MEDICAL SCIENCE, v.29, n.4, p.1357-1364, 2014
dc.identifier.doi10.1007/s10103-013-1418-y
dc.identifier.eissn1435-604X
dc.identifier.issn0268-8921
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/8327
dc.language.isoeng
dc.publisherSPRINGER LONDON LTD
dc.relation.ispartofLasers in Medical Science
dc.rightsrestrictedAccess
dc.rights.holderCopyright SPRINGER LONDON LTD
dc.subjectDiabetes mellitus
dc.subjectBone tissue
dc.subjectLow-level laser therapy
dc.subject.othermechanical-properties
dc.subject.otherpartial osteotomy
dc.subject.othertherapy
dc.subject.otherdefects
dc.subject.otherinsulin
dc.subject.othertibia
dc.subject.otherphotostimulation
dc.subject.otherapoptosis
dc.subject.otherdiagnosis
dc.subject.othermellitus
dc.subject.wosEngineering, Biomedical
dc.subject.wosSurgery
dc.titleThe effects of low-level laser irradiation on bone tissue in diabetic rats
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalPATROCINIO-SILVA, Tatiane Lopes:Univ Fed Sao Carlos, Dept Biotechnol, BR-13565902 Sao Paulo, Brazil
hcfmusp.author.externalSOUZA, Andre Moreira Fogaca de:Univ Fed Sao Paulo, Dept Physiotherapy, BR-11050240 Sao Paulo, Brazil
hcfmusp.author.externalGOULART, Raul Loppi:Univ Fed Sao Paulo, Dept Physiotherapy, BR-11050240 Sao Paulo, Brazil
hcfmusp.author.externalPEGORARI, Carolina Fuirini:Univ Fed Sao Paulo, Dept Physiotherapy, BR-11050240 Sao Paulo, Brazil
hcfmusp.author.externalOLIVEIRA, Jussan Rodrigues:Univ Fed Sao Paulo, Dept Physiotherapy, BR-11050240 Sao Paulo, Brazil
hcfmusp.author.externalFERNANDES, Kelly:Univ Fed Sao Paulo, Dept Physiotherapy, BR-11050240 Sao Paulo, Brazil
hcfmusp.author.externalMAGRI, Angela:Univ Fed Sao Paulo, Dept Physiotherapy, BR-11050240 Sao Paulo, Brazil
hcfmusp.author.externalARAKI, Daniel Ribeiro:Univ Fed Sao Paulo, Dept Biosci, BR-11050240 Sao Paulo, Brazil
hcfmusp.author.externalNAGAOKA, Marcia Regina:Univ Fed Sao Paulo, Dept Biosci, BR-11050240 Sao Paulo, Brazil
hcfmusp.author.externalPARIZOTTO, Nivaldo Antonio:Univ Fed Sao Carlos, Dept Biotechnol, BR-13565902 Sao Paulo, Brazil
hcfmusp.author.externalRENNO, Ana Claudia Muniz:Univ Fed Sao Paulo, Dept Biosci, BR-11050240 Sao Paulo, Brazil
hcfmusp.citation.scopus23
hcfmusp.contributor.author-fmusphcROSA MARIA RODRIGUES PEREIRA
hcfmusp.description.beginpage1357
hcfmusp.description.endpage1364
hcfmusp.description.issue4
hcfmusp.description.volume29
hcfmusp.origemWOS
hcfmusp.origem.scopus2-s2.0-84903756692
hcfmusp.origem.wosWOS:000338645800006
hcfmusp.publisher.cityLONDON
hcfmusp.publisher.countryENGLAND
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