Efficiency of Casework Direct Kit for extraction of touch DNA samples obtained from cars steering wheels

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorFRIDMAN, Cintia
dc.contributor.authorGONCALVES, Fernanda T.
dc.contributor.authorFRANCISCO, Daniela O.
dc.date.accessioned2020-03-24T14:52:52Z
dc.date.available2020-03-24T14:52:52Z
dc.date.issued2019
dc.description.abstractAnalysis of STR profiles obtained from touch DNA has been very useful to the elucidation of crimes. Extraction method may be determinant for the recovery of genetic material collected from different surfaces. Vehicle theft is one of the most common crimes in Sao Paulo city, Brazil, but collection of biological traces in car steering wheels is not considered, because of the belief that profiles generated won't be able to identify the thief, only the owner. This study aimed to analyze the efficacy of extraction methods for obtaining DNA profiles in samples collected from steering wheels. Eight criminal acts were simulated with 2 different individuals each (mixture of victim and thief), in duplicate, in order to compare two extraction methods: DNA IQ (TM) and Casework Direct Kit (both Promega Corporation). Genetic material was collected by double swab method and quantified by Quantifiler (TM) Trio (ThermoFisher Scientific). Amplification was conducted with PowerPlex (R) Fusion System (Promega). It was possible to obtain STR profiles for all experiments. The mixtures were compared with reference profiles to evaluated how many alleles of each donor were observed. Samples extracted with Casework Direct Kit obtained STR profiles with higher averages of alleles for primary and secondary donors (88.7% and 59.9%, respectively) than those extracted with DNA IQ (TM) (60.4% and 38.1%, respectively). This could be explained by the differences established in the protocols of both methods, since DNA IQ (TM) is based on successive washes and can result in loss of DNA, whereas Casework Direct Kit minimizes this problem. We concluded that Casework Direct Kit was more efficient for processing touch DNA samples than DNA IQ (TM).eng
dc.description.indexPubMedeng
dc.description.sponsorshipFAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [16/14355-3, 17/06484-0]
dc.description.sponsorshipPromega Corporation
dc.description.sponsorshipLIM40/HCFMUSP
dc.identifier.citationFORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES, v.7, n.1, p.16-18, 2019
dc.identifier.doi10.1016/j.fsigss.2019.09.007
dc.identifier.eissn1875-175X
dc.identifier.issn1875-1768
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/35559
dc.language.isoeng
dc.publisherELSEVIER IRELAND LTDeng
dc.relation.ispartofForensic Science International Genetics Supplement Series
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright ELSEVIER IRELAND LTDeng
dc.subjectTouch DNAeng
dc.subjectForensic DNAeng
dc.subjectDNA extractioneng
dc.subject.otherskineng
dc.subject.wosGenetics & Heredityeng
dc.titleEfficiency of Casework Direct Kit for extraction of touch DNA samples obtained from cars steering wheelseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.citation.scopus3
hcfmusp.contributor.author-fmusphcCINTIA FRIDMAN RAVE
hcfmusp.contributor.author-fmusphcFERNANDA DE TOLEDO GONCALVES
hcfmusp.contributor.author-fmusphcDANIELA DE OLIVEIRA FRANCISCO
hcfmusp.description.beginpage16
hcfmusp.description.endpage18
hcfmusp.description.issue1
hcfmusp.description.volume7
hcfmusp.origemWOS
hcfmusp.origem.scopus2-s2.0-85075443868
hcfmusp.origem.wosWOS:000508217000008
hcfmusp.publisher.cityCLAREeng
hcfmusp.publisher.countryIRELANDeng
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hcfmusp.scopus.lastupdate2024-06-09
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