High molecular weight components containing N-linked oligosaccharides of Ascaris suum extract inhibit the dendritic cells activation through DC-SIGN and MR

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art_FAVORETTO_High_molecular_weight_components_containing_Nlinked_oligosaccharides_of_2017.PDF (2.64 MB)
Citações na Scopus
14
Tipo de produção
article
Data de publicação
2017
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
PERGAMON-ELSEVIER SCIENCE LTD
Autores
FAVORETTO, Bruna C.
CASABUONO, Adriana A. C.
PORTES-JUNIOR, Jose A.
COUTO, Alicia S.
FAQUIM-MAURO, Eliana L.
Citação
MOLECULAR IMMUNOLOGY, v.87, p.33-46, 2017
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Helminths, as well as their secretory/excretory products, induce a tolerogenic immune microenvironment. High molecular weight components (PI) from Ascaris suum extract down-modulate the immune response against ovalbumin (OVA). The PI exerts direct effect on dendritic cells (DCs) independent of TLR 2, 4 and MyD88 molecule and, thus, decreases the T lymphocytes response. Here, we studied the glycoconjugates in PI and the role of C-type lectin receptors (CLRs), DC-SIGN and MR, in the modulation of DCs activity. Our data showed the presence of glycoconjugates with high mannose- and complex-type N-linked oligosaccharide chains and phosphorylcholine residues on PI. In addition, these N-linked glycoconjugates inhibited the DCs maturation induced by LPS. The binding and internalization of PI-Alexa were decreased on DCs previously incubated with mannan, anti-DC-SIGN and/or anti-MR antibodies. In agreement with this, the incubation of DCs with mannan, anti-DC-SIGN and/or anti-MR antibodies abolished the down-modulatory effect of PI on these cells. It was also observed that the blockage of CLRs, DC-SIGN and MR on DCs reverted the inhibitory effect of PI in in vitro T cells proliferation. Therefore, our data show the involvement of DC-SIGN and MR in the recognition and consequent modulatory effect of N-glycosylated components of PI on DCs.
Palavras-chave
Ascaris suum, C-type lectin receptors, PI - High molecular weight components, from Ascaris suum extract, DC-Dendritic Cell, DCSIGN-Dendritic cell specifc ICAM-3, grabbing non-integrin, MR-Mannose receptor
URI
https://observatorio.fm.usp.br/handle/OPI/21605
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Coleções
Artigos e Materiais de Revistas Científicas - LIM/62