Performance of immunohistochemistry as a useful tool for the diagnosis of cutaneous leishmaniasis in Panama, Central America

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art_GONZALEZ_Performance_of_immunohistochemistry_as_a_useful_tool_for_2019.PDF (1.22 MB)
Citações na Scopus
9
Tipo de produção
article
Data de publicação
2019
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER IRELAND LTD
Autores
CALZADA, J. E.
DIAZ, R.
PAZ, H.
GARCIA, V.
MIRANDA, A.
PUGA, S.
SALDANA, A.
Citação
PARASITOLOGY INTERNATIONAL, v.71, p.46-52, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Cutaneous leishmaniasis (CL) is one of the most frequent parasitic zoonoses in Panama. Currently, conventional, molecular and histopathological tests are performed to diagnose CL. Immunohistochemistry (IHC) has proven to be a valuable tool to facilitate the diagnosis of leishmaniasis and to study the cellular immune response developed during the infection. Therefore, considering the absence of IHC in the diagnostic routine in Panama, the objective of this study is to demonstrate the usefulness of this test as a complementary diagnostic tool for improving the sensitivity of histopathology (HP) and helping to study the cellular immune response of patients. Samples from patients with suspected CL were analysed by intradermal reaction of Montenegro (IDRM), smears, culture, PCR (Viannia, Hsp-70), HP and IHC. According to the diagnostic criteria, 95.8% of patients were positive for Leishmania sp., that was characterized as Leishmania (V.) panarnensis by PCR-HSP7O/RFLP. From positive samples, Leishmania was detected by the tested diagnostic methods in the following degrees: 100% by IDRM, 60% by smears, 93.3% by culture, 100% by kDNA PCR, 78.3% by PCR Hsp-70, 50% by HP and 73.9% by IHC. Although IHC had a poor correlation (k = 0.191) with the diagnostic criteria, the sensitivities of both HP (76.1%) and smears (89.1%) were improved by combining them with IHC. IHC considerably improved the detection of the Leishmania parasites in the histopathological sections, supporting the need to implement this diagnostic tool in Panama. In addition, immunohistochemistry allows evaluation of the patient's immune response and thus provides new guidelines for the treatment and control of CL in Panama.
Palavras-chave
Cutaneous leishmaniasis, Diagnosis, Immunohistochemistry, L.(V.) panamensis
URI
https://observatorio.fm.usp.br/handle/OPI/32528
Referências
  1. Alves CF, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0063343
  2. Barrio A, 2007, AM J TROP MED HYG, V77, P636, DOI 10.4269/ajtmh.2007.77.636
  3. Benicio ED, 2011, AN BRAS DERMATOL, V86, P1141, DOI 10.1590/S0365-05962011000600012
  4. Bensoussan E, 2006, J CLIN MICROBIOL, V44, P1435, DOI 10.1128/JCM.44.4.1435-1439.2006
  5. Cruz-Barrera ML, 2015, J CLIN MICROBIOL, V53, P1339, DOI 10.1128/JCM.02955-14
  6. de Y., 2010, REV MED CIENTIFICA, V23, P45
  7. Engel KB, 2011, ARCH PATHOL LAB MED, V135, P537, DOI 10.1043/2010-0702-RAIR.1
  8. Goto H, 2010, EXPERT REV ANTI-INFE, V8, P419, DOI [10.1586/eri.10.19, 10.1586/ERI.10.19]
  9. Handler MZ, 2015, J AM ACAD DERMATOL, V73, P897, DOI 10.1016/j.jaad.2014.08.051
  10. Handler MZ, 2015, J AM ACAD DERMATOL, V73, P911, DOI 10.1016/j.jaad.2014.09.014
  11. Kenner JR, 1999, J CUTAN PATHOL, V26, P130, DOI 10.1111/j.1600-0560.1999.tb01817.x
  12. Magalhaes AV, 1986, REV INST MED TROP, V28, P421
  13. Markle WH, 2004, AM FAM PHYSICIAN, V69, P1455
  14. Miranda A, 2012, T ROY SOC TROP MED H, V106, P544, DOI 10.1016/j.trstmh.2012.05.005
  15. Miranda A, 2009, AM J TROP MED HYG, V81, P565, DOI 10.4269/ajtmh.2009.08-0265
  16. Montalvo AM, 2012, EUR J CLIN MICROBIOL, V31, P1453, DOI 10.1007/s10096-011-1463-z
  17. Montalvo AM, 2010, PARASITOLOGY, V137, P1159, DOI 10.1017/S0031182010000089
  18. Alvarez AMM, 2010, T ROY SOC TROP MED H, V104, P364, DOI 10.1016/j.trstmh.2009.12.002
  19. Moreira MAB, 2007, VET PARASITOL, V145, P245, DOI 10.1016/j.vetpar.2006.12.012
  20. OPS/OMS, 2013, US1, V1, P1
  21. Organization Panamericana de la Salud/Organizacion Mundial de la Salud, 2016, GUIA AB INT LEISHM P
  22. Pan American Health Organization, 2018, LEISHMANIASIS
  23. Luz ZMP, 2009, MEM I OSWALDO CRUZ, V104, P62, DOI 10.1590/S0074-02762009000100010
  24. Quintella LP, 2009, REV I MED TROP, V51, P83, DOI 10.1590/S0036-46652009000200005
  25. Ramirez JR, 2000, J CLIN MICROBIOL, V38, P3768
  26. Reed SG, 1996, CLIN DERMATOL, V14, P471, DOI 10.1016/0738-081X(96)00038-7
  27. Reithinger R, 2007, J CLIN MICROBIOL, V45, P21, DOI 10.1128/JCM.02029-06
  28. RIDLEY DS, 1983, J PATHOL, V141, P83, DOI 10.1002/path.1711410109
  29. Schubach A, 2001, MEM I OSWALDO CRUZ, V96, P987, DOI 10.1590/S0074-02762001000700018
  30. SHAW JJ, 1975, T ROY SOC TROP MED H, V69, P323, DOI 10.1016/0035-9203(75)90127-3
  31. SILVEIRA FT, 1991, T ROY SOC TROP MED H, V85, P735, DOI 10.1016/0035-9203(91)90437-4
  32. SOTTO MN, 1989, ACTA TROP, V46, P121, DOI 10.1016/0001-706X(89)90006-5
  33. Tresserra F., 2016, REV SENOL PATOL MAMA, V29, P26, DOI [10.1016/j.senol.2015.11.004, DOI 10.1016/J.SENOL.2015.11.004]
  34. Vergel C, 2005, AM J TROP MED HYG, V72, P423, DOI 10.4269/ajtmh.2005.72.423
  35. von Stebut E, 2007, EXP DERMATOL, V16, P340, DOI 10.1111/j.1600-0625.2007.00554.x
  36. XAVIER S. C., 2006, BMC VET RES, V2, P7, DOI 10.1186/1746-6148-2-17
  37. Yuil J. M. R., 2012, DERMATOLOGIA COSMET, V10, P13

Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - LIM/50