Effect of echium oil combined with phytosterols on biomarkers of atherosclerosis in LDLr-knockout mice: Echium oil is a potential alternative to marine oils for use in functional foods

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorBOTELHO, Patricia Borges
dc.contributor.authorGUIMARAES, Jessica Pereira
dc.contributor.authorMARIANO, Karina Rocha
dc.contributor.authorAFONSO, Milessa da Silva
dc.contributor.authorKOIKE, Marcia Kiyomi
dc.contributor.authorLOTTENBERG, Ana Maria Pita
dc.contributor.authorCASTRO, Inar Alves
dc.date.accessioned2015-12-10T16:52:46Z
dc.date.available2015-12-10T16:52:46Z
dc.date.issued2015
dc.description.abstractBioactive compounds may be an alternative approach to prevent atherosclerosis. To evaluate this hypothesis, LDLr-knockout mice were supplemented with omega-3 fatty acids from Echium oil (10.24mg/d of oil with 1,14mg/d of SDA and 9.06mg/d of ALA) equivalent to 0.7mg/d of EPA after conversion, combined or not with phytosterols (0.76mg/d), during the first 2 months of life. Subsequently, dyslipidaemia was induced by a high-fat diet for the following 2 months. Echium oil, isolated or combined with phytosterols, improved lipid profile in plasma reducing triacylglycerol (90.37.6mg/dL) and VLDL-c (18.01.5mg/dL) concentrations when compared with Control (115.8 +/- 9.4mg/dL and 23.2 +/- 1.9mg/dL, respectively). Echium oil also increased catalase (5.66 +/- 0.13U/mg protein) while Echium oil combined with phytosterol increased glutathione peroxidase activity (26.27 +/- 0.10U/mg protein) when compared with Control (5.18 +/- 0.10U/mg protein and 25.31 +/- 0.16U/mg protein, respectively). In addition, groups receiving Echium oil have reduced malondialdehyde concentration in liver (p=0.05). However, no difference was observed in fatty streak lesions when compared with Control. Isolated phytosterols did not change cholesterol absorption and increased lesion area compared with control group. This result can be associated with the high dose applied in the first step of supplementation and with the form of supplementation (gavage). Practical applications: One factor that contributes to the number of deaths from cardiovascular disease is that pharmacological interventions usually start too late in life. For this reason, functional foods development is a very important strategy to prevent atherosclerosis, since their inclusion in diet can start much earlier. However, it represents a challenge because many physiological responses from chronic consumption of bioactive compounds are still unknown. In this study, considering the positive results on triglyceridemia and oxidative stress biomarkers, we suggest that Echium oil can be an alternative for development of functional foods. We selected Echium oil due to its higher proportion of stearidonic fatty acids (pro-EPA) and lower sensory limitation than marine oils. Echium oil improves lipid profile and reduces oxidative stress, while phytosterol increases fatty streak.
dc.description.indexPubMed
dc.description.sponsorshipFAPESP [09/15649-7, 10/12042-1, 10/08225-3]
dc.identifier.citationEUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, v.117, n.10, p.1561-1568, 2015
dc.identifier.doi10.1002/ejlt.201500004
dc.identifier.eissn1438-9312
dc.identifier.issn1438-7697
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/12534
dc.language.isoeng
dc.publisherWILEY-BLACKWELL
dc.relation.ispartofEuropean Journal of Lipid Science and Technology
dc.rightsrestrictedAccess
dc.rights.holderCopyright WILEY-BLACKWELL
dc.subjectAtherosclerosis
dc.subjectEchium
dc.subjectMice
dc.subjectOmega 3
dc.subjectOxidative stress
dc.subjectPhytosterols
dc.subject.othercholesterol-metabolism
dc.subject.otherplant sterols
dc.subject.otherplasma
dc.subject.otheromega-3-fatty-acids
dc.subject.othermechanisms
dc.subject.otherdisease
dc.subject.otherstanols
dc.subject.otherlesions
dc.subject.otherliver
dc.subject.othergene
dc.subject.wosFood Science & Technology
dc.subject.wosNutrition & Dietetics
dc.titleEffect of echium oil combined with phytosterols on biomarkers of atherosclerosis in LDLr-knockout mice: Echium oil is a potential alternative to marine oils for use in functional foods
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalBOTELHO, Patricia Borges:Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, LADAF, BR-05508900 Sao Paulo, Brazil
hcfmusp.author.externalGUIMARAES, Jessica Pereira:Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, LADAF, BR-05508900 Sao Paulo, Brazil
hcfmusp.author.externalMARIANO, Karina Rocha:Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, LADAF, BR-05508900 Sao Paulo, Brazil
hcfmusp.author.externalCASTRO, Inar Alves:Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, LADAF, BR-05508900 Sao Paulo, Brazil
hcfmusp.citation.scopus15
hcfmusp.contributor.author-fmusphcMILESSA DA SILVA AFONSO
hcfmusp.contributor.author-fmusphcMARCIA KIYOMI KOIKE
hcfmusp.contributor.author-fmusphcANA MARIA PITA LOTTENBERG
hcfmusp.description.beginpage1561
hcfmusp.description.endpage1568
hcfmusp.description.issue10
hcfmusp.description.volume117
hcfmusp.origemWOS
hcfmusp.origem.scopus2-s2.0-84944165184
hcfmusp.origem.wosWOS:000362964000009
hcfmusp.publisher.cityHOBOKEN
hcfmusp.publisher.countryUSA
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