Salivary gland homogenates from wild-caught sand flies Lutzomyia flaviscutellata and Lutzomyia (Psychodopygus) complexus showed inhibitory effects on Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis infection in BALB/c mice

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art_FRANCESQUINI_Salivary_gland_homogenates_from_wildcaught_sand_flies_Lutzomyia_2014.PDF (1.49 MB)
Citações na Scopus
9
Tipo de produção
article
Data de publicação
2014
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
WILEY-BLACKWELL
Autores
SILVEIRA, Fernando T.
Citação
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, v.95, n.6, p.418-426, 2014
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
During the natural transmission of Leishmania parasites, the infected sand fly female regurgitates promastigotes into the host's skin together with its saliva. It has been reported that vector saliva contains immunomodulatory molecules that facilitate the establishment of infection. Thus, the main objective of this study was to evaluate the specificity of Lutzomyia (Lu.) flaviscutellata and Lu.(Psychodopygus) complexus salivas on the infectivity of Leishmania (L.) (Leishmania) amazonensis and L.(Viannia) braziliensis, respectively. BALB/c mice were inoculated into the skin of hind footpad with L.(L.) amazonensis and L.(V.) braziliensis promastigotes in the absence or presence of Lu.flaviscutellata and Lu.(P.) complexus salivary gland homogenates (SGHs). The evolution of the infection was evaluated by lesion size, histopathological analysis and determination of the parasite load in the skin biopsies collected from the site of infection at 4 and 8weeks PI. The lesion size and the parasite load of both groups of mice infected in the presence of SGHs were smaller than the control groups. The histopathological features showed that the inflammatory reaction was less prominent in the groups of mice infected in the presence of both SGHs when compared to the control group. The results showed that the presence of SGHs of Lu.flaviscutellata and Lu.(P.) complexus led to induction of processes that were disadvantageous to parasite establishment during infection by L.(L.) amazonensis and L.(V.) braziliensis. An inhibitory effect on Leishmania infection could be observed in both groups inoculated with SGHs, especially when the SGH from Lu.(P.) complexus was used.
Palavras-chave
BALB, c mice, experimental cutaneous leishmaniasis, Leishmania (Leishmania) amazonensis, Leishmania (Viannia) braziliensis, Lutzomyia (Psychodopygus) complexus, Lutzomyia flaviscutellata, salivary gland homogenate
URI
https://observatorio.fm.usp.br/handle/OPI/9096
Referências
  1. Andrade BB, 2007, SCAND J IMMUNOL, V66, P122, DOI 10.1111/j.1365-3083.2007.01964.x
  2. Belkaid Y, 1998, J EXP MED, V188, P1941, DOI 10.1084/jem.188.10.1941
  3. Carregaro V, 2013, BMC MICROBIOL, V13, DOI 10.1186/1471-2180-13-102
  4. Carvalho AK, 2012, PARASITE IMMUNOL, V34, P395, DOI 10.1111/j.1365-3024.2012.01370.x
  5. CHAMPAGNE DE, 1994, PARASITOL TODAY, V10, P430, DOI 10.1016/0169-4758(94)90173-2
  6. DeKrey GK, 1998, INFECT IMMUN, V66, P827
  7. desMoura T R., 2005, INFECT IMMUN, V73, P5827
  8. desMoura T R., 2013, PLOS NEGLECT TROP D, V7, pe2242
  9. Donnelly KB, 1998, J PARASITOL, V84, P97, DOI 10.2307/3284537
  10. Gomes R, 2008, P NATL ACAD SCI USA, V105, P7845, DOI 10.1073/pnas.0712153105
  11. HALL LR, 1995, J IMMUNOL, V155, P3501
  12. Kamhawi S, 2000, MICROBES INFECT, V2, P1765, DOI 10.1016/S1286-4579(00)01331-9
  13. Kamhawi S, 2000, SCIENCE, V290, P1351, DOI 10.1126/science.290.5495.1351
  14. Laurenti MD, 2009, PARASITOL INT, V58, P220, DOI 10.1016/j.parint.2009.05.005
  15. Laurenti MD, 2009, SCAND J IMMUNOL, V70, P389, DOI 10.1111/j.1365-3083.2009.02310.x
  16. LOCKSLEY RM, 1991, RES IMMUNOL, V142, P28, DOI 10.1016/0923-2494(91)90007-6
  17. Maioli TU, 2004, PARASITOL RES, V94, P207, DOI 10.1007/s00436-004-1193-6
  18. MILES MA, 1980, T ROY SOC TROP MED H, V74, P243, DOI 10.1016/0035-9203(80)90253-9
  19. Norsworthy NB, 2004, INFECT IMMUN, V72, P1240, DOI 10.1128/IAI.72.3.1240-1247.2004
  20. Oliveira F, 2008, PLOS NEGLECT TROP D, V2, DOI 10.1371/journal.pntd.0000226
  21. Paranhos-Silva M, 2003, VET PARASITOL, V114, P97, DOI [10.1016/S0304-4017(03)00132-8, 10.1016/S0304-4017(03)00261-9]
  22. Passero LFD, 2010, APMIS, V118, P973, DOI 10.1111/j.1600-0463.2010.02679.x
  23. Qi H, 2004, INFECT IMMUN, V72, P988, DOI 10.1128/IAI.72.2.988-995.2004
  24. RIBEIRO JMC, 1989, EXP PARASITOL, V69, P104, DOI 10.1016/0014-4894(89)90177-X
  25. RIBEIRO JMC, 1987, ANNU REV ENTOMOL, V32, P463, DOI 10.1146/annurev.ento.32.1.463
  26. Roberts M. T. M., 2006, BR MED B, V75-76, P115, DOI [10.1093/bmb/ldl003, DOI 10.1093/BMB/ID1003]
  27. Samuelson E., 1991, J EXP MED, V173, P49
  28. SHAW JJ, 1989, T ROY SOC TROP MED H, V83, P783, DOI 10.1016/0035-9203(89)90326-X
  29. THEODOS CM, 1993, PARASITE IMMUNOL, V15, P481, DOI 10.1111/j.1365-3024.1993.tb00634.x
  30. THEODOS CM, 1991, INFECT IMMUN, V59, P1592
  31. Thiakaki M, 2005, MICROBES INFECT, V7, P760, DOI 10.1016/j.micinf.2005.01.013
  32. TITUS RG, 1990, PARASITOL TODAY, V6, P157, DOI 10.1016/0169-4758(90)90338-5
  33. TITUS RG, 1988, SCIENCE, V239, P1306, DOI 10.1126/science.3344436
  34. Wikel SK, 1999, INT J PARASITOL, V29, P851, DOI 10.1016/S0020-7519(99)00042-9

Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - LIM/50