Comparing the phenotypic, genotypic, and proteomic identification of Trichosporon species: A globally emerging yeast of medical importance

Imagem de Miniatura
Arquivos
art_LARA_Comparing_the_phenotypic_genotypic_and_proteomic_identification_of_2021.PDF (534.17 KB)
Citações na Scopus
5
Tipo de produção
article
Data de publicação
2021
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
OXFORD UNIV PRESS
Autores
LARA, Bruna Rossini
CAMARGO, Bruno Braidotti de
PAULA, Claudete Rodrigues
LEITE JUNIOR, Diniz Pereira
GARCES, Hans Garcia
ARNONI, Mariana Volpe
SILVEIRA, Monica
TAKAHASHI, Juliana Possatto Fernandes
Citação
MEDICAL MYCOLOGY, v.59, n.12, p.1181-1190, 2021
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Trichosporon spp. are widely distributed in the nature, comprising species that inhabit different ecological niches and can be found in the water, soil, and body surface of animals and humans. Such microorganisms have been classically associated with superficial infections; however, in the last decades, they have also been related to disseminated infections in immunocompromised patients, behaving as opportunistic agents, which demands rapid and accurate species identification for efficient therapy. Concordance level between the traditional phenotypic method and the molecular technique (gold standard) in the identification of all 59 Trichosporon samples was 59.3%. Identification concordance between MALDI-TOF spectrometry and the molecular technique was 71.2%. No isolate of environmental origin was identifiable by MALDI-TOF mass spectrometry (MS), and 100% of such environmental isolates were discordant for IGS region sequencing and phenotypic characterization. Both comparisons evidenced greatest concordance in the identification of T. asahii. The species T. debeurmannianum, T. dermatis, T. venhuisii and T. insectorum were not properly identified by both MALDI-TOF MS and the phenotypic technique. MALDI-TOF MS, in particular, seems to be appropriate to investigate yeasts of the genus Trichosporon; however, database updates are still necessary, especially for species that are not common in the clinical routine. With the aim of helping understand the aspects involved in early and accurate diagnosis of infections caused by this opportunistic agent, the present study compared the phenotypic, molecular (IGS region) and mass-spectrometry (MALDI-TOF) identification of 59 yeasts of the genus Trichosporon which had clinical and environmental origin and were kept in a mycology collection.
Palavras-chave
Trichosporon spp., emerging pathogens, phenotypic identification, genotypic identification, MALDI-TOF MS
URI
https://observatorio.fm.usp.br/handle/OPI/44661
Referências
  1. Ahangarkani F, 2021, MYCOSES, V64, P918, DOI 10.1111/myc.13306
  2. Ahmad S, 2005, J MED MICROBIOL, V54, P639, DOI 10.1099/jmm.0.45972-0
  3. Alanio A, 2011, CLIN MICROBIOL INFEC, V17, P750, DOI 10.1111/j.1469-0691.2010.03323.x
  4. Almeida JN, 2014, TRANSPL INFECT DIS, V16, P135, DOI 10.1111/tid.12179
  5. Almeida JND, 2016, FRONT MICROBIOL, V7, DOI 10.3389/fmicb.2016.01629
  6. Castanho G, 2020, TRICHOSPORONOSIS
  7. Chagas-Neto TC, 2009, J CLIN MICROBIOL, V47, P1074, DOI 10.1128/JCM.01614-08
  8. Colombo AL, 2011, CLIN MICROBIOL REV, V24, P682, DOI 10.1128/CMR.00003-11
  9. Dabas Y, 2017, MED MYCOL, V55, P518, DOI 10.1093/mmy/myw100
  10. de Almeida AA, 2016, REV ARGENT MICROBIOL, V48, P62, DOI 10.1016/j.ram.2015.11.005
  11. De Carolis E, 2012, CLIN MICROBIOL INFEC, V18, P475, DOI 10.1111/j.1469-0691.2011.03599.x
  12. De Hoog G. S., 2000, ATLAS CLIN FUNGI
  13. Duarte-Oliveira C, 2017, FRONT CELL INFECT MI, V7, DOI 10.3389/fcimb.2017.00118
  14. Escarra F, 2017, ARCH ARGENT PEDIATR, V115, pE311, DOI 10.5546/aap.2017.e311
  15. Francisco EC, 2019, CLIN MICROBIOL INFEC, V25, DOI 10.1016/j.cmi.2019.03.026
  16. Galligan ER, 2019, J CUTAN PATHOL, V46, P159, DOI 10.1111/cup.13397
  17. Taverna CG, 2014, MED MYCOL, V52, P356, DOI 10.1093/mmy/myt029
  18. Gunn SR, 2006, J CLIN MICROBIOL, V44, P1175, DOI 10.1128/JCM.44.3.1175-1177.2006
  19. Hagen F, 2015, FUNGAL GENET BIOL, V78, P16, DOI 10.1016/j.fgb.2015.02.009
  20. Hazirolan G, 2018, J MYCOL MED, V28, P452, DOI 10.1016/j.mycmed.2018.06.006
  21. Iqbal N, 2020, CUREUS, V12, DOI 10.7759/cureus.8973
  22. Iturrieta-Gonzalez IA, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0109553
  23. Kolecka A, 2013, J CLIN MICROBIOL, V51, P2491, DOI 10.1128/JCM.00470-13
  24. Koneman EK, 1987, PRACTICAL LAB MYCOLO
  25. Kumar S, 2016, MOL BIOL EVOL, V33, P1870, DOI [10.1093/molbev/msv279, 10.1093/molbev/msw054]
  26. Kurtzman CP, 2011, YEASTS: A TAXONOMIC STUDY, VOLS 1-3, 5TH EDITION, P1
  27. Lara BR, 2019, MYCOLOGIA, V111, P793, DOI 10.1080/00275514.2019.1637645
  28. Lima-Neto R, 2014, BRAZ J MICROBIOL, V45, P515, DOI [10.1590/S1517-83822014005000044, 10.1590/S1517-83822014000200020]
  29. Liu XZ, 2015, STUD MYCOL, P85, DOI 10.1016/j.simyco.2015.12.001
  30. Martins-Diniz JN, 2005, REV SAUDE PUBL, V39, P398, DOI 10.1590/S0034-89102005000300010
  31. Milan EP, 2018, BMC INFECT DIS, V18, DOI 10.1186/s12879-018-3363-7
  32. Monzani V, 2020, J MYCOL MED, V30, DOI 10.1016/j.mycmed.2019.100916
  33. Nath R, 2018, MYCOPATHOLOGIA, V183, P585, DOI 10.1007/s11046-017-0231-8
  34. Pagnocca FC, 2010, INT J SYST EVOL MICR, V60, P1454, DOI 10.1099/ijs.0.015727-0
  35. Pote ST, 2020, INFECT DRUG RESIST, V13, P1133, DOI 10.2147/IDR.S238002
  36. Ramirez I, 2020, MYCOPATHOLOGIA, V185, P377, DOI 10.1007/s11046-019-00416-w
  37. Rodriguez-Tudela JL, 2005, ANTIMICROB AGENTS CH, V49, P4026, DOI 10.1128/AAC.49.10.4026-4034.2005
  38. Sah R, 2019, IMMUN INFLAMM DIS, V7, P125, DOI 10.1002/iid3.250
  39. Santos C, 2010, J APPL MICROBIOL, V108, P375, DOI 10.1111/j.1365-2672.2009.04448.x
  40. Sugita T, 2002, J CLIN MICROBIOL, V40, P1826, DOI 10.1128/JCM.40.5.1826-1830.2002
  41. Suh SO, 2015, J MED MICROBIOL, V64, P1135, DOI 10.1099/jmm.0.000134
  42. Taj-Aldeen SJ, 2009, J CLIN MICROBIOL, V47, P1791, DOI 10.1128/JCM.02222-08
  43. Santo EPTD, 2020, MYCOPATHOLOGIA, V185, P307, DOI 10.1007/s11046-019-00407-x
  44. Wang DX, 2016, ANAL CHEM, V88, P6867, DOI 10.1021/acs.analchem.6b01486
  45. Zhou P, 2020, NATURE, V588, pE6, DOI [10.1038/s41586-020-2951-z, 10.1038/s41586-020-2012-7]

Coleções
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/53