Evaluation of real-time PCR assay to detect Schistosoma mansoni infections in a low endemic setting

Página do item simplificado
dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorESPIRITO-SANTO, Maria Cristina Carvalho
dc.contributor.authorALVARADO-MORA, Monica Viviana
dc.contributor.authorDIAS-NETO, Emmanuel
dc.contributor.authorBOTELHO-LIMA, Livia Souza
dc.contributor.authorMOREIRA, Joao Paulo
dc.contributor.authorAMORIM, Maria
dc.contributor.authorPINTO, Pedro Luiz Silva
dc.contributor.authorHEATH, Ashley R.
dc.contributor.authorCASTILHO, Vera Lucia Pagliusi
dc.contributor.authorGONCALVES, Elenice Messias do Nascimento
dc.contributor.authorLUNA, Expedito Jose de Albuquerque
dc.contributor.authorCARRILHO, Flair Jose
dc.contributor.authorPINHO, Joao Renato Rebello
dc.contributor.authorGRYSCHEK, Ronaldo Cesar Borges
dc.date.accessioned2015-02-06T20:12:05Z
dc.date.available2015-02-06T20:12:05Z
dc.date.issued2014
dc.description.abstractBackground: Schistosomiasis constitutes a major public health problem, and 200 million people are estimated to be infected with schistosomiasis worldwide. In Brazil, schistosomiasis has been reported in 19 states, showing areas of high and medium endemicity and a wide range of areas of low endemicity (ALE). Barra Mansa in Rio de Janeiro state has an estimated prevalence of 1%. ALE represent a new challenge for the helminth control because about 75% of infected individuals are asymptomatic and infections occur with a low parasite load (<100 eggs per gram of feces), causing a decrease in sensitivity of stool parasitological techniques, which are a reference for the laboratory diagnosis of this helminth. The objective of this study was to evaluate the performance of a TaqMan quantitative polymerase chain reaction (qPCR) technique in serum and feces DNA samples using the techniques of Kato-Katz (KK), Hoffman, Pons and Janer (HH) as references, during an epidemiological survey using fecal samples and sera from randomized residents from an ALE. Methods: A cross-sectional study conducted from April to December 2011 using a probabilistic sampling that collected 572 fecal and serum samples. The laboratory diagnostic techniques used were: KK, HH and qPCR ( feces and serum). Results: We obtained the following results using the different diagnostic techniques: KK and HH, 0.9% (n = 5); qPCR-feces, 9.6% (n = 55); and qPCR-serum, 1.4% (n = 8). The qPCR-feces presented the highest positivity, whereas the techniques of HH and KK were the least sensitive to detect infections (0.8%). Compared to HH and KK, qPCR-feces showed a statistically significant difference in positivity (p < 0.05), although with poor agreement. Conclusion: The positivity rate presented by the qPCR approach was far higher than that obtained by parasitological techniques. The lack of adequate surveillance in ALE of schistosomiasis indicates a high possibility of these areas being actually of medium and high endemicity. This study presents a control perspective, pointing to the possibility of using combined laboratory tools in the diagnosis of schistosomiasis in ALE.
dc.description.indexMEDLINE
dc.description.sponsorshipSao Paulo Research Foundation [07/53457-7, 08/50461-6, 10/52615-0]
dc.identifier.citationBMC INFECTIOUS DISEASES, v.14, article ID 558, 10p, 2014
dc.identifier.doi10.1186/s12879-014-0558-4
dc.identifier.issn1471-2334
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/8845
dc.language.isoeng
dc.publisherBIOMED CENTRAL LTD
dc.relation.ispartofBMC Infectious Diseases
dc.rightsopenAccess
dc.rights.holderCopyright BIOMED CENTRAL LTD
dc.subjectSchistosomiasis mansoni
dc.subjectTaqMan (R) Real-Time PCR system
dc.subjectLaboratory diagnosis
dc.subjectLow endemicity areas
dc.subjectBrazil/epidemiology
dc.subject.otherpolymerase-chain-reaction
dc.subject.otherribosomal-rna genes
dc.subject.otherkato-katz
dc.subject.otherstool samples
dc.subject.otherlow transmission
dc.subject.otherdna
dc.subject.otherdiagnosis
dc.subject.otherintensity
dc.subject.otherareas
dc.subject.othersensitivity
dc.subject.wosInfectious Diseases
dc.titleEvaluation of real-time PCR assay to detect Schistosoma mansoni infections in a low endemic setting
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.affiliation.countryEstados Unidos
hcfmusp.affiliation.countryisous
hcfmusp.author.externalALVARADO-MORA, Monica Viviana:Univ Sao Paulo, Sch Med, Dept Gastroenterol, Lab Trop Gastroenterol & Hepatol, Sao Paulo, Brazil
hcfmusp.author.externalAMORIM, Maria:AC Camargo Canc Ctr, Lab Med Genom, Sao Paulo, Brazil
hcfmusp.author.externalPINTO, Pedro Luiz Silva:Adolfo Lutz Inst, Parasitol & Mycol Serv, Dept Enteroparasites, Sao Paulo, Brazil
hcfmusp.author.externalHEATH, Ashley R.:Sigma Custom Prod, The Woodlands, TX 77380 USA
hcfmusp.citation.scopus34
hcfmusp.contributor.author-fmusphcMARIA CRISTINA CARVALHO DO ESPIRITO SANTO
hcfmusp.contributor.author-fmusphcEMMANUEL DIAS NETO
hcfmusp.contributor.author-fmusphcLIVIA DE SOUZA BOTELHO LIMA
hcfmusp.contributor.author-fmusphcJOAO PAULO MOREIRA
hcfmusp.contributor.author-fmusphcVERA LUCIA PAGLIUSI CASTILHO
hcfmusp.contributor.author-fmusphcELENICE MESSIAS DO NASCIMENTO GONCALVES
hcfmusp.contributor.author-fmusphcEXPEDITO JOSE DE ALBUQUERQUE LUNA
hcfmusp.contributor.author-fmusphcFLAIR JOSE CARRILHO
hcfmusp.contributor.author-fmusphcJOAO RENATO REBELLO PINHO
hcfmusp.contributor.author-fmusphcRONALDO CESAR BORGES GRYSCHEK
hcfmusp.description.articlenumber558
hcfmusp.description.volume14
hcfmusp.origemWOS
hcfmusp.origem.scopus2-s2.0-84929507684
hcfmusp.origem.wosWOS:000343831200001
hcfmusp.publisher.cityLONDON
hcfmusp.publisher.countryENGLAND
hcfmusp.relation.reference[Anonymous], 2008, SINANNET07052008
hcfmusp.relation.referenceBergquist NR, 2002, TRENDS PARASITOL, V18, P309, DOI 10.1016/S1471-4922(02)02301-2
hcfmusp.relation.referenceBrasil. Ministerio da Saude, 1976, CONS DES SOC
hcfmusp.relation.referenceBrasil. Ministerio da Saude. Secretaria de Vigilancia a Saude: Sistema de Informacao do Programa de Controle da Esquistossomose, 2012, SIST INF PROGR CONTR
hcfmusp.relation.referenceCarneiro TR, 2012, REV SOC BRAS MED TRO, V45, P510, DOI 10.1590/S0037-86822012000400019
hcfmusp.relation.referenceCavalcanti MG, 2013, TRENDS PARASITOL, V29, P75, DOI 10.1016/j.pt.2012.11.003
hcfmusp.relation.referenceChitsulo L, 2000, ACTA TROP, V77, P41, DOI 10.1016/S0001-706X(00)00122-4
hcfmusp.relation.referenceCHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1006/abio.1987.9999
hcfmusp.relation.referenceCnops L, 2012, TROP MED INT HEALTH, V17, P1208, DOI 10.1111/j.1365-3156.2012.03060.x
hcfmusp.relation.referencede Janeiro R, 2009, SECRETARIA ESTADO SA
hcfmusp.relation.referencede Noya BA, 2006, MEM I OSWALDO CRUZ, V101, P29, DOI 10.1590/S0074-02762006000900006
hcfmusp.relation.referenceDoenhoff MJ, 2004, TRENDS PARASITOL, V20, P35, DOI 10.1016/j.pt.2003.10.019
hcfmusp.relation.referenceEbrahim A, 1997, AM J TROP MED HYG, V57, P706
hcfmusp.relation.referenceEngels D, 1996, AM J TROP MED HYG, V54, P319
hcfmusp.relation.referenceEnk MJ, 2008, ACTA TROP, V108, P222, DOI 10.1016/j.actatropica.2008.09.016
hcfmusp.relation.referenceEnk MJ, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0038947
hcfmusp.relation.referenceEspírito-Santo Maria Cristina Carvalho do, 2012, Rev Inst Med Trop Sao Paulo, V54, P245
hcfmusp.relation.referenceEspirito-Santo MCC, 2011, 47 CONGR SOC BRAS ME, V85, P41
hcfmusp.relation.referenceEspirito-Santo MCC, 2012, 18 INT C TROP MED MA, VII, P662
hcfmusp.relation.referenceEspirito-Santo MCC, 2014, EXP PARASITOL, V143, P83, DOI 10.1016/j.exppara.2014.05.013
hcfmusp.relation.referenceGomes ALD, 2006, MEM I OSWALDO CRUZ, V101, P133, DOI 10.1590/S0074-02762006000900021
hcfmusp.relation.referenceGomes LI, 2009, MEM I OSWALDO CRUZ, V104, P1194, DOI 10.1590/S0074-02762009000800021
hcfmusp.relation.referenceHAMBURGER J, 1991, MOL BIOCHEM PARASIT, V44, P73, DOI 10.1016/0166-6851(91)90222-R
hcfmusp.relation.referenceKato K, 1954, JAPAN J PARASITOL, V3, P35
hcfmusp.relation.referenceKatz N, 1972, Rev Inst Med Trop Sao Paulo, V14, P397
hcfmusp.relation.referenceKirkwood BR, 2003, ESSENTIAL MED STAT
hcfmusp.relation.referenceOliveira LMA, 2010, DIAGN MICR INFEC DIS, V68, P416, DOI 10.1016/j.diagmicrobio.2010.07.016
hcfmusp.relation.referencePontes LA, 2003, AM J TROP MED HYG, V68, P652
hcfmusp.relation.referencePontes LA, 2002, AM J TROP MED HYG, V66, P157
hcfmusp.relation.referencePordeus LC, 2008, EPIDEMIOL SERV SAUDE, V17, P163
hcfmusp.relation.referenceRUIZTIBEN E, 1979, AM J TROP MED HYG, V28, P230
hcfmusp.relation.referenceSAIKI RK, 1988, SCIENCE, V239, P487, DOI 10.1126/science.2448875
hcfmusp.relation.referenceSIMPSON AJG, 1984, EUR J BIOCHEM, V139, P41, DOI 10.1111/j.1432-1033.1984.tb07973.x
hcfmusp.relation.referenceSorgho H, 2005, ACTA TROP, V93, P169, DOI 10.1016/j.actatropica.2004.10.006
hcfmusp.relation.referenceSPOTILA LD, 1991, MOL BIOCHEM PARASIT, V48, P117, DOI 10.1016/0166-6851(91)90172-3
hcfmusp.relation.referenceTEESDALE CH, 1985, T ROY SOC TROP MED H, V79, P369, DOI 10.1016/0035-9203(85)90384-0
hcfmusp.relation.referenceTeles HMS, 2001, THESIS U ESTADUAL CA
hcfmusp.relation.referenceten Hove RJ, 2008, T ROY SOC TROP MED H, V102, P179, DOI 10.1016/j.trstmh.2007.10.011
hcfmusp.relation.referenceVANKEULEN H, 1985, MOL BIOCHEM PARASIT, V15, P215, DOI 10.1016/0166-6851(85)90121-5
hcfmusp.relation.referenceWHO, 2000, REP INF CONS SCHIST
hcfmusp.relation.referenceWichmann D, 2009, PLOS NEGLECT TROP D, V3, DOI 10.1371/journal.pntd.0000422
hcfmusp.relation.referenceWichmann D, 2013, BMC INFECT DIS, V13, DOI 10.1186/1471-2334-13-55
hcfmusp.relation.referenceZhang YY, 2009, ACTA TROP, V112, P16, DOI 10.1016/j.actatropica.2009.05.020
hcfmusp.relation.referenceZhou L, 2011, ACTA TROP, V120, P88, DOI 10.1016/j.actatropica.2011.06.006
hcfmusp.scopus.lastupdate2024-05-10
relation.isAuthorOfPublication85a56a81-0c01-4e0e-b028-7ce148134888
relation.isAuthorOfPublication1f62ca41-9e7d-43b8-b33c-51728f19f7a1
relation.isAuthorOfPublication3cb54390-a0a9-4eaa-875e-7344854cfc4f
relation.isAuthorOfPublicationfae9e4a0-1460-4a57-932f-9329a8eae667
relation.isAuthorOfPublicatione803c973-69d9-4d3a-8e7a-dc3aa9c9224a
relation.isAuthorOfPublicatione729f0ed-1d3d-4f4a-9cf8-a0bc527b7e40
relation.isAuthorOfPublication68ca9ace-34dc-4608-8ffa-b42f5278e34e
relation.isAuthorOfPublication9221b8d6-ad80-46e5-a56f-fb3b28a8c155
relation.isAuthorOfPublication0ada2eb1-5ac2-4e40-b4e2-6af14f33268b
relation.isAuthorOfPublication7ec8d577-b8db-4d87-b541-6def85d667e6
relation.isAuthorOfPublication.latestForDiscovery85a56a81-0c01-4e0e-b028-7ce148134888
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
art_ESPIRITO-SANTO_Evaluation_of_realtime_PCR_assay_to_detect_Schistosoma_2014.PDF
Tamanho:
582.04 KB
Formato:
Adobe Portable Document Format
Descrição:
publishedVersion (English)
Baixar
Coleções
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - FM/MFT
Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/03
Artigos e Materiais de Revistas Científicas - LIM/06
Carregar mais