Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorPEREIRA, Andreia Vieira
dc.contributor.authorBARROS, Gustavo de
dc.contributor.authorPINTO, Erika Gracielle
dc.contributor.authorTEMPONE, Andre Gustavo
dc.contributor.authorORSI, Ricardo de Oliveira
dc.contributor.authorSANTOS, Lucilene Delazari dos
dc.contributor.authorCALVI, Sueli
dc.contributor.authorFERREIRA JR., Rui Seabra
dc.contributor.authorPIMENTA, Daniel Carvalho
dc.contributor.authorBARRAVIERA, Benedito
dc.date.accessioned2016-03-14T14:51:02Z
dc.date.available2016-03-14T14:51:02Z
dc.date.issued2016
dc.description.abstractBackground: Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases. Methods: Africanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages was evaluated through mitochondrial oxidative activity. The production of anti-and pro-inflammatory cytokines, NO and H2O2 by macrophages was determined. Results: Promastigotes and intracellular amastigotes were susceptible to melittin (IC50 28.3 mu g.mL(-1) and 1.4 mu g.mL(-1), respectively), but also showed mammalian cell cytotoxicity with an IC50 value of 5.7 mu g.mL(-1). Uninfected macrophages treated with melittin increased the production of IL-10, TNF-alpha, NO and H2O2. Infected melittin-treated macrophages increased IL-12 production, but decreased the levels of IL-10, TNF-alpha, NO and H2O2. Conclusions: The results showed that melittin acts in vitro against promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Furthermore, they can act indirectly on intracellular amastigotes through a macrophage immunomodulatory effect.
dc.description.indexPubMed
dc.identifier.citationJOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES, v.22, article ID 1, 8p, 2016
dc.identifier.doi10.1186/s40409-016-0055-x
dc.identifier.issn1678-9199
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/13635
dc.language.isoeng
dc.publisherBIOMED CENTRAL LTD
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.rightsopenAccess
dc.rights.holderCopyright BIOMED CENTRAL LTD
dc.subjectMelittin
dc.subjectApis mellifera
dc.subjectLeishmania
dc.subjectLeishmaniasis
dc.subjectPeptides
dc.subjectToxins
dc.subjectAntiparasitic
dc.subjectCytokines
dc.subject.otherapis-mellifera venom
dc.subject.otherantimicrobial peptides
dc.subject.othernitric-oxide
dc.subject.otherphospholipase a(2)
dc.subject.othertrypanosoma-cruzi
dc.subject.otherhybrid peptide
dc.subject.otherbee venom
dc.subject.otherchagasi
dc.subject.othermacrophages
dc.subject.othersuperoxide
dc.subject.wosToxicology
dc.subject.wosTropical Medicine
dc.subject.wosZoology
dc.titleMelittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalPEREIRA, Andreia Vieira:Sao Paulo State Univ UNESP Univ Estadual Paulista, Botucatu Med Sch, Grad Program Trop Dis, BR-18610307 Botucatu, SP, Brazil
hcfmusp.author.externalBARROS, Gustavo de:Sao Paulo State Univ UNESP Univ Estadual Paulista, Botucatu Med Sch, Grad Program Trop Dis, BR-18610307 Botucatu, SP, Brazil
hcfmusp.author.externalTEMPONE, Andre Gustavo:Adolfo Lutz Inst, Dept Parasitol, Sao Paulo, SP, Brazil
hcfmusp.author.externalORSI, Ricardo de Oliveira:Sao Paulo State Univ UNESP Univ Estadual Paulista, Dept Anim Prod, Sch Vet Med & Anim Husb, BR-18610307 Botucatu, SP, Brazil; Sao Paulo State Univ UNESP Univ Estadual Paulista, Ctr Study Venoms & Venomous Anim CEVAP, BR-18610307 Botucatu, SP, Brazil
hcfmusp.author.externalSANTOS, Lucilene Delazari dos:Sao Paulo State Univ UNESP Univ Estadual Paulista, Botucatu Med Sch, Grad Program Trop Dis, BR-18610307 Botucatu, SP, Brazil; Sao Paulo State Univ UNESP Univ Estadual Paulista, Ctr Study Venoms & Venomous Anim CEVAP, BR-18610307 Botucatu, SP, Brazil
hcfmusp.author.externalCALVI, Sueli:Sao Paulo State Univ UNESP Univ Estadual Paulista, Botucatu Med Sch, Grad Program Trop Dis, BR-18610307 Botucatu, SP, Brazil; Sao Paulo State Univ UNESP Univ Estadual Paulista, Ctr Study Venoms & Venomous Anim CEVAP, BR-18610307 Botucatu, SP, Brazil
hcfmusp.author.externalFERREIRA JR., Rui Seabra:Sao Paulo State Univ UNESP Univ Estadual Paulista, Botucatu Med Sch, Grad Program Trop Dis, BR-18610307 Botucatu, SP, Brazil; Sao Paulo State Univ UNESP Univ Estadual Paulista, Ctr Study Venoms & Venomous Anim CEVAP, BR-18610307 Botucatu, SP, Brazil
hcfmusp.author.externalPIMENTA, Daniel Carvalho:Butantan Inst, Lab Biochem & Biophys, Sao Paulo, SP, Brazil
hcfmusp.author.externalBARRAVIERA, Benedito:Sao Paulo State Univ UNESP Univ Estadual Paulista, Botucatu Med Sch, Grad Program Trop Dis, BR-18610307 Botucatu, SP, Brazil; Sao Paulo State Univ UNESP Univ Estadual Paulista, Ctr Study Venoms & Venomous Anim CEVAP, BR-18610307 Botucatu, SP, Brazil
hcfmusp.citation.scopus24
hcfmusp.contributor.author-fmusphcERIKA GRACIELLE PINTO
hcfmusp.description.articlenumber1
hcfmusp.description.volume22
hcfmusp.origemWOS
hcfmusp.origem.pubmed26752985
hcfmusp.origem.scieloSCIELO:S1678-91992016000100301
hcfmusp.origem.scopus2-s2.0-84953405951
hcfmusp.origem.wosWOS:000367782800001
hcfmusp.publisher.cityLONDON
hcfmusp.publisher.countryENGLAND
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