A sensitive and reliable quantitative immunohistochemistry technique to evaluate the percentage of Trypanosoma cruzi-infected tissue area

Página do item simplificado
dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorFERREIRA-FILHO, Julio Cesar Rente
dc.contributor.authorBRAZ, Lucia Maria Almeida
dc.contributor.authorANDRINO, Marcos Luiz Alves
dc.contributor.authorYAMAMOTO, Lidia
dc.contributor.authorKANASHIRO, Edite Hatsumi Yamashiro
dc.contributor.authorSILVA, Ana Maria Goncalves da
dc.contributor.authorKANUNFRE, Kelly Aparecida
dc.contributor.authorOKAY, Thelma Suely
dc.date.accessioned2021-02-18T13:40:49Z
dc.date.available2021-02-18T13:40:49Z
dc.date.issued2021
dc.description.abstractQuantification of parasites in the context of Chagas disease is required to monitor the treatment with benznidazole, disease-associated cardiomyopathies and graft rejection after heart transplantation. As parasitological exams lack sensitivity, Real Time Polymerase Chain Reaction (rt-PCR) has emerged to evaluate the parasite load in blood samples and cardiac biopsies. However, despite its higher sensitivity, rt-PCR does not provide information on the location and distribution of amastigote nests within infected tissues, the characterization of inflammatory infiltrates or changes to tissue architecture. On the contrary, a sensitive immunohistochemistry technique (IHC) could fill these gaps. In the present study, a quantitative IHC exam was standardized and validated by testing adipose and cardiac tissues of experimentally infected mice containing variable parasite load levels of T. cruzi assessed by a sensitive Sybr Green rt-PCR with kDNA primers. Tissues were divided into four groups according to the parasite load: group A100 parasites/50 ng of DNA; group B-10 parasites; group C - around 1 parasite and group D less than 1 parasite/50 ng/DNA. IHC was able to detect T. cruzi in the four groups, even in group D tissues containing fractions of a single parasite/50 ng of DNA sample according to rt-PCR. In conclusion, a highly sensitivity and reliable quantitative immunohistochemistry technique was developed and is proposed to estimate the percentage of T. cruzi-infected tissue area in chagasic patients presenting with cardiomyopathies, as a complementary test to rt-PCR.eng
dc.description.indexMEDLINEeng
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES)CAPES [001]
dc.description.sponsorshipFAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2017/26543-1]
dc.identifier.citationPARASITOLOGY INTERNATIONAL, v.80, article ID 102210, 5p, 2021
dc.identifier.doi10.1016/j.parint.2020.102210
dc.identifier.eissn1873-0329
dc.identifier.issn1383-5769
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/39308
dc.language.isoeng
dc.publisherELSEVIER IRELAND LTDeng
dc.relation.ispartofParasitology International
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright ELSEVIER IRELAND LTDeng
dc.subjectCardiomyopathyeng
dc.subjectCardiac biopsieseng
dc.subjectEndomyocardial biopsieseng
dc.subjectAnti-T. cruzi antibodieseng
dc.subject.otherchagas-diseaseeng
dc.subject.otherparasitismeng
dc.subject.wosParasitologyeng
dc.titleA sensitive and reliable quantitative immunohistochemistry technique to evaluate the percentage of Trypanosoma cruzi-infected tissue areaeng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.author.externalANDRINO, Marcos Luiz Alves:Univ Sao Paulo, Fac Med, Inst Med Trop, Lab Soroepidemiol & Imunobiol, Sao Paulo, Brazil
hcfmusp.citation.scopus1
hcfmusp.contributor.author-fmusphcJULIO CESAR RENTE FERREIRA FILHO
hcfmusp.contributor.author-fmusphcLUCIA MARIA ALMEIDA BRAZ
hcfmusp.contributor.author-fmusphcLIDIA YAMAMOTO
hcfmusp.contributor.author-fmusphcEDITE HATSUMI YAMASHIRO KANASHIRO
hcfmusp.contributor.author-fmusphcANA MARIA GONCALVES DA SILVA
hcfmusp.contributor.author-fmusphcKELLY APARECIDA KANUNFRE
hcfmusp.contributor.author-fmusphcTHELMA SUELY OKAY
hcfmusp.description.articlenumber102210
hcfmusp.description.volume80
hcfmusp.origemWOS
hcfmusp.origem.pubmed33148532
hcfmusp.origem.scopus2-s2.0-85094968646
hcfmusp.origem.wosWOS:000596466200002
hcfmusp.publisher.cityCLAREeng
hcfmusp.publisher.countryIRELANDeng
hcfmusp.relation.referenceBilate AM, 2008, J INFECT DIS, V198, P614, DOI 10.1086/590347eng
hcfmusp.relation.referenceCaldas S, 2012, ACTA TROP, V123, P170, DOI 10.1016/j.actatropica.2012.05.002eng
hcfmusp.relation.referenceCaliari MV, 2002, VIRCHOWS ARCH, V441, P69, DOI 10.1007/s00428-001-0542-4eng
hcfmusp.relation.referenceCobo ED, 2012, J TROP MED-US, V2012, DOI 10.1155/2012/232646eng
hcfmusp.relation.referenceEzyaguirre E.J., 2011, DIAGNOSTIC IMMUNOHIS, P58, DOI [10.1016/B978-1-4160-5766-6.00007-8, DOI 10.1016/B978-1-4160-5766-6.00007-8]eng
hcfmusp.relation.referenceGreenfield E.A., 2014, ANTIBODIES LAB MANUA, P2014eng
hcfmusp.relation.referenceGuarner J, 2001, AM J TROP MED HYG, V65, P152, DOI 10.4269/ajtmh.2001.65.152eng
hcfmusp.relation.referenceHIGUCHI MD, 1993, CARDIOVASC PATHOL, V2, P101, DOI 10.1016/1054-8807(93)90021-Seng
hcfmusp.relation.referenceLewis MD, 2016, TRENDS PARASITOL, V32, P899, DOI 10.1016/j.pt.2016.08.009eng
hcfmusp.relation.referenceDias JCP, 2016, REV SOC BRAS MED TRO, V49, P3, DOI 10.1590/0037-8682-0505-2016eng
hcfmusp.relation.referencePortela-Lindoso AAB, 2003, REV SAUDE PUBL, V37, P107, DOI 10.1590/S0034-89102003000100016eng
hcfmusp.relation.referenceAzevedo PHR, 2018, REV SOC BRAS MED TRO, V51, P467, DOI 10.1590/0037-8682-0011-2018eng
hcfmusp.relation.referenceFerreira JCR, 2019, EXP PARASITOL, V200, P13, DOI 10.1016/j.exppara.2019.03.007eng
hcfmusp.relation.referenceSantoro F, 1987, Rev Soc Bras Med Trop, V20, P91, DOI 10.1590/S0037-86821987000200005eng
hcfmusp.relation.referenceSimões Marcus Vinicius, 2018, Int. J. Cardiovasc. Sci., V31, P173, DOI 10.5935/2359-4802.20180011eng
hcfmusp.relation.referenceYOUNESCHENNOUFI AB, 1988, T ROY SOC TROP MED H, V82, P77, DOI 10.1016/0035-9203(88)90269-6eng
hcfmusp.scopus.lastupdate2024-06-09
relation.isAuthorOfPublicationbf3eb57e-2854-4f3d-a358-53eeb77422a1
relation.isAuthorOfPublication4555c326-bdfa-4f27-9d80-f93036c9c13e
relation.isAuthorOfPublicationa38bf500-fc7d-4398-ba8a-3c207001ac97
relation.isAuthorOfPublication97e31d9f-c617-4015-9fae-563bb7c5ed04
relation.isAuthorOfPublication477eefc3-d54a-4be4-bd40-5da2fabe25ce
relation.isAuthorOfPublication62e60a57-e9d1-4354-b9a9-bbdd6da549d0
relation.isAuthorOfPublication6cda0b34-04dd-4679-8fb3-faa904acb401
relation.isAuthorOfPublication.latestForDiscoverybf3eb57e-2854-4f3d-a358-53eeb77422a1
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
art_FERREIRA-FILHO_A_sensitive_and_reliable_quantitative_immunohistochemistry_technique_to_2021.PDF
Tamanho:
1.37 MB
Formato:
Adobe Portable Document Format
Descrição:
publishedVersion (English)
Baixar
Coleções
Artigos e Materiais de Revistas Científicas - FM/MPE
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/06
Artigos e Materiais de Revistas Científicas - LIM/17
Artigos e Materiais de Revistas Científicas - LIM/38
Artigos e Materiais de Revistas Científicas - LIM/48