Optimization of von Willebrand factor multimer analysis in vertical mini-gel electrophoresis systems: A rapid procedure

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorTHOMAZINI, Camila Martos
dc.contributor.authorSOARES, Rosangela de Paula Silva
dc.contributor.authorROCHA, Tania Rubia Flores da
dc.contributor.authorSACHETTO, Ana Teresa Azevedo
dc.contributor.authorSANTORO, Marcelo Larami
dc.date.accessioned2019-03-26T14:42:25Z
dc.date.available2019-03-26T14:42:25Z
dc.date.issued2019
dc.description.abstractVon Willebrand disease (VWD) is a common cause of bleeding worldwide. Analysis of von Willebrand factor (VWF) multimer distribution (VWF:MD) is essential to properly classify and treat different types of VWD, and it is performed using a SDS agarose gel electrophoresis followed by Western blotting, a handmade technique that demands days to be completed and requires skillful execution. Aiming both to facilitate gel production and to shorten the preparation time, we developed an uncomplicated technique to provide agility in the analysis of VWF:MD, so that it can be easily accomplished in the routine practice of hemostasis laboratories. On that account, we used a commercial vertical mini-gel electrophoresis system for SDS-PAGE and a semi-dry transfer system, which allowed us to analyze VWF:MD of various samples in a period shorter than 12 h. This technique differentiated VWF:MD in human and animal plasmas under normal, congenital and acquired (experimental envenomation by Bothrops jararaca snake) conditions. This optimized method is cheap, rapid, reproducible, easy to be performed, and uses electrophoresis and Western blotting systems available in most laboratories. All these advantages encourage hemostasis professionals to use it in their routine practices. In order to facilitate the setup and accomplishment of the whole procedure step by step, videos were appended to the article.eng
dc.description.indexMEDLINEeng
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP) [2013/25177-0, 2013/20239-8]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [305245/2015-5]
dc.description.sponsorshipFundacao Butantan
dc.identifier.citationTHROMBOSIS RESEARCH, v.175, p.76-83, 2019
dc.identifier.doi10.1016/j.thromres.2019.01.018
dc.identifier.issn0049-3848
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/31256
dc.language.isoeng
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDeng
dc.relation.ispartofThrombosis Research
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright PERGAMON-ELSEVIER SCIENCE LTDeng
dc.subjectADAMTS13eng
dc.subjectBleedingeng
dc.subjectDiagnosiseng
dc.subjectElectrophoresiseng
dc.subjectProteolysiseng
dc.subject.othervonwillebrand-factoreng
dc.subject.othervenom metalloproteinaseseng
dc.subject.othertriplet structureeng
dc.subject.otherdisulfide bondseng
dc.subject.otherglycoprotein-ibeng
dc.subject.otherdiseaseeng
dc.subject.otherplasmaeng
dc.subject.otherclassificationeng
dc.subject.otherdiagnosiseng
dc.subject.otherthrombocytopeniaeng
dc.subject.wosHematologyeng
dc.subject.wosPeripheral Vascular Diseaseeng
dc.titleOptimization of von Willebrand factor multimer analysis in vertical mini-gel electrophoresis systems: A rapid procedureeng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.citation.scopus8
hcfmusp.contributor.author-fmusphcCAMILA MARTOS THOMAZINI
hcfmusp.contributor.author-fmusphcROSANGELA DE PAULA SILVA SOARES
hcfmusp.contributor.author-fmusphcTANIA RUBIA FLORES DA ROCHA
hcfmusp.contributor.author-fmusphcANA TERESA AZEVEDO SACHETTO
hcfmusp.contributor.author-fmusphcMARCELO LARAMI SANTORO
hcfmusp.description.beginpage76
hcfmusp.description.endpage83
hcfmusp.description.volume175
hcfmusp.origemWOS
hcfmusp.origem.pubmed30731387
hcfmusp.origem.scopus2-s2.0-85060920911
hcfmusp.origem.wosWOS:000458802600014
hcfmusp.publisher.cityOXFORDeng
hcfmusp.publisher.countryENGLANDeng
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