Assessment and comparison of bacterial load levels determined by quantitative amplifications in blood culture-positive and negative neonatal sepsis

Página do item simplificado
dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorSTRANIERI, Ines
dc.contributor.authorKANUNFRE, Kelly Aparecida
dc.contributor.authorRODRIGUES, Jonatas Cristian
dc.contributor.authorYAMAMOTO, Lidia
dc.contributor.authorNADAF, Maria Isabel Valdomir
dc.contributor.authorPALMEIRA, Patricia
dc.contributor.authorOKAY, Thelma Suely
dc.date.accessioned2019-01-17T13:35:19Z
dc.date.available2019-01-17T13:35:19Z
dc.date.issued2018
dc.description.abstractBacterial sepsis remains a major cause of mortality and blood cultures are the gold standard of laboratory diagnosis even though they lack sensitivity in neonates. Culture-negative sepsis, also known as clinical sepsis, has long been considered a diagnosis in neonatal intensive care units because, as well as culture-positive infants, culture-negative neonates have worse prognosis in comparison with non-infected ones. Quantitative amplifications are used to detect bacterial infections in neonates but results are considered only in a qualitative way (positive or negative). The aim of the present study was to determine and compare bacterial load levels in blood culture-positive and culture-negative neonatal sepsis. Seventy neonates with clinical and laboratory evidence of infection admitted at three neonatal intensive care units were classified as blood culture-positive or culture-negative. Blood samples obtained at the same time of blood cultures had bacterial load levels assessed through a 16S rDNA qPCR. Blood cultures were positive in 29 cases (41.4%) and qPCR in 64 (91.4%). In the 29 culture-positive cases, 100% were also positive by qPCR, while in the 41 culture-negative cases, 35 (85.4%) were positive by qPCR. Bacterial load levels were in general < 50 CFU/mL, but were significantly higher in culture-positive cases (Mann-Whitney, p = 0.013). although clinical and laboratory findings were similar, excepting for deaths. In conclusion, the present study has shown that blood culture-negative neonates have lower bacteria load levels in their bloodstream when compared to blood culture-positive infants.eng
dc.description.indexMEDLINEeng
dc.description.sponsorshipFAPEMAT (Mato Grosso State Research Foundation, Brazil) [009/2011, 752380/2011]
dc.description.sponsorshipAUX-PE-DINTER/NF from CAPES - Ministry of Education, Brazil [2535/2009]
dc.identifier.citationREVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, v.60, article ID UNSP e61, 10p, 2018
dc.identifier.doi10.1590/S1678-9946201860061
dc.identifier.eissn1678-9946
dc.identifier.issn0036-4665
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/30012
dc.language.isoeng
dc.publisherINST MEDICINA TROPICAL SAO PAULOeng
dc.relation.ispartofRevista do Instituto de Medicina Tropical de Sao Paulo
dc.rightsopenAccesseng
dc.rights.holderCopyright INST MEDICINA TROPICAL SAO PAULOeng
dc.subjectBlood cultureeng
dc.subjectCulture-negative neonatal sepsiseng
dc.subjectNeonatal blood stream infectioneng
dc.subjectReal Time PCReng
dc.subject16S rDNAeng
dc.subject.otherpolymerase-chain-reactioneng
dc.subject.otherreal-time pcreng
dc.subject.otherstream infectionseng
dc.subject.othermolecular assayseng
dc.subject.otherdiagnosiseng
dc.subject.otherchildreneng
dc.subject.otherinfantseng
dc.subject.otherbirtheng
dc.subject.othergeneeng
dc.subject.othervolumeeng
dc.subject.wosTropical Medicineeng
dc.titleAssessment and comparison of bacterial load levels determined by quantitative amplifications in blood culture-positive and negative neonatal sepsiseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.author.externalSTRANIERI, Ines:Univ Fed Mato Grosso, Hosp Univ Julio Muller, Lab Anal Clin, Div Microbiol, Cuiaba, Mato Grosso, Brazil
hcfmusp.author.externalRODRIGUES, Jonatas Cristian:Univ Sao Paulo, Inst Med Trop Sao Paulo, Lab Soroepidemiol & Imunobiol, Ave Doutor Eneas Carvalho Aguiar 470,Prodio 2, BR-05403000 Sao Paulo, SP, Brazil; Univ Sao Paulo, Fac Med, Dept Molestias Infecciosas & Parasitarias, LIM 48, Sao Paulo, SP, Brazil
hcfmusp.author.externalNADAF, Maria Isabel Valdomir:Univ Fed Mato Grosso, Fac Med, Dept Pediat, Cuiaba, Mato Grosso, Brazil
hcfmusp.citation.scopus21
hcfmusp.contributor.author-fmusphcKELLY APARECIDA KANUNFRE
hcfmusp.contributor.author-fmusphcLIDIA YAMAMOTO
hcfmusp.contributor.author-fmusphcPATRICIA PALMEIRA DAENEKAS JORGE
hcfmusp.contributor.author-fmusphcTHELMA SUELY OKAY
hcfmusp.description.articlenumberUNSP e61
hcfmusp.description.volume60
hcfmusp.origemWOS
hcfmusp.origem.pubmed30379228
hcfmusp.origem.scieloSCIELO:S0036-46652018005000235
hcfmusp.origem.scopus2-s2.0-85055657018
hcfmusp.origem.wosWOS:000448858700001
hcfmusp.publisher.citySAO PAULOeng
hcfmusp.publisher.countryBRAZILeng
hcfmusp.relation.referenceBard JD, 2016, J CLIN MICROBIOL, V54, P1418, DOI 10.1128/JCM.02919-15eng
hcfmusp.relation.referenceBurd EM, 2010, CLIN MICROBIOL REV, V23, P550, DOI 10.1128/CMR.00074-09eng
hcfmusp.relation.referenceButtery JP, 2002, ARCH DIS CHILD, V87, pF25, DOI 10.1136/fn.87.1.F25eng
hcfmusp.relation.referenceCantey JB, 2017, PEDIATRICS, V140, DOI 10.1542/peds.2017-0044eng
hcfmusp.relation.referenceCarey AJ, 2008, CLIN PERINATOL, V35, P223, DOI 10.1016/j.clp.2007.11.014eng
hcfmusp.relation.referenceChan KYY, 2009, CRIT CARE MED, V37, P2441, DOI 10.1097/CCM.0b013e3181a554deeng
hcfmusp.relation.referenceChen LH, 2009, CLIN PEDIATR, V48, P641, DOI 10.1177/0009922809333972eng
hcfmusp.relation.referenceConnell TG, 2007, PEDIATRICS, V119, P891, DOI 10.1542/peds.2006-0440eng
hcfmusp.relation.referenceCouto RC, 2007, AM J INFECT CONTROL, V35, P183, DOI 10.1016/j.ajic.2006.06.013eng
hcfmusp.relation.referenceGerdes JS, 2004, PEDIATR CLIN N AM, V51, P939, DOI 10.1016/j.pcl.2004.03.009eng
hcfmusp.relation.referenceGERDES JS, 1991, CLIN PERINATOL, V18, P361eng
hcfmusp.relation.referenceGoldstein B, 2006, PEDIATR CRIT CARE ME, V7, P200, DOI 10.1097/01.PCC.0000217470.68764.36eng
hcfmusp.relation.referenceHan YW, 2009, J CLIN MICROBIOL, V47, P38, DOI 10.1128/JCM.01206-08eng
hcfmusp.relation.referenceHofer N, 2012, NEONATOLOGY, V102, P25, DOI 10.1159/000336629eng
hcfmusp.relation.referenceJardine LA, 2009, J PAEDIATR CHILD H, V45, P210, DOI 10.1111/j.1440-1754.2008.01455.xeng
hcfmusp.relation.referenceJordan JA, 2005, J MOL DIAGN, V7, P575, DOI 10.1016/S1525-1578(10)60590-9eng
hcfmusp.relation.referenceKasper DC, 2013, NEONATOLOGY, V103, P268, DOI 10.1159/000346365eng
hcfmusp.relation.referenceKellogg JA, 1997, PEDIATR INFECT DIS J, V16, P381, DOI 10.1097/00006454-199704000-00009eng
hcfmusp.relation.referenceLeuthner SR, 2004, PEDIATR CLIN N AM, V51, P737, DOI 10.1016/j.pcl.2004.01.016eng
hcfmusp.relation.referenceLiu CL, 2014, ARCH PEDIATRIE, V21, P162, DOI 10.1016/j.arcped.2013.11.015eng
hcfmusp.relation.referenceLiu L, 2012, LANCET, V379, P2151, DOI 10.1016/S0140-6736(12)60560-1eng
hcfmusp.relation.referenceMANROE BL, 1979, J PEDIATR-US, V95, P89, DOI 10.1016/S0022-3476(79)80096-7eng
hcfmusp.relation.referenceMularoni A, 2014, PEDIATR INFECT DIS J, V33, pE121, DOI 10.1097/INF.0000000000000175eng
hcfmusp.relation.referenceMurray P, 1999, MANUAL CLIN MICROBIOeng
hcfmusp.relation.referenceNogueira CAM, 2004, REV PANAM INFECTOL, V6, P35eng
hcfmusp.relation.referenceOhlin A, 2008, ACTA PAEDIATR, V97, P1376, DOI 10.1111/j.1651-2227.2008.00924.xeng
hcfmusp.relation.referenceOhlin A, 2012, NEONATOLOGY, V101, P241, DOI 10.1159/000334655eng
hcfmusp.relation.referenceOzkan H, 2014, PEDIATR INT, V56, P60, DOI 10.1111/ped.12218eng
hcfmusp.relation.referencePammi M, 2011, PEDIATRICS, V128, pE973, DOI 10.1542/peds.2011-1208eng
hcfmusp.relation.referencePiantino JH, 2013, NEOREVIEWS, V14, pe294, DOI 10.1542/NE0.14-6-E294eng
hcfmusp.relation.referenceSchelonka RL, 1996, J PEDIATR-US, V129, P275, DOI 10.1016/S0022-3476(96)70254-8eng
hcfmusp.relation.referenceStranieri I, 2016, J MATERN-FETAL NEO M, V29, P2141, DOI 10.3109/14767058.2015.1077223eng
hcfmusp.relation.referenceTam PYI, 2017, PEDIATR RES, V82, P574, DOI 10.1038/pr.2017.134eng
hcfmusp.relation.referenceUnited Nations Children's Fund, 2008, STAT WORLDS CHILDR 2eng
hcfmusp.relation.referenceWorld Health Organization, 2010, WORLD HLTH STAT 2010eng
hcfmusp.relation.referenceWu YD, 2008, J CLIN MICROBIOL, V46, P2613, DOI 10.1128/JCM.02237-07eng
hcfmusp.relation.referenceZucol F, 2006, J CLIN MICROBIOL, V44, P2750, DOI 10.1128/JCM.00112-06eng
hcfmusp.scopus.lastupdate2024-04-12
relation.isAuthorOfPublication62e60a57-e9d1-4354-b9a9-bbdd6da549d0
relation.isAuthorOfPublicationa38bf500-fc7d-4398-ba8a-3c207001ac97
relation.isAuthorOfPublicationc4e95a40-a178-4b71-90f2-b9714cfe4ac5
relation.isAuthorOfPublication6cda0b34-04dd-4679-8fb3-faa904acb401
relation.isAuthorOfPublication.latestForDiscovery62e60a57-e9d1-4354-b9a9-bbdd6da549d0
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
art_STRANIERI_Assessment_and_comparison_of_bacterial_load_levels_determined_2018.PDF
Tamanho:
761.88 KB
Formato:
Adobe Portable Document Format
Descrição:
publishedVersion (English)
Baixar
Coleções
Artigos e Materiais de Revistas Científicas - FM/MPE
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/36
Artigos e Materiais de Revistas Científicas - LIM/48