BK virus salivary shedding and viremia in renal transplant recipients

Objectives: This study aimed to verify the presence of polyomavirus BK (BKPyV) in the saliva of kidney transplant recipients and to correlate it with blood viremia. Material and Methods: We have conducted a cross-sectional study with a sample involving 126 renal transplant recipients. 126 samples of saliva and 52 samples of blood were collected from these patients. Detection and quantification of BKPyV were performed using a real-time PCR. To compare the presence of BKPyV in blood and saliva, the binomial proportion test was used. To verify associations between salivary shedding BKPyV and post-transplant periods (in months), the Mann-Whitney test was used. Spearman's correlation was used to correlate the viral load in the saliva with blood of kidney transplant recipients. Results: The mean age of the study group was 51.11 +/- 12.45 years old, and 69 participants (54.8%) were female, with a mean post-transplantation time of 4.80 +/- 6.04 months. BKPyV was quantified in several samples of saliva and blood, with medians of 1,108 cp/mL and 1,255 cp/mL, respectively. Only 16/52 (30.8%) participants presented BKPyV in blood, and 59/126 (46.8%) excreted the virus in saliva (p=0.004). BKPyV shedding was found in patients at a shorter post-transplantation period (3.86 +/- 5.25, p=0.100). A weak correlation was observed between viral quantification in saliva and blood (Spearman's correlation coefficient=0.193). Conclusion: The results of this study suggested that, although saliva excretes more BKPyV than blood, there is no reliable correlation between salivary shedding and blood viremia, showing two independent compartments of viral replication.

Palavras-chave

Transplantation, BK virus, Saliva, Immunocompromised host, Polymerase chain reaction

URI

https://observatorio.fm.usp.br/handle/OPI/30856

Referências

Anastasopoulos NA, 2015, IN VIVO, V29, P415
Bassil N, 2014, EXP CLIN TRANSPLANT, V12, P212, DOI 10.6002/ect.2013.0252
Boukoum H, 2016, MICROB PATHOGENESIS, V97, P204, DOI 10.1016/j.micpath.2016.06.015
Castroa T, 2017, J CLIN VIROL, V89, P5, DOI 10.1016/j.jcv.2017.02.003
Chojnowska S, 2018, ADV MED SCI-POLAND, V63, P185, DOI 10.1016/j.advms.2017.11.002
Dugan AS, 2006, TRANSPL INFECT DIS, V8, P62, DOI 10.1111/j.1399-3062.2006.00153.x
Figueiredo M, 2017, ORAL DIS, V23, P1127, DOI 10.1111/odi.12707
Funahashi Y, 2014, TRANSPL P, V46, P564, DOI 10.1016/j.transproceed.2013.09.053
Gonzalez S, 2015, TRANSPL P, V47, P1777, DOI 10.1016/j.transproceed.2015.05.010
Hirsch HH, 2002, AM J TRANSPLANT, V2, P25, DOI 10.1034/j.1600-6143.2002.020106.x
Hsieh MC, 2013, MOL MED REP, V7, P1319, DOI 10.3892/mmr.2013.1334
Hu JH, 2011, EXP CLIN TRANSPLANT, V9, P175
Jeffers LK, 2009, VIROLOGY, V394, P183, DOI 10.1016/j.virol.2009.07.022
Jiang MX, 2009, VIROLOGY, V384, P266, DOI 10.1016/j.virol.2008.09.027
Martelli F, 2018, VIRUS RES, V249, P1, DOI 10.1016/j.virusres.2018.03.002
Nickeleit V, 2000, NEW ENGL J MED, V342, P1309, DOI 10.1056/NEJM200005043421802
Pal A, 2006, J VIROL METHODS, V135, P32, DOI 10.1016/j.jviromet.2006.01.018
Pires EP, 2011, TRANSPL INFECT DIS, V13, P633, DOI 10.1111/j.1399-3062.2011.00614.x
Robaina TF, 2013, EMERG INFECT DIS, V19, P155, DOI 10.3201/eid1901.120563
Saundh BK, 2010, J CLIN PATHOL, V63, P714, DOI 10.1136/jcp.2009.074864
Scadden Jacob Rw, 2017, World J Transplant, V7, P329, DOI 10.5500/wjt.v7.i6.329
Nunes LAS, 2015, BIOCHEM MEDICA, V25, P177, DOI 10.11613/BM.2015.018
Yalci A, 2015, TRANSPL P, V47, P1429, DOI 10.1016/j.transproceed.2015.04.056

Coleções

Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - HC/ICESP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/47
Artigos e Materiais de Revistas Científicas - LIM/52

Página do item completo