Sakuranetin reverses vascular peribronchial and lung parenchyma remodeling in a murine model of chronic allergic pulmonary inflammation

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorSAKODA, Camila Pivari Pedroso
dc.contributor.authorTOLEDO, Alessandra Choqueta de
dc.contributor.authorPERINI, Adenir
dc.contributor.authorPINHEIRO, Nathalia Montouro
dc.contributor.authorHIYANE, Meire Ioshie
dc.contributor.authorGRECCO, Simone dos Santos
dc.contributor.authorTIBERIO, Iolanda de Fatima Lopes Calvo
dc.contributor.authorCAMARA, Niels Olsen Saraiva
dc.contributor.authorMARTINS, Milton de Arruda
dc.contributor.authorLAGO, Joao Henrique Ghilardi
dc.contributor.authorRIGHETTI, Renato Fraga
dc.contributor.authorPRADO, Carla Maximo
dc.date.accessioned2016-12-20T16:36:07Z
dc.date.available2016-12-20T16:36:07Z
dc.date.issued2016
dc.description.abstractBackground and purpose: Asthma is a disease of high prevalence and morbidity that generates high costs in hospitalization and treatment. Although the airway is involved in the physiopathology of asthma, there is also evidence of the importance of vascular and lung parenchyma inflammation and remodeling, which can contribute to the functional pulmonary alterations observed in asthmatic patients. Our aim was to evaluate treatment using sakuranetin, a flavone isolated from the twigs of Baccharis retusa (Asteraceae), on vascular and lung parenchyma alterations in an experimental murine model of asthma. Methods: Male BALB/c mice were subjected to a sensitization protocol with ovalbumin for 30 days and were treated with or without sakuranetin (20 mg/kg/mice) or dexamethasone (5 mg/kg/mice); then, the lungs were collected for histopathological analysis. We evaluated extracellular matrix remodeling (collagen and elastic fibers), inflammation (eosinophils and NF-kB) and oxidative stress (8-isoprostane) in the pulmonary vessels and lung parenchyma. The thickness of the vascular wall was quantified, as well as the vascular endothelial growth factor (VEGF) levels. Results: We demonstrated that sakuranetin reduced the number of eosinophils and elastic fibers in both the pulmonary vessels and the lung parenchyma, probably due to a reduction of oxidative stress and of the transcription factor NF-kB and VEGF levels in the lung. In addition, it reduced the thickness of the pulmonary vascular wall. The treatment had no effect on the collagen fibers. In most of the parameters, the effect of sakuranetin was similar to the dexamethasone effect. Conclusions and implications: Sakuranetin had anti-inflammatory and antioxidant effects, preventing vascular and distal parenchyma changes in this experimental model of asthma.
dc.description.indexMEDLINE
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2010/14831-3, 2008/55359-5, 2014/25689-4]
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [304465/2012-7, 476877/2012-1]
dc.identifier.citationACTA HISTOCHEMICA, v.118, n.6, p.615-624, 2016
dc.identifier.doi10.1016/j.acthis.2016.07.001
dc.identifier.eissn1618-0372
dc.identifier.issn0065-1281
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/17012
dc.language.isoeng
dc.publisherELSEVIER GMBH, URBAN & FISCHER VERLAG
dc.relation.ispartofActa Histochemica
dc.rightsrestrictedAccess
dc.rights.holderCopyright ELSEVIER GMBH, URBAN & FISCHER VERLAG
dc.subjectBronchial asthma
dc.subjectSakuranetin
dc.subjectRemodeling
dc.subjectOxidative stress
dc.subjectPeribronchial vessels
dc.subjectLung parenchyma
dc.subject.othernf-kappa-b
dc.subject.otherairway inflammation
dc.subject.otheroxidative stress
dc.subject.othermast-cells
dc.subject.otherasthma
dc.subject.otherflavonoids
dc.subject.otherinhibition
dc.subject.otherasteraceae
dc.subject.otherluteolin
dc.subject.otherdisease
dc.subject.wosCell Biology
dc.titleSakuranetin reverses vascular peribronchial and lung parenchyma remodeling in a murine model of chronic allergic pulmonary inflammation
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalSAKODA, Camila Pivari Pedroso:Univ Fed Sao Paulo, Dept Biosci, Santos, SP, Brazil
hcfmusp.author.externalHIYANE, Meire Ioshie:Univ Sao Paulo, Inst Biol, Dept Immunol, Sao Paulo, SP, Brazil
hcfmusp.author.externalGRECCO, Simone dos Santos:Univ Fed Sao Paulo, Dept Biosci Exact & Earth Sci, Diadema, Brazil
hcfmusp.author.externalCAMARA, Niels Olsen Saraiva:Univ Sao Paulo, Inst Biol, Dept Immunol, Sao Paulo, SP, Brazil
hcfmusp.author.externalLAGO, Joao Henrique Ghilardi:Univ Fed Sao Paulo, Dept Biosci Exact & Earth Sci, Diadema, Brazil
hcfmusp.author.externalPRADO, Carla Maximo:Univ Fed Sao Paulo, Dept Biosci, Santos, SP, Brazil
hcfmusp.citation.scopus24
hcfmusp.contributor.author-fmusphcALESSANDRA CHOQUETA DE TOLEDO ARRUDA
hcfmusp.contributor.author-fmusphcADENIR PERINI
hcfmusp.contributor.author-fmusphcNATHALIA MONTOURO PINHEIRO MENEGASSO
hcfmusp.contributor.author-fmusphcIOLANDA DE FATIMA LOPES CALVO TIBERIO
hcfmusp.contributor.author-fmusphcMILTON DE ARRUDA MARTINS
hcfmusp.contributor.author-fmusphcRENATO FRAGA RIGHETTI
hcfmusp.description.beginpage615
hcfmusp.description.endpage624
hcfmusp.description.issue6
hcfmusp.description.volume118
hcfmusp.origemWOS
hcfmusp.origem.pubmed27425653
hcfmusp.origem.scopus2-s2.0-84978808470
hcfmusp.origem.wosWOS:000383010800008
hcfmusp.publisher.cityJENA
hcfmusp.publisher.countryGERMANY
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