Molecular and immunohistochemical analysis of the urethra of female rats after induced trauma and intravenous therapy with muscle derived stem cells

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorBILHAR, Andreisa P. M.
dc.contributor.authorBORTOLINI, Maria A. T.
dc.contributor.authorSE, Alexandre B.
dc.contributor.authorFEITOSA, Suellen M.
dc.contributor.authorSALERNO, Gisela R. F.
dc.contributor.authorZANOTELI, Edmar
dc.contributor.authorSIMOES, Manuel J.
dc.contributor.authorCASTRO, Rodrigo A.
dc.date.accessioned2018-11-21T17:04:44Z
dc.date.available2018-11-21T17:04:44Z
dc.date.issued2018
dc.description.abstractAimsTo identify the urethral migration of muscle derived stem cells (MDSCs) after intravenous (IV) injection in rats that underwent vaginal distension (VD) and to analyze the effects of MDSC in the urethra of rats after trauma in regards to: (1) mRNA expression of collagens, Vegf, Ngf, Ki67, Myh11, and Myh2; (2) expression of smooth and striated muscle proteins. MethodsMDSCs expressing green fluorescent protein (GFP) were injected into the tail vein of rats 3 days after VD. The location of GFP cells was verified at 2h and at 7 days following IV injection. Urethras of three groups were analyzed: Control, Trauma 7D, and MDSC 7D. Real-time RT-qPCR and immunohistochemistry were performed. ResultsMDSCs were identified only after 2h of the procedure in the urethra. Myh11 gene was overexpressed in the Trauma group in relation to Control. Ki67 gene expression was increased in the MDSC group relative to Trauma and Control. Col1a1 and Col3a1 genes expression were increased in the MDSC group relative to Control. Ngf mRNA level was decreased in the MDSC group in relation to Trauma. Protein expression of Mhy11, Myh2, and Desmin were increased in the MDSC group in relation to Trauma and decreased in the Trauma in relation to Control. ConclusionMDSCs migrated early to the traumatized urethra, but did not integrate into the tissue. MDSC alters the expression of genes related to cell proliferation, neural growth factor and extracellular matrix and the expression of smooth and striated muscle proteins in the traumatized rat urethra.
dc.description.indexMEDLINE
dc.identifier.citationNEUROUROLOGY AND URODYNAMICS, v.37, n.7, p.2151-2159, 2018
dc.identifier.doi10.1002/nau.23567
dc.identifier.eissn1520-6777
dc.identifier.issn0733-2467
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/29495
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofNeurourology and Urodynamics
dc.rightsrestrictedAccess
dc.rights.holderCopyright WILEY
dc.subjectadult stem cell
dc.subjectregenerative medicine
dc.subjecturethra
dc.subjecturinary incontinence
dc.subject.otherstress urinary-incontinence
dc.subject.otherintrinsic sphincter deficiency
dc.subject.othersimulated childbirth injury
dc.subject.otherleak point pressure
dc.subject.othernerve growth-factor
dc.subject.othervaginal distension
dc.subject.othermodel
dc.subject.otherinjection
dc.subject.othercontractility
dc.subject.otherexpression
dc.subject.wosUrology & Nephrology
dc.titleMolecular and immunohistochemical analysis of the urethra of female rats after induced trauma and intravenous therapy with muscle derived stem cells
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalBILHAR, Andreisa P. M.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil
hcfmusp.author.externalBORTOLINI, Maria A. T.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil
hcfmusp.author.externalSE, Alexandre B.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil
hcfmusp.author.externalFEITOSA, Suellen M.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil
hcfmusp.author.externalSALERNO, Gisela R. F.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil
hcfmusp.author.externalSIMOES, Manuel J.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil
hcfmusp.author.externalCASTRO, Rodrigo A.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil
hcfmusp.citation.scopus5
hcfmusp.contributor.author-fmusphcEDMAR ZANOTELI
hcfmusp.description.beginpage2151
hcfmusp.description.endpage2159
hcfmusp.description.issue7
hcfmusp.description.volume37
hcfmusp.origemWOS
hcfmusp.origem.pubmed30152522
hcfmusp.origem.scopus2-s2.0-85053211076
hcfmusp.origem.wosWOS:000444957900012
hcfmusp.publisher.cityHOBOKEN
hcfmusp.publisher.countryUSA
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