Polysaccharide diversity in VNI isolates of Cryptococcus neoformans from Roraima, Northern Brazil

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art_PUPO_Polysaccharide_diversity_in_VNI_isolates_of_Cryptococcus_neoformans_2019.PDF (2.53 MB)
Citações na Scopus
6
Tipo de produção
article
Data de publicação
2019
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER SCI LTD
Autores
PUPO, Halan D. Dal
SENA, Bianca A. G.
REIS, Flavia C. G.
FORTES, Silvana T.
GODINHO, Rodrigo M. C.
RODRIGUES, Marcio L.
Citação
FUNGAL BIOLOGY, v.123, n.10, p.699-708, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Species of the Cryptococcus genus comprise environmental, encapsulated fungal pathogens that cause lethal meningitis in immunosuppressed individuals. In humans, fungal uptake of hypocapsular Cryptococcus by macrophages was associated with high fungal burden in the cerebrospinal fluid and long-term patient survival. On the basis of the key role of the cryptococcal capsule in disease, we analyzed the diversity of capsular structures in 23 isolates from pigeon excreta collected in the cities of Boa Vista, Bonfim and Pacaraima, in the state of Roraima (Northern Brazil). All isolates were identified as Cryptococcus neoformans (VNI genotype) by MALDI-TOF mass spectrometry. Through a combination of fluorescence microscopy, flow cytometry, ELISA and spectrophotometric methods, each isolate was characterized at the phenotypical level, which included measurements of growth rates at 30 and 37 degrees C, pigmentation, cell body size, capsular dimensions, serological reactivity, urease production and ability to produce extracellular glucuronoxylomannan (GXM), the main capsular component of C. neoformans. With the exception of melanization, a formidable diversity was observed considering all parameters tested in our study. Of note, hyper and hypo producers of GXM were identified, in addition to isolates with hyper and hypo profiles of reactivity with a polysaccharide-binding monoclonal antibody. Capsular dimensions were also highly variable in the collection of isolates. Extracellular GXM production correlated positively with capsular dimensions, urease activity and cell size. Unexpectedly, GXM concentrations did not correlate with serological reactivity with the cryptococcal capsule. These results reveal a high diversity in the ability of environmental C. neoforrnans to produce capsular components, which might impact the outcome of human cryptococcosis.
Palavras-chave
Capsule, Cryptococcal diversity, Glucuronoxylomannan, Glycan heterogeneity
URI
https://observatorio.fm.usp.br/handle/OPI/34146
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Coleções
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/03
Artigos e Materiais de Revistas Científicas - LIM/53