Evaluation of larval surface antigens from infective larvae of Strongyloides venezuelensis for the serodiagnosis of human strongyloidiasis

Serodiagnosis of strongyloidiasis is usually performed by ELISA for the detection of IgG antibodies due to its high sensitivity and practicality, but its main limitation is a constant source of S. stercoralis antigens. The use of S. venezuelensis as a heterologous source of antigens has facilitated several published studies on the serodiagnosis and epidemiology of human strongyloidiasis. The main objective of this study was to evaluate the diagnostic accuracy of surface cuticle antigens of infective larvae of S. venezuelensis extracted with CTAB detergent (L3-CTAB) in comparison with soluble somatic extracts (L3-SSE) using a panel of sera from immunocompetent and immunocompromised individuals, at three different cut-offs. ROC curve analysis showed that L3-CTAB had an AUC of 0.9926. At the first cut-off value (OD 450 nm = 0.214), sensitivity and specificity were 100% and 90.11%, respectively, with a diagnostic accuracy of 0.93. At a second cut-off value (OD 450 nm = 0.286), sensitivity and specificity were 70% and 100%, respectively, with a diagnostic accuracy of 0.91. However, at an alternative third cut-off value (OD 450 nm = 0.589), sensitivity and specificity were 95% and 97.8%, respectively, with a diagnostic accuracy of 0.97. Using L3-CTAB as an antigenic source, the seropositivity rate in immunocompromised patients was 28.13% (9/32) whereas a seropositivity rate of 34.38% (11/32) was found when L3-SSE was used in ELISA. Therefore, the L3-CTAB is simple and practical to obtain and was found to be highly sensitive and specific.

Palavras-chave

Strongyloidiasis, Strongyloides venezuelensis, Surface, Enzyme-linked, immunosorbent assay, Serodiagnosis

URI

https://observatorio.fm.usp.br/handle/OPI/52964

Referências

Buonfrate D, 2020, PATHOGENS, V9, DOI 10.3390/pathogens9060468
Casado L, 2019, INT J INFECT DIS, V88, P60, DOI 10.1016/j.ijid.2019.09.003
Corral MA, 2017, PARASITOLOGY, V144, P124, DOI 10.1017/S0031182016001645
Corral MA, 2015, REV INST MED TROP SP, V57, P77, DOI 10.1590/S0036-46652015000100011
Cui J, 2013, PARASITE VECTOR, V6, DOI 10.1186/1756-3305-6-355
Eamudomkarn C, 2015, PARASITOL RES, V114, P2543, DOI 10.1007/s00436-015-4458-3
Eusebi P, 2013, CEREBROVASC DIS, V36, P267, DOI 10.1159/000353863
Feliciano ND, 2010, DIAGN MICR INFEC DIS, V67, P153, DOI 10.1016/j.diagmicrobio.2010.01.012
Garcia LS, 2001, DIAGNOSTIC MED PARAS
GRENCIS RK, 1986, PARASITE IMMUNOL, V8, P587, DOI 10.1111/j.1365-3024.1986.tb00872.x
Guillamet LJV, 2017, AM J CASE REP, V18, P339, DOI 10.12659/AJCR.902626
Kalantari N, 2020, T ROY SOC TROP MED H, V114, P459, DOI 10.1093/trstmh/trz135
KOGA K, 1991, AM J TROP MED HYG, V45, P518, DOI 10.4269/ajtmh.1991.45.518
Liu RD, 2015, ACTA TROP, V150, P79, DOI 10.1016/j.actatropica.2015.07.002
Luvira V, 2016, AM J TROP MED HYG, V95, P401, DOI 10.4269/ajtmh.16-0068
Machado ER, 2003, MEM I OSWALDO CRUZ, V98, P849, DOI 10.1590/S0074-02762003000600024
Marcos Luis A, 2011, Curr Infect Dis Rep, V13, P35, DOI 10.1007/s11908-010-0150-z
MURRELL KD, 1983, EXP PARASITOL, V55, P331, DOI 10.1016/0014-4894(83)90030-9
NORTHERN C, 1989, CLIN EXP IMMUNOL, V75, P487
Nunes JB, 2018, REV SOC BRAS MED TRO, V51, P855, DOI 10.1590/0037-8682-0090-2018
PRITCHARD DI, 1985, PARASITE IMMUNOL, V7, P575, DOI 10.1111/j.1365-3024.1985.tb00101.x
Ramanathan Roshan, 2008, Curr Infect Dis Rep, V10, P105, DOI 10.1007/s11908-008-0019-6
Requena-Mendez A, 2013, PLOS NEGLECT TROP D, V7, DOI 10.1371/journal.pntd.0002002
Gonzales WHR, 2021, PARASITOLOGY, V148, P1522, DOI 10.1017/S0031182021001207
Vasquez-Rios George, 2019, J Parasit Dis, V43, P167, DOI 10.1007/s12639-019-01090-x

Coleções

Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - LIM/06

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