Bothrops jararaca Venom Metalloproteinases Are Essential for Coagulopathy and Increase Plasma Tissue Factor Levels during Envenomation

Imagem de Miniatura
Arquivos
art_YAMASHITA_Bothrops_jararaca_Venom_Metalloproteinases_Are_Essential_for_Coagulopathy_2014.PDF (583.04 KB)
Citações na Scopus
50
Tipo de produção
article
Data de publicação
2014
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
PUBLIC LIBRARY SCIENCE
Autores
ALVES, Andre F.
BARBARO, Katia C.
Citação
PLOS NEGLECTED TROPICAL DISEASES, v.8, n.5, article ID e2814, 12p, 2014
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background/Aims: Bleeding tendency, coagulopathy and platelet disorders are recurrent manifestations in snakebites occurring worldwide. We reasoned that by damaging tissues and/or activating cells at the site of the bite and systemically, snake venom toxins might release or decrypt tissue factor (TF), resulting in activation of blood coagulation and aggravation of the bleeding tendency. Thus, we addressed (a) whether TF and protein disulfide isomerase (PDI), an oxireductase involved in TF encryption/decryption, were altered in experimental snake envenomation; (b) the involvement and significance of snake venom metalloproteinases (SVMP) and serine proteinases (SVSP) to hemostatic disturbances. Methods/Principal Findings: Crude Bothrops jararaca venom (BjV) was preincubated with Na-2-EDTA or AEBSF, which are inhibitors of SVMP and SVSP, respectively, and injected subcutaneously or intravenously into rats to analyze the contribution of local lesion to the development of hemostatic disturbances. Samples of blood, lung and skin were collected and analyzed at 3 and 6 h. Platelet counts were markedly diminished in rats, and neither Na-2-EDTA nor AEBSF could effectively abrogate this fall. However, Na-2-EDTA markedly reduced plasma fibrinogen consumption and hemorrhage at the site of BjV inoculation. Na-2-EDTA also abolished the marked elevation in TF levels in plasma at 3 and 6 h, by both administration routes. Moreover, increased TF activity was also noticed in lung and skin tissue samples at 6 h. However, factor VII levels did not decrease over time. PDI expression in skin was normal at 3 h, and downregulated at 6 h in all groups treated with BjV. Conclusions: SVMP induce coagulopathy, hemorrhage and increased TF levels in plasma, but neither SVMP nor SVSP are directly involved in thrombocytopenia. High levels of TF in plasma and TF decryption occur during snake envenomation, like true disseminated intravascular coagulation syndrome, and might be implicated in engendering bleeding manifestations in severely-envenomed patients.
Palavras-chave
URI
https://observatorio.fm.usp.br/handle/OPI/7716
Referências
  1. Aldridge GM, 2008, J NEUROSCI METH, V172, P250, DOI 10.1016/j.jneumeth.2008.05.003
  2. Anai K, 2002, TOXICON, V40, P63, DOI 10.1016/S0041-0101(01)00189-1
  3. Antunes TC, 2010, TOXICON, V56, P1443, DOI 10.1016/j.toxicon.2010.08.011
  4. BARRANTES A, 1985, TOXICON, V23, P399, DOI 10.1016/0041-0101(85)90024-8
  5. Berger M, 2008, TOXICON, V51, P488, DOI [10.1016/j.toxicon.2007.09.005, 10.1016/j.toxicon.2007-09.005]
  6. Birrell GW, 2007, MOL CELL PROTEOMICS, V6, P973, DOI 10.1074/mcp.M600419-MCP200
  7. BLOMBACK B, 1957, THROMB DIATH HAEMOST, V1, P76
  8. Breitenstein A, 2010, CLIN SCI, V118, P159, DOI 10.1042/CS20080622
  9. Cho J, 2012, BLOOD, V120, P647, DOI 10.1182/blood-2011-08-372532
  10. Cidade DAP, 2006, TOXICON, V48, P437, DOI 10.1016/j.toxicon.2006.07.008
  11. DEMPFLE CE, 1990, BLUT, V61, P369, DOI 10.1007/BF01738552
  12. Denson KWE, 1976, HUMAN BLOOD COAGULAT, P657
  13. Eagle H, 1937, J EXP MED, V65, P613, DOI 10.1084/jem.65.5.613
  14. Egorina EM, 2011, BLOOD COAGUL FIBRIN, V22, P451, DOI 10.1097/MBC.0b013e328346ef3f
  15. GOLD AM, 1964, BIOCHEMISTRY-US, V3, P783, DOI 10.1021/bi00894a009
  16. Gutierrez JM, 2005, TOXICON, V45, P997, DOI 10.1016/j.toxicon.2005.02.029
  17. Habermann E, 1958, ARCH EXP PATHOL PH, V234, P291
  18. HAWGOOD BJ, 1995, TOXICON, V33, P591, DOI 10.1016/0041-0101(95)00006-8
  19. HENRIQUES OB, 1960, BIOCHEM J, V75, P551
  20. HENRIQUES OB, 1959, NATURE, V183, P114, DOI 10.1038/183114a0
  21. Herrera C, 2013, TOXICON, V63, P19, DOI 10.1016/j.toxicon.2012.10.023
  22. HO M, 1986, TOXICON, V24, P211, DOI 10.1016/0041-0101(86)90147-9
  23. HOFMANN H, 1987, BIOCHEMISTRY-US, V26, P780, DOI 10.1021/bi00377a019
  24. Isbister GK, 2010, J THROMB HAEMOST, V8, P2504, DOI 10.1111/j.1538-7836.2010.04050.x
  25. Isbister GK, 2010, SEMIN THROMB HEMOST, V36, P444, DOI 10.1055/s-0030-1254053
  26. JANSZKY B, 1950, SCIENCE, V112, P173, DOI 10.1126/science.112.2902.173
  27. JERN C, 1989, THROMB HAEMOSTASIS, V62, P767
  28. Kamiguti AS, 1996, BIOCHEM J, V320, P635
  29. KAMIGUTI AS, 1992, THROMB HAEMOSTASIS, V67, P484
  30. KAMIGUTI AS, 1991, TOXICON, V29, P961, DOI 10.1016/0041-0101(91)90079-7
  31. KAMIGUTI AS, 1991, TOXICON, V29, P1097, DOI 10.1016/0041-0101(91)90207-8
  32. LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0
  33. Levi M, 1999, NEW ENGL J MED, V341, P586, DOI 10.1056/NEJM199908193410807
  34. Lira MS, 2007, TOXICON, V49, P982, DOI 10.1016/j.toxicon.2007.01.009
  35. Loria GD, 2003, ARCH BIOCHEM BIOPHYS, V418, P13, DOI 10.1016/S0003-9861(03)00385-0
  36. Luna MS, 2013, J PROTEOMICS, V94, P460, DOI 10.1016/j.jprot.2013.10.026
  37. Maruyama M, 2002, TOXICON, V40, P1223, DOI 10.1016/S0041-0101(02)00116-2
  38. MARUYAMA M, 1990, THROMB HAEMOSTASIS, V63, P449
  39. Mellanby J, 1909, J PHYSIOL-LONDON, V38, P441
  40. Menaldo DL, 2012, BIOCHIMIE, V94, P2545, DOI 10.1016/j.biochi.2012.07.007
  41. Miller JN, 2010, STAT CHEMOMETRICS AN, P110
  42. NAHAS L, 1975, TOXICON, V13, P457, DOI 10.1016/0041-0101(75)90175-0
  43. NAHAS L, 1979, THROMB HAEMOSTASIS, V41, P314
  44. Pereira ALM, 2006, TOXICON, V47, P788, DOI 10.1016/j.toxicon.2006.02.014
  45. Petricevich VL, 2000, TOXICON, V38, P1253, DOI 10.1016/S0041-0101(99)00227-5
  46. Popescu NI, 2010, BLOOD, V116, P993, DOI 10.1182/blood-2009-10-249607
  47. Ratnoff OD, 1951, J LAB CLIN MED, V37, P216
  48. REDINBAUGH MG, 1986, ANAL BIOCHEM, V153, P267, DOI 10.1016/0003-2697(86)90091-6
  49. Reinhardt C, 2008, J CLIN INVEST, V118, P1110, DOI 10.1172/JCI32376
  50. Resiere D, 2010, TOXINS, V2, P163, DOI 10.3390/toxins2010163
  51. RIBEIRO L A, 1989, Revista do Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, V44, P143
  52. Rosenfeld G, 1968, VENOMOUS ANIMALS THE, P229
  53. ROSENFELD G., 1959, MEM INST BUTANTAN, V29, P143
  54. Rucavado A, 2004, TOXICON, V43, P417, DOI 10.1016/j.toxicon.2004.01.016
  55. Rucavado A, 2005, THROMB HAEMOSTASIS, V94, P123
  56. Rucavado A, 2000, AM J TROP MED HYG, V63, P313
  57. Rucavado A, 2008, TOXICON, V52, P754, DOI 10.1016/j.toxicon.2008.08.009
  58. Sanchez EF, 2010, ARCH BIOCHEM BIOPHYS, V496, P9, DOI 10.1016/j.abb.2010.01.010
  59. SANDERS WE, 1988, LAB INVEST, V59, P443
  60. SanoMartins IS, 1997, THROMB RES, V87, P183, DOI 10.1016/S0049-3848(97)00118-7
  61. Santoro ML, 2004, J IMMUNOL METHODS, V284, P55, DOI 10.1016/j.jim.2003.10.005
  62. Santoro ML, 1999, COMP BIOCHEM PHYS C, V122, P61, DOI 10.1016/S0742-8413(98)10079-8
  63. Santoro ML, 2008, TOXICON, V51, P1440, DOI 10.1016/j.toxicon.2008.03.018
  64. Santoro ML, 2004, THROMB HAEMOSTASIS, V92, P369, DOI 10.1160/TH04-02-0120
  65. SANTORO ML, 1993, TOXICON, V31, P733, DOI 10.1016/0041-0101(93)90379-W
  66. Serrano SMT, 2005, TOXICON, V45, P1115, DOI 10.1016/j.toxicon.2005.02.020
  67. SERRANO SMT, 1995, BIOCHEMISTRY-US, V34, P7186, DOI 10.1021/bi00021a033
  68. SERRANO SMT, 1993, TOXICON, V31, P471, DOI 10.1016/0041-0101(93)90182-I
  69. Shimura M, 1997, AM J HEMATOL, V55, P169, DOI 10.1002/(SICI)1096-8652(199707)55:4<169::AID-AJH1>3.0.CO;2-Q
  70. SIMON TL, 1981, NEW ENGL J MED, V305, P443, DOI 10.1056/NEJM198108203050808
  71. Stocker K, 1978, HDB EXPT PHARM, V46, P451
  72. Stocker KF, 1988, HEMOSTASIS ANIMAL VE, P525
  73. Takeda S, 2012, BBA-PROTEINS PROTEOM, V1824, P164, DOI 10.1016/j.bbapap.2011.04.009
  74. Than-Than, 1988, BRIT J HAEMATOL, V69, P513
  75. von Klobusitzky D, 1936, N-S ARCH EX PATH PH, V181, P387
  76. WARRELL DA, 1977, Q J MED, V46, P33
  77. Yamashita KM, 2011, J THROMB HAEMOST, V9, P2057, DOI 10.1111/j.1538-7836.2011.04449.x
  78. Yingprasertchai S, 2003, TOXICON, V42, P635, DOI 10.1016/j.toxicon.2003.09.001
  79. Zelanis A, 2012, PLOS NEGLECT TROP D, V6, DOI 10.1371/journal.pntd.0001554
  80. Zhang B, 2006, BMC GENOMICS, V7, DOI 10.1186/1471-2164-7-152

Coleções
Artigos e Materiais de Revistas Científicas - FM/Outros