Histopathological characteristics of cutaneous lesions caused by Leishmania Viannia panamensis in Panama

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art_GONZÁLEZ_Histopathological_characteristics_of_cutaneous_lesions_caused_by_Leishmania_2018.PDF (4.6 MB)
Citações na Scopus
22
Tipo de produção
article
Data de publicação
2018
DOI
SciELO
Scopus
Título da Revista
ISSN da Revista
Título do Volume
Editora
Instituto de Medicina Tropical de São Paulo
Autores
DIAZ, Rosendo
GARCÍA, Víctor
PAZ, Héctor
CALZADA, José E.
RUÍZ, Michelle
SALDAñA, Azael
Citação
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SãO PAULO, v.60, p.e8, 2018
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
ABSTRACT Cutaneous leishmaniasis (CL) is an endemic disease in the Republic of Panama, caused by Leishmania (Viannia) parasites, whose most common clinical manifestation is the presence of ulcerated lesions on the skin. These lesions usually present a chronic inflammatory reaction, sometimes granulomatous, with the presence of lymphocytes, plasma cells and macrophages. This study describes the histopathological characteristics found in the skin lesions of patients with CL caused by Leishmania (V.) panamensis in Panama. We analyzed 49 skin biopsy samples from patients with clinical suspicion of CL, by molecular tests (PCR for subgenus Viannia and HSP-70) and by Hematoxylin-Eosin staining. Samples were characterized at the species level by PCR-HSP-70/RFLP. From the 49 samples studied, 46 (94%) were positive by PCR and were characterized as Leishmania (V.) panamensis. Of these, 48% were positive by Hematoxylin-Eosin staining with alterations being observed both, in the epidermis (85%) and in the dermis (100%) of skin biopsies. The inflammatory infiltrate was characterized according to histopathological patterns: lymphohistiocytic (50%), lymphoplasmacytic (61%) and granulomatous (46%) infiltration, being the combination of these patterns frequently found. The predominant histopathological characteristics observed in CL lesions caused by L. (V.) panamensis in Panama were: an intense inflammatory reaction in the dermis with a combination of lymphohistiocytic, lymphoplasmacytic and granulomatous presentation patterns and the presence of ulcers, acanthosis, exocytosis and spongiosis in the epidermis.
Palavras-chave
Cutaneous leishmaniasis, Leishmania Viannia panamensis, Histopathology, Panama
URI
https://observatorio.fm.usp.br/handle/OPI/55835
Referências
  1. Bates LB, 1913, J AMER MED ASSOC, V60, P898, DOI 10.1001/jama.1913.04340120024013
  2. Bern C, 2008, PLOS NEGLECT TROP D, V2, DOI 10.1371/journal.pntd.0000313
  3. BITTENCOURT AL, 1991, MEM I OSWALDO CRUZ, V86, P51, DOI 10.1590/S0074-02761991000100009
  4. Gomes CMC, 2017, PARASITOL RES, V116, P1423, DOI 10.1007/s00436-017-5403-4
  5. Darling ST, 1911, J AMER MED ASSOC, V56, P1257
  6. Darling ST, 1911, ARCH INTERN MED, V7, P581
  7. de Y., 2010, REV MED CIENTIFICA, V23, P45
  8. DEMAGALHAES AV, 1986, REV I MED TROP, V28, P421, DOI 10.1590/S0036-46651986000600008
  9. Engel KB, 2011, ARCH PATHOL LAB MED, V135, P537, DOI 10.1043/2010-0702-RAIR.1
  10. Esperanza Azogue C, 1983, Mem Inst Oswaldo Cruz, V78, P13, DOI 10.1590/S0074-02761983000100002
  11. Fox H, 1931, ARCH DERMATOL SYPH, V23, P480, DOI 10.1001/archderm.1931.03880210073007
  12. Garcia L, 2004, J CLIN MICROBIOL, V42, P2294, DOI 10.1128/JCM.42.5.2294-2297.2004
  13. Gonzalez Kadir, 2015, Tropical Medicine and Health, V43, P75, DOI 10.2149/tmh.2014-30
  14. Grevelink SA, 1996, J AM ACAD DERMATOL, V34, P257, DOI 10.1016/S0190-9622(96)80121-6
  15. GRIMALDI G, 1989, AM J TROP MED HYG, V41, P687, DOI 10.4269/ajtmh.1989.41.687
  16. Martins Ana Luiza Grizzo Peres, 2014, An. Bras. Dermatol., V89, P52, DOI 10.1590/abd1806-4841.20142226
  17. Handler MZ, 2015, J AM ACAD DERMATOL, V73, P911, DOI 10.1016/j.jaad.2014.09.014
  18. HERRICK AB, 1911, P M A ISTHMIAN CANAL, V3, P21
  19. Hofman W, 2003, AM J CLIN PATHOL, V120, P567, DOI 10.1309/R3DK4MR3W6E5PH17
  20. Isaza DM, 1996, AM J TROP MED HYG, V55, P365, DOI 10.4269/ajtmh.1996.55.365
  21. KURBAN AK, 1966, ARCH DERMATOL, V93, P396, DOI 10.1001/archderm.93.4.396
  22. Machado P, 2002, EUR J DERMATOL, V12, P449
  23. Mansour L, 1993, J Egypt Soc Parasitol, V23, P591
  24. Mathers CD, 2007, PLOS NEGLECT TROP D, V1, DOI 10.1371/journal.pntd.0000114
  25. Mehregan DR, 1999, CLIN DERMATOL, V17, P297, DOI 10.1016/S0738-081X(99)00048-6
  26. Miranda A, 2009, AM J TROP MED HYG, V81, P565, DOI 10.4269/ajtmh.2009.08-0265
  27. Montalvo AM, 2010, PARASITOLOGY, V137, P1159, DOI 10.1017/S0031182010000089
  28. Alvarez AMM, 2010, T ROY SOC TROP MED H, V104, P364, DOI 10.1016/j.trstmh.2009.12.002
  29. Morgado FN, 2008, BRIT J DERMATOL, V158, P50, DOI 10.1111/j.1365-2133.2007.08255.x
  30. Oliveira Milton Adriano Pelli de, 2010, Rev. Inst. Med. trop. S. Paulo, V52, P83, DOI 10.1590/S0036-46652010000200004
  31. Quintella Leonardo P., 2009, Rev. Inst. Med. trop. S. Paulo, V51, P83, DOI 10.1590/S0036-46652009000200005
  32. Ramirez JR, 2000, J CLIN MICROBIOL, V38, P3768
  33. RIDLEY DS, 1979, T ROY SOC TROP MED H, V73, P150, DOI 10.1016/0035-9203(79)90199-8
  34. Alves CRR, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0076301
  35. Saldanha MG, 2017, AM J TROP MED HYG, V96, P645, DOI 10.4269/ajtmh.16-0539
  36. Schubach A, 2001, MEM I OSWALDO CRUZ, V96, P987, DOI 10.1590/S0074-02762001000700018
  37. Silveira FT, 2009, PARASITE IMMUNOL, V31, P423, DOI 10.1111/j.1365-3024.2009.01116.x
  38. Silveira FT, 2008, T ROY SOC TROP MED H, V102, P1075, DOI 10.1016/j.trstmh.2008.05.020
  39. Silveira Fernando T, 2004, Mem. Inst. Oswaldo Cruz, V99, P239, DOI 10.1590/S0074-02762004000300001
  40. SNOW JS, 1948, ARCH DERMATOL SYPH, V57, P90, DOI 10.1001/archderm.1948.01520130093008
  41. SOTTO MN, 1989, ACTA TROP, V46, P121, DOI 10.1016/0001-706X(89)90006-5
  42. TAPIA FJ, 1994, IMMUNOL TODAY, V15, P160, DOI 10.1016/0167-5699(94)90312-3
  43. THORNBURGH DB, 1952, T ROY SOC TROP MED H, V46, P550, DOI 10.1016/0035-9203(52)90048-5
  44. Tresserra F., 2016, REV SENOL PATOL MAMA, V29, P26, DOI [10.1016/j.senol.2015.11.004, DOI 10.1016/J.SENOL.2015.11.004]
  45. Venkataram M, 2001, Indian J Dermatol Venereol Leprol, V67, P294
  46. Vergel C, 2005, AM J TROP MED HYG, V72, P423, DOI 10.4269/ajtmh.2005.72.423
  47. Xavier S.C., 2006, BMC VET RES, V8, P2, DOI 10.1186/1746-6148-2-17
  48. Yuil JMR., 2012, DERMATOLOGIA COSMET, V10, P13
  49. 2014, Informe final: segunda reunión de gerentes de programas de leishmaniasis de las Américas: Ciudad de Panamá, 8 al 10 de octubre de 2013
  50. 2015, Guía para el abordaje integral de la leishmaniasis en Panamá, 2015

Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/50