Long-term endogenous acetylcholine deficiency potentiates pulmonary inflammation in a murine model of elastase-induced emphysema

Página do item simplificado
dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorBANZATO, Rosana
dc.contributor.authorPINHEIRO, Nathalia M.
dc.contributor.authorOLIVO, Clarice R.
dc.contributor.authorSANTANA, Fernanda R.
dc.contributor.authorLOPES, Fernanda D. T. Q. S.
dc.contributor.authorCAPERUTO, Luciana C.
dc.contributor.authorCAMARA, Niels O.
dc.contributor.authorMARTINS, Milton A.
dc.contributor.authorTIBERIO, Iolanda F. L. C.
dc.contributor.authorPRADO, Marco Antonio M.
dc.contributor.authorPRADO, Vania F.
dc.contributor.authorPRADO, Carla M.
dc.date.accessioned2021-10-20T13:55:48Z
dc.date.available2021-10-20T13:55:48Z
dc.date.issued2021
dc.description.abstractAcetylcholine (ACh), the neurotransmitter of the cholinergic system, regulates inflammation in several diseases including pulmonary diseases. ACh is also involved in a non-neuronal mechanism that modulates the innate immune response. Because inflammation and release of pro-inflammatory cytokines are involved in pulmonary emphysema, we hypothesized that vesicular acetylcholine transport protein (VAChT) deficiency, which leads to reduction in ACh release, can modulate lung inflammation in an experimental model of emphysema. Mice with genetical reduced expression of VAChT (VAChT KDHOM 70%) and wild-type mice (WT) received nasal instillation of 50 uL of porcine pancreatic elastase (PPE) or saline on day 0. Twenty-eight days after, animals were evaluated. Elastase instilled VAChT KDHOM mice presented an increase in macrophages, lymphocytes, and neutrophils in bronchoalveolar lavage fluid and MAC2-positive macrophages in lung tissue and peribronchovascular area that was comparable to that observed in WT mice. Conversely, elastase instilled VAChT KDHOM mice showed significantly larger number of NF-kappa B-positive cells and isoprostane staining in the peribronchovascular area when compared to elastase-instilled WT-mice. Moreover, elastase-instilled VAChT-deficient mice showed increased MCP-1 levels in the lungs. Other cytokines, extracellular matrix remodeling, alveolar enlargement, and lung function were not worse in elastase-instilled VAChT deficiency than in elastase-instilled WT-controls. These data suggest that decreased VAChT expression may contribute to the pathogenesis of emphysema, at least in part, through NF-kappa B activation, MCP-1, and oxidative stress pathways. This study highlights novel pathways involved in lung inflammation that may contribute to the development of chronic obstrutive lung disease (COPD) in cholinergic deficient individuals such as Alzheimer's disease patients.eng
dc.description.indexMEDLINEeng
dc.description.sponsorshipFundacAo de Amparo a Pesquisa do Estado de SAo PauloFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/15817-7, 2013/02881-4, 2018/15738-9, 2018/02537-5, 2018/06088-0, 2008/55359-5]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e TecnologicoConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [303035/2018-8]
dc.description.sponsorshipInstituto dos Laboratorios de InvestigacAo Medica do Hospital das Clinicas da Faculdade de Medicina da Universidade de SAo Paulo, Brazil [LIM-20-HC/FMUSP]
dc.identifier.citationSCIENTIFIC REPORTS, v.11, n.1, article ID 15918, 13p, 2021
dc.identifier.doi10.1038/s41598-021-95211-3
dc.identifier.issn2045-2322
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/42043
dc.language.isoeng
dc.publisherNATURE PORTFOLIOeng
dc.relation.ispartofScientific Reports
dc.rightsopenAccesseng
dc.rights.holderCopyright NATURE PORTFOLIOeng
dc.subject.othernonneuronal cholinergic systemeng
dc.subject.otherfactor-kappa-beng
dc.subject.otherrespiratory mechanicseng
dc.subject.otherlungeng
dc.subject.otherdiseaseeng
dc.subject.otherpathogenesiseng
dc.subject.othernerveeng
dc.subject.othermiceeng
dc.subject.othertransportereng
dc.subject.othermacrophageeng
dc.subject.wosMultidisciplinary Scienceseng
dc.titleLong-term endogenous acetylcholine deficiency potentiates pulmonary inflammation in a murine model of elastase-induced emphysemaeng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.affiliation.countryCanadá
hcfmusp.affiliation.countryisoca
hcfmusp.author.externalPINHEIRO, Nathalia M.:Univ Fed Sao Paulo, Dept Biosci, Rua Silva Jardim 136, Santos, SP, Brazil
hcfmusp.author.externalSANTANA, Fernanda R.:Univ Fed Sao Paulo, Dept Med Nephrol, Sao Paulo, Brazil; Univ Fed Sao Paulo, Dept Biol Sci, Diadema, Brazil
hcfmusp.author.externalCAPERUTO, Luciana C.:Univ Fed Sao Paulo, Dept Biol Sci, Diadema, Brazil
hcfmusp.author.externalCAMARA, Niels O.:Univ Sao Paulo, Immunol, Sao Paulo, Brazil
hcfmusp.author.externalPRADO, Marco Antonio M.:Robarts Res Inst, Mol Med Grp, London, ON, Canada; Univ Western Ontario, Dept Physiol & Pharmacol, London, ON, Canada; Univ Western Ontario, Dept Anat & Cell Biol, London, ON, Canada
hcfmusp.author.externalPRADO, Vania F.:Robarts Res Inst, Mol Med Grp, London, ON, Canada; Univ Western Ontario, Dept Physiol & Pharmacol, London, ON, Canada; Univ Western Ontario, Dept Anat & Cell Biol, London, ON, Canada
hcfmusp.citation.scopus1
hcfmusp.contributor.author-fmusphcROSANA SOUZA BANZATO
hcfmusp.contributor.author-fmusphcCLARICE ROSA OLIVO
hcfmusp.contributor.author-fmusphcFERNANDA DEGOBBI TENORIO QUIRINO DOS SANTOS LOPES
hcfmusp.contributor.author-fmusphcMILTON DE ARRUDA MARTINS
hcfmusp.contributor.author-fmusphcIOLANDA DE FATIMA LOPES CALVO TIBERIO
hcfmusp.contributor.author-fmusphcCARLA MAXIMO PRADO
hcfmusp.description.articlenumber15918
hcfmusp.description.issue1
hcfmusp.description.volume11
hcfmusp.origemWOS
hcfmusp.origem.pubmed34354132
hcfmusp.origem.scopus2-s2.0-85112004253
hcfmusp.origem.wosWOS:000684434700033
hcfmusp.publisher.cityBERLINeng
hcfmusp.publisher.countryGERMANYeng
hcfmusp.relation.referenceAgusti A, 2012, AM J RESP CRIT CARE, V185, P1171, DOI 10.1164/rccm.201203-0505UPeng
hcfmusp.relation.referenceAnciaes AM, 2011, CLINICS, V66, P1797, DOI 10.1590/S1807-59322011001000020eng
hcfmusp.relation.referenceAngeli P, 2008, AM J PHYSIOL-LUNG C, V294, pL1197, DOI 10.1152/ajplung.00199.2007eng
hcfmusp.relation.referenceBaggio C, 2020, J MED CHEM, V63, P12911, DOI 10.1021/acs.jmedchem.0c01285eng
hcfmusp.relation.referenceBarnes PJ, 2006, LAB INVEST, V86, P867, DOI 10.1038/labinvest.3700456eng
hcfmusp.relation.referenceBarnes PJ, 1997, NEW ENGL J MED, V336, P1066, DOI 10.1056/NEJM199704103361506eng
hcfmusp.relation.referenceBelmonte Kristen E, 2005, Proc Am Thorac Soc, V2, P297, DOI 10.1513/pats.200504-043SReng
hcfmusp.relation.referenceBorovikova LV, 2000, NATURE, V405, P458, DOI 10.1038/35013070eng
hcfmusp.relation.referenceBracke Ken R., 2007, Inflammation & Allergy Drug Targets, V6, P75eng
hcfmusp.relation.referenceBrasier AR, 2018, EXPERT REV RESP MED, V12, P931, DOI 10.1080/17476348.2018.1526677eng
hcfmusp.relation.referenceBudulac SE, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0033386eng
hcfmusp.relation.referenceCeylan E, 2006, RESP MED, V100, P1270, DOI 10.1016/j.rmed.2005.10.011eng
hcfmusp.relation.referenceChung KF, 2008, EUR RESPIR J, V31, P1334, DOI 10.1183/09031936.00018908eng
hcfmusp.relation.referenceChung KF, 2001, EUR RESPIR J, V18, p50S, DOI 10.1183/09031936.01.00229701eng
hcfmusp.relation.referenceCruz FF, 2012, RESP PHYSIOL NEUROBI, V182, P26, DOI 10.1016/j.resp.2012.01.002eng
hcfmusp.relation.referencede Boer WI, 2000, J PATHOL, V190, P619, DOI 10.1002/(SICI)1096-9896(200004)190:5<619::AID-PATH555>3.0.CO;2-6eng
hcfmusp.relation.referencede Castro BM, 2009, MOL CELL BIOL, V29, P5238, DOI 10.1128/MCB.00245-09eng
hcfmusp.relation.referenceDi Stefano A, 2002, EUR RESPIR J, V20, P556, DOI 10.1183/09031936.02.00272002eng
hcfmusp.relation.referenceDouaoui S, 2020, IMMUNOBIOLOGY, V225, DOI 10.1016/j.imbio.2020.151950eng
hcfmusp.relation.referenceFavier M, 2020, J CLIN INVEST, V130, P6616, DOI 10.1172/JCI138532eng
hcfmusp.relation.referenceFuke S, 2004, AM J RESP CELL MOL, V31, P405, DOI 10.1165/rcmb.2004-0131OCeng
hcfmusp.relation.referenceGallowitsch-Puerta M, 2005, ANN NY ACAD SCI, V1062, P209, DOI 10.1196/annals.1358.024eng
hcfmusp.relation.referenceGallowitsch-Puerta M, 2007, LIFE SCI, V80, P2325, DOI 10.1016/j.lfs.2007.01.002eng
hcfmusp.relation.referenceGeraghty P, 2013, FRONT PHYSIOL, V4, DOI 10.3389/fphys.2013.00267eng
hcfmusp.relation.referenceGessner C, 2005, RESP MED, V99, P1229, DOI 10.1016/j.rmed.2005.02.041eng
hcfmusp.relation.referenceGOLD, 2020, G I F C O L D GLOB Ieng
hcfmusp.relation.referenceGosens R, 2006, RESP RES, V7, DOI 10.1186/1465-9921-7-73eng
hcfmusp.relation.referenceGuzman MS, 2011, PLOS BIOL, V9, DOI 10.1371/journal.pbio.1001194eng
hcfmusp.relation.referenceGwilt CR, 2007, PHARMACOL THERAPEUT, V115, P208, DOI 10.1016/j.pharmthera.2007.05.007eng
hcfmusp.relation.referenceHacievliyagil SS, 2013, NIGER J CLIN PRACT, V16, P76, DOI 10.4103/1119-3077.106771eng
hcfmusp.relation.referenceHacievliyagil SS, 2006, RESP MED, V100, P846, DOI 10.1016/j.rmed.2005.08.022eng
hcfmusp.relation.referenceHantos Z, 2008, J APPL PHYSIOL, V105, P1864, DOI 10.1152/japplphysiol.90924.2008eng
hcfmusp.relation.referenceHogg JC, 2004, NEW ENGL J MED, V350, P2645, DOI 10.1056/NEJMoa032158eng
hcfmusp.relation.referenceIho S, 2003, J LEUKOCYTE BIOL, V74, P942, DOI 10.1189/jlb.1202626eng
hcfmusp.relation.referenceIto JT, 2019, CELLS-BASEL, V8, DOI 10.3390/cells8040342eng
hcfmusp.relation.referenceKanazawa H, 2005, CHEST, V128, P3191, DOI 10.1378/chest.128.5.3191eng
hcfmusp.relation.referenceKolisnyk B, 2013, J NEUROSCI, V33, P14908, DOI 10.1523/JNEUROSCI.1933-13.2013eng
hcfmusp.relation.referenceKolisnyk B, 2013, J NEUROSCI, V33, P10427, DOI 10.1523/JNEUROSCI.0395-13.2013eng
hcfmusp.relation.referenceKononov S, 2001, AM J RESP CRIT CARE, V164, P1920, DOI 10.1164/ajrccm.164.10.2101083eng
hcfmusp.relation.referenceKummer W, 2008, HISTOCHEM CELL BIOL, V130, P219, DOI 10.1007/s00418-008-0455-2eng
hcfmusp.relation.referenceLancas T, 2006, J APPL PHYSIOL, V100, P1610, DOI 10.1152/japplphysiol.00828.2005eng
hcfmusp.relation.referenceLara A, 2010, MOL CELL BIOL, V30, P1746, DOI 10.1128/MCB.00996-09eng
hcfmusp.relation.referenceLima RD, 2010, J NEUROCHEM, V113, P943, DOI 10.1111/j.1471-4159.2010.06657.xeng
hcfmusp.relation.referenceLopes FDTQS, 2009, ENVIRON RES, V109, P544, DOI 10.1016/j.envres.2009.03.002eng
hcfmusp.relation.referenceMacNee William, 2005, Proc Am Thorac Soc, V2, P258, DOI 10.1513/pats.200504-045SReng
hcfmusp.relation.referenceMacNee William, 2009, Proc Am Thorac Soc, V6, P527, DOI 10.1513/pats.200905-027DSeng
hcfmusp.relation.referenceManoury Boris, 2006, J Inflamm (Lond), V3, P2, DOI 10.1186/1476-9255-3-2eng
hcfmusp.relation.referenceMARGRAF LR, 1991, AM REV RESPIR DIS, V143, P391eng
hcfmusp.relation.referenceMartins-Olivera BT, 2016, MEDIAT INFLAMM, V2016, DOI 10.1155/2016/5346574eng
hcfmusp.relation.referenceMontuschi P, 1999, EUR J PHARMACOL, V365, P59, DOI 10.1016/S0014-2999(98)00859-0eng
hcfmusp.relation.referenceMonzon ME, 2011, AM J PHYSIOL-LUNG C, V300, pL204, DOI 10.1152/ajplung.00292.2010eng
hcfmusp.relation.referenceNeumann S, 2007, LIFE SCI, V80, P2361, DOI 10.1016/j.lfs.2007.01.010eng
hcfmusp.relation.referenceOenema TA, 2010, RESP RES, V11, DOI 10.1186/1465-9921-11-130eng
hcfmusp.relation.referencePavlov VA, 2006, BIOCHEM SOC T, V34, P1037, DOI 10.1042/BST0341037eng
hcfmusp.relation.referencePera T, 2011, EUR RESPIR J, V38, P789, DOI 10.1183/09031936.00146610eng
hcfmusp.relation.referencePereira MR, 2016, J CELL PHYSIOL, V231, P1862, DOI 10.1002/jcp.25307eng
hcfmusp.relation.referencePinheiro NM, 2020, EUR J PHARMACOL, V882, DOI 10.1016/j.ejphar.2020.173239eng
hcfmusp.relation.referencePinheiro NM, 2017, FASEB J, V31, P320, DOI 10.1096/fj.201600431Reng
hcfmusp.relation.referencePinheiro NM, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0120441eng
hcfmusp.relation.referencePrado MAM, 2002, NEUROCHEM INT, V41, P291, DOI 10.1016/S0197-0186(02)00044-Xeng
hcfmusp.relation.referencePrado VF, 2006, NEURON, V51, P601, DOI 10.1016/j.neuron.2006.08.005eng
hcfmusp.relation.referenceRosas-Ballina M, 2009, J INTERN MED, V265, P663, DOI 10.1111/j.1365-2796.2009.02098.xeng
hcfmusp.relation.referenceRosas-Ballina M, 2011, SCIENCE, V334, P98, DOI 10.1126/science.1209985eng
hcfmusp.relation.referenceRoy A, 2013, FASEB J, V27, P5072, DOI 10.1096/fj.13-238279eng
hcfmusp.relation.referenceSales DS, 2017, COPD, V14, P533, DOI 10.1080/15412555.2017.1346069eng
hcfmusp.relation.referenceSantana FPR, 2019, ECOTOX ENVIRON SAFE, V167, P494, DOI 10.1016/j.ecoenv.2018.10.005eng
hcfmusp.relation.referenceSchuliga M, 2015, BIOMOLECULES, V5, P1266, DOI 10.3390/biom5031266eng
hcfmusp.relation.referenceShapiro SD, 2003, EUR RESPIR J, V22, p30S, DOI 10.1183/09031936.03.00000903aeng
hcfmusp.relation.referenceShapiro SD, 1999, AM J RESP CRIT CARE, V160, pS29, DOI 10.1164/ajrccm.160.supplement_1.9eng
hcfmusp.relation.referenceSharafkhaneh Amir, 2008, Proc Am Thorac Soc, V5, P475, DOI 10.1513/pats.200708-126ETeng
hcfmusp.relation.referenceSOZZANI S, 1994, J IMMUNOL, V152, P3615eng
hcfmusp.relation.referenceSu X, 2010, J IMMUNOL, V184, P401, DOI 10.4049/jimmunol.0901808eng
hcfmusp.relation.referenceTaraseviciene-Stewart L, 2008, J CLIN INVEST, V118, P394, DOI 10.1172/JCI31811eng
hcfmusp.relation.referenceTracey KJ, 2007, J CLIN INVEST, V117, P289, DOI 10.1172/JCI30555eng
hcfmusp.relation.referenceVacca G, 2011, RESP RES, V12, DOI 10.1186/1465-9921-12-24eng
hcfmusp.relation.referenceWang H, 2003, NATURE, V421, P384, DOI 10.1038/nature01339eng
hcfmusp.relation.referenceWright JL, 2008, AM J PHYSIOL-LUNG C, V295, pL1, DOI [10.1152/ajplung.90200.2008, DOI 10.1152/ajplung.90200.2008]eng
hcfmusp.relation.referenceZhang J, 2011, RESP RES, V12, DOI 10.1186/1465-9921-12-158eng
hcfmusp.scopus.lastupdate2024-04-12
relation.isAuthorOfPublication643b5bc9-7fc1-4c0a-9f80-cb83d285489a
relation.isAuthorOfPublicationd8d1a306-c297-43b4-882e-3ec201f6eaa3
relation.isAuthorOfPublicationd40386e8-9a12-4131-908b-12a3c473cace
relation.isAuthorOfPublication30b65ddb-f92d-4fb7-8538-32ab9bed7597
relation.isAuthorOfPublication222fe0c7-b60e-42e9-8b03-3de63d2d1192
relation.isAuthorOfPublication948061e8-b5e0-414c-b402-cbc4878b2ba0
relation.isAuthorOfPublication.latestForDiscovery643b5bc9-7fc1-4c0a-9f80-cb83d285489a
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
art_BANZATO_Longterm_endogenous_acetylcholine_deficiency_potentiates_pulmonary_inflammation_in_2021.PDF
Tamanho:
3.64 MB
Formato:
Adobe Portable Document Format
Descrição:
publishedVersion (English)
Baixar
Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/05
Artigos e Materiais de Revistas Científicas - LIM/20
Artigos e Materiais de Revistas Científicas - ODS/03