Increased Levels of Circulating Endothelial Progenitor Cells in Human T-cell Lymphotropic Virus Type I Carriers
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Citações na Scopus
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Tipo de produção
article
Data de publicação
2011
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER SCIENCE INC
Autores
MEIRELES, Ana Luisa L. Pedroso
HALLACK NETO, Abrahao Elias
Citação
ARCHIVES OF MEDICAL RESEARCH, v.42, n.1, p.34-37, 2011
Resumo
Background and Aims. HTLV-I-transformed T cells secrete biologically active forms of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF). In addition, HTLV-I-transformed cells have a high capacity of adhesion to endothelial cells. Methods. We measured the circulating endothelial progenitor cells (EPCs) and mature endothelial cells (MECs) by flow cytometry in 27 HTLV-I carriers in comparison to 30 healthy, age- and gender-matched subjects. All subjects had HTLV-I positivity confirmed by Western blot and/or polymerase chain reaction (PCR). The numbers of different subpopulations of EPCs and MECSs were evaluated by four-color flow cytometry using a panel of monoclonal antibodies. All reactions were done in duplicate to confirm reproducibility of the results. Results. The median age of all 27 HTLV-I carriers enrolled in this study was 45 years (range: 27-65 years); 11(41%) were male and 16 (59%) were female. The median age of the 30 healthy subjects in the control group was 45.5 years (range: 20-63 years); 11 (36.6%) were male and 19 (63.4%) were female. The number of EPCs was significantly higher in HTLV-I carriers (median 0.8288 cells/mu L, range: 0.0920-3.3176 cells/mu L) as compared to control group (median 0.4905 cells/mu L, range: 0.0000-1.5660 cells/mu L) (p = 0.035). In contrast, the median of the MECs in the HTLV-I carriers was 0.6380 cells/mu L (range: 0.0473-5.7618 cells/mu L) and 0.4950 cells/mu L (range: 0.0000-4.0896 cells/mu L) in the control group, with no statistical difference (p = 0.697). Conclusions. We demonstrated that EPCs, but not MECs, are increased in the peripheral blood of HTLV-I carriers. (C) 2011 IMSS.
Palavras-chave
Flow cytometry, HTLV-I, Adult T-cell leukemia-lymphoma, Circulating endothelial cell
Referências
- Delorme B, 2005, THROMB HAEMOSTASIS, V94, P1270, DOI 10.1160/TH05-07-0499
- El-Sabban ME, 2002, BLOOD, V99, P3383, DOI 10.1182/blood.V99.9.3383
- Mori N, 2002, BLOOD, V99, P1341, DOI 10.1182/blood.V99.4.1341
- Proietti FA, 2005, ONCOGENE, V24, P6058, DOI 10.1038/sj.onc.1208968
- Ingram DA, 2005, BLOOD, V106, P1525, DOI 10.1182/blood-2005-04-1509
- Khan SS, 2005, CYTOM PART B-CLIN CY, V64B, P1, DOI 10.1002/cyto.b.20040
- Rafii S, 2002, NAT REV CANCER, V2, P826, DOI 10.1038/nrc925
- POIESZ BJ, 1980, P NATL ACAD SCI-BIOL, V77, P7415, DOI 10.1073/pnas.77.12.7415
- Mancuso P, 2001, BLOOD, V97, P3658, DOI 10.1182/blood.V97.11.3658
- Bazarbachi A, 2004, CANCER RES, V64, P2039, DOI 10.1158/0008-5472.CAN-03-2390
- GRETE A, 1993, J INFECT DIS, V167, P270
- Kchour G, 2008, LEUKEMIA LYMPHOMA, V49, P265, DOI 10.1080/10428190701760060
- Wierzbowska A, 2005, EUR J HAEMATOL, V75, P492, DOI 10.1111/j.1600-0609.2005.00549.x
- YOSHIDA M, 1982, P NATL ACAD SCI-BIOL, V79, P2031, DOI 10.1073/pnas.79.6.2031