Comparative evaluation of the DPP (R) CVL rapid test for canine serodiagnosis in area of visceral leishmaniasis

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorLAURENTI, M. D.
dc.contributor.authorLEANDRO JR., M. V. de Santana
dc.contributor.authorTOMOKANE, T. Y.
dc.contributor.authorLUCCA, H. R. L. De
dc.contributor.authorASCHAR, M.
dc.contributor.authorSOUZA, C. S. F.
dc.contributor.authorSILVA, R. M.
dc.contributor.authorMARCONDES, M.
dc.contributor.authorMATTA, V. L. R. da
dc.date.accessioned2015-04-22T22:16:39Z
dc.date.available2015-04-22T22:16:39Z
dc.date.issued2014
dc.description.abstractWe investigated the performance of the DPP (R) canine visceral leishmaniasis (CVL) rapid test, a novel immunochromatographic assay launched by BioManguinhos (Brazil), which was recently included in the new Brazilian protocol for screening CVL in serological surveys. The present study compared the DPP (R) with the ELISA and IFA produced by BioManguinhos (Brazil) both with L. major-like antigens and with in-house tests using Leishmania infantum chagasi (in-house ELISA and in-house IFA). We analyzed the sera from clinically symptomatic (n = 47) and asymptomatic (n = 38) infected dogs from an endemic area of CVL, as well as from healthy (n = 18) dogs, in addition to the sera of dogs (n = 81) infected with other pathogens. The DPP (R) and the in-house ELISA showed a sensitivity of 90.6% and 94.1%, respectively, and specificity of 95.1% and 97.5%, respectively, and both presented cross-reactivity only with the sera of dogs with babesiosis, 44% for the DPP (R) and 22% for the in-house ELISA. The clinical groups were detected equally by the two assays. The ELISA BioManguinhos, IFA BioManguinhos, and in house-IFA showed a good sensitivity, 90.6%, 96.5% and 89.4%, respectively, but very low specificity, 77.8%, 69.1% and 65.8%, respectively, due to the high cross-reactivity with the sera from the animals harboring other pathogens. The in-house ELISA provided the highest accuracy (95.8%), followed by the DPP (R) (92.7%), ELISA BioManguinhos (84.3%), IFA BioManguinhos (83.1%), and in-house IFA (78.0%). The simultaneous use of the DPP (R) and ELISA BioManguinhos reached a sensitivity of 99.1% and 82.1% when used sequentially. In conclusion, the DPP (R) performed well as serological test for CVL, and detected both asymptomatic and symptomatic dogs in equal proportions. Although its sensitivity is not ideal yet, discarding the IFA and including the DPP (R) improved the accuracy of the new Brazilian CVL diagnostic protocol, particularly of detecting truly infected dogs. Moreover, considering the higher specificity of DPP (R) (95.1% vs 77.8%), positive predictive value (95.1% vs 81.1%) and positive likelihood value (18.3% vs 4.1%) in comparison with the ELISA BioManguinhos, the use of DPP (R) as a confirmatory test instead of a screening test is suggested.
dc.description.indexMEDLINE
dc.description.sponsorshipSao Paulo Research Foundation [2004/07965-2, 2009/54533-4]
dc.description.sponsorship[LIM50 HC-FMUSP]
dc.identifier.citationVETERINARY PARASITOLOGY, v.205, n.3-4, p.444-450, 2014
dc.identifier.doi10.1016/j.vetpar.2014.09.002
dc.identifier.eissn1873-2550
dc.identifier.issn0304-4017
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/9082
dc.language.isoeng
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofVeterinary Parasitology
dc.rightsrestrictedAccess
dc.rights.holderCopyright ELSEVIER SCIENCE BV
dc.subjectCanine leishmaniasis
dc.subjectSerological diagnosis
dc.subjectEnzyme-linked immunosorbent assay (ELISA)
dc.subjectIndirect fluorescence test (IFA)
dc.subjectDPP (R) CVL rapid test
dc.subjectCross-reactivity
dc.subject.otherlinked immunosorbent assays
dc.subject.otherinfantum chagasi
dc.subject.otherclinical signs
dc.subject.otherdogs
dc.subject.otherdiagnosis
dc.subject.otherinfections
dc.subject.othertransmission
dc.subject.otherantigens
dc.subject.otherbrazil
dc.subject.wosParasitology
dc.subject.wosVeterinary Sciences
dc.titleComparative evaluation of the DPP (R) CVL rapid test for canine serodiagnosis in area of visceral leishmaniasis
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalLUCCA, H. R. L. De:Adolfo Lutz Inst, Rio Claro, SP, Brazil
hcfmusp.author.externalSOUZA, C. S. F.:Fiocruz MS, Inst Oswaldo Cruz, BR-21045900 Rio De Janeiro, RJ, Brazil
hcfmusp.author.externalSILVA, R. M.:Adolfo Lutz Inst, Rio Claro, SP, Brazil
hcfmusp.author.externalMARCONDES, M.:UNESP, Sch Vet, Dept Clin, Aracatuba, SP, Brazil
hcfmusp.citation.scopus60
hcfmusp.contributor.author-fmusphcMARCIA DALASTRA LAURENTI
hcfmusp.contributor.author-fmusphcMARCOS VINICIUS DE SANTANA LEANDRO JUNIOR
hcfmusp.contributor.author-fmusphcTHAISE YUMIE TOMOKANE
hcfmusp.contributor.author-fmusphcMARIANA ASCHAR FERRAZ
hcfmusp.contributor.author-fmusphcVANIA LUCIA RIBEIRO DA MATTA
hcfmusp.description.beginpage444
hcfmusp.description.endpage450
hcfmusp.description.issue3-4
hcfmusp.description.volume205
hcfmusp.origemWOS
hcfmusp.origem.pubmed25257505
hcfmusp.origem.scopus2-s2.0-84926181267
hcfmusp.origem.wosWOS:000344425200004
hcfmusp.publisher.cityAMSTERDAM
hcfmusp.publisher.countryNETHERLANDS
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