Microsatellite loci cross-species transferability in Aedes fluviatilis (Diptera:Culicidae): a cost-effective approach for population genetics studies

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorMULTINI, Laura Cristina
dc.contributor.authorMARRELLI, Mauro Toledo
dc.contributor.authorWILKE, Andre Barretto Bruno
dc.date.accessioned2016-02-11T14:32:10Z
dc.date.available2016-02-11T14:32:10Z
dc.date.issued2015
dc.description.abstractBackground: Aedes fluviatilis is a neotropical mosquito species thought to be a potential vector of Yellow Fever viruses and can be infected with Plasmodium gallinaceum in laboratory. A better understanding of its genetic structure is very important to understand its epidemiologic potential and how it is responding to urbanization. The objective of this study was to survey the transferability of microsatellites loci developed for other Aedes to Ae. fluviatilis. Findings: We tested in Ae. fluviatilis 40 pairs of primers known to flank microsatellite regions in Aedes aegypti, Aedes albopictus and Aedes caspius, and found eight loci that amplified consistently. The number of alleles per locus ranged from 2 to 15, and the expected heterozygosity ranged from 0.09 to 0.85. Conclusions: We found that several microsatellite primers successfully transferred to Ae. fluviatilis. This finding opens avenues for cost-effective optimization of high-resolution population genetic tools.
dc.description.indexMEDLINE
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2013/15313-4]
dc.description.sponsorshipFAPESP [2012/19117-2, 2013/22347-2]
dc.identifier.citationPARASITES & VECTORS, v.8, article ID 635, 4p, 2015
dc.identifier.doi10.1186/s13071-015-1256-9
dc.identifier.issn1756-3305
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/12931
dc.language.isoeng
dc.publisherBIOMED CENTRAL LTD
dc.relation.ispartofParasites & Vectors
dc.rightsopenAccess
dc.rights.holderCopyright BIOMED CENTRAL LTD
dc.subjectAedes fluviatilis
dc.subjectMicrosatellite
dc.subjectCulicidae
dc.subjectGenetic structure
dc.subject.otherculicidae populations
dc.subject.otherdiptera-culicidae
dc.subject.othersao-paulo
dc.subject.otheraegypti
dc.subject.othermarkers
dc.subject.othervector
dc.subject.othervariability
dc.subject.othersoftware
dc.subject.otherdengue
dc.subject.otherbrazil
dc.subject.wosParasitology
dc.titleMicrosatellite loci cross-species transferability in Aedes fluviatilis (Diptera:Culicidae): a cost-effective approach for population genetics studies
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalMARRELLI, Mauro Toledo:Univ Sao Paulo, Inst Trop Med Sao Paulo, BR-05403000 Sao Paulo, SP, Brazil; Univ Sao Paulo, Sch Publ Hlth, Dept Epidemiol, BR-01246904 Sao Paulo, SP, Brazil
hcfmusp.author.externalWILKE, Andre Barretto Bruno:Univ Sao Paulo, Sch Publ Hlth, Dept Epidemiol, BR-01246904 Sao Paulo, SP, Brazil
hcfmusp.citation.scopus9
hcfmusp.description.articlenumber635
hcfmusp.description.volume8
hcfmusp.origemWOS
hcfmusp.origem.pubmed26667177
hcfmusp.origem.scopus2-s2.0-84949813501
hcfmusp.origem.wosWOS:000366559600003
hcfmusp.publisher.cityLONDON
hcfmusp.publisher.countryENGLAND
hcfmusp.relation.referenceChambers EW, 2007, J HERED, V98, P202, DOI 10.1093/jhered/esm015
hcfmusp.relation.referencePorretta D, 2006, MOL ECOL NOTES, V6, P880, DOI 10.1111/j.1471-8286.2006.01384.x
hcfmusp.relation.referenceHuber K, 2001, MOL ECOL NOTES, V1, P219, DOI 10.1046/j.1471-8278 .2001.00077.x
hcfmusp.relation.referencePorretta D, 2005, MOL ECOL NOTES, V5, P48, DOI 10.1111/j.1471-8286.2004.00826.x
hcfmusp.relation.referenceCarlsson J, 2008, J HERED, V99, P616, DOI 10.1093/jhered/esn048
hcfmusp.relation.referenceBello F, 2009, GENET MOL RES, V8, P1179, DOI 10.4238/vol8-3gmr652
hcfmusp.relation.referenceEdillo FE, 2007, J MED ENTOMOL, V44, P145, DOI 10.1603/0022-2585(2007)44[145:ASOBAM]2.0.CO;2
hcfmusp.relation.referenceMedeiros-Sousa AR, 2013, J AM MOSQUITO CONTR, V29, P275, DOI 10.2987/12-6304R.1
hcfmusp.relation.referenceLi B, 2013, GENETICS, V195, P563, DOI 10.1534/genetics.113.154161
hcfmusp.relation.referenceRousset F, 2008, MOL ECOL RESOUR, V8, P103, DOI 10.1111/j.1471-8286.2007.01931.x
hcfmusp.relation.referenceToth G, 2000, GENOME RES, V10, P967, DOI 10.1101/gr.10.7.967
hcfmusp.relation.referenceMoreira LA, 2009, CELL, V139, P1268, DOI 10.1016/j.cell.2009.11.042
hcfmusp.relation.referenceBeebe NW, 2013, PLOS NEGLECT TROP D, V7, DOI 10.1371/journal.pntd.0002361
hcfmusp.relation.referenceBelisario CJ, 2015, PARASITE VECTOR, V8, DOI 10.1186/s13071-015-0879-1
hcfmusp.relation.referenceCarvalho G.C., 2014, J VECTOR ECOL, V39, P146
hcfmusp.relation.referenceCeretti W, 2015, J AM MOSQUITO CONTR, V31, P172, DOI 10.2987/14-6457R
hcfmusp.relation.referenceConsoli R.A.G.B., 1983, Memorias do Instituto Oswaldo Cruz, V78, P37
hcfmusp.relation.referenceDAVIS N. C., 1931, American Journal of Tropical Medicine, V11, P21
hcfmusp.relation.referencede Camargo M V, 1983, Mem Inst Oswaldo Cruz, V78, P83
hcfmusp.relation.referenceDelatte H, 2013, PLOS NEGLECT TROP D, V7, DOI 10.1371/journal.pntd.0002111
hcfmusp.relation.referenceExcoffier L, 2005, EVOL BIOINFORM, V1, P47
hcfmusp.relation.referenceMonteiro FA, 2014, PLOS NEGLECT TROP D, V8, DOI 10.1371/journal.pntd.0003167
hcfmusp.relation.referenceSamb B, 2012, PARASITE VECTOR, V5, DOI 10.1186/1756-3305-5-188
hcfmusp.relation.referenceWilke ABB, 2014, PARASITE VECTOR, V7, DOI 10.1186/s13071-014-0468-8
hcfmusp.relation.referenceWilkerson RC, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0133602
hcfmusp.relation.referenceWRBU. Walter Reed Biosystematics Unit, 2013, SYST CAT CUL
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