Comparative measurement of D- and L-lactic acid isomers in vaginal secretions: association with high-grade cervical squamous intraepithelial lesions

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorMAGALHAES, Christina Cordeiro Benevides de
dc.contributor.authorLINHARES, Iara Moreno
dc.contributor.authorMASULLO, Lais Farias
dc.contributor.authorELEUTERIO, Renata Mirian Nunes
dc.contributor.authorWITKIN, Steven S.
dc.contributor.authorJR, Jose Eleuterio
dc.date.accessioned2022-04-19T13:07:07Z
dc.date.available2022-04-19T13:07:07Z
dc.date.issued2022
dc.description.abstractPurpose Identification of low-cost protocols to identify women at elevated susceptibility to develop cervical intra-epithelial abnormalities would aid in more individualized monitoring. We evaluated whether quantitation of the D- and L-lactic acid isomers in vaginal secretions could differentiate women with normal cervical epithelia from those with a low (LSIL) or high (HSIL) grade squamous intraepithelial lesion or with cervical cancer. Methods Vaginal samples, collected from 78 women undetgoing cervical colposcopy and biopsy, were tested for pH, bacterial composition by Gram stain (Nugent score) and concentrations of D- and L-lactic acid by a colorimetric assay. Results Subsequent diagnosis was 23 women with normal cervical epithelium, 10 with LSIL, 43 with HSIL and 2 with cervical cancer. Vaginal pH and Nugent score were comparable in all subject groups. The concentration of L-lactic acid, but not D-lactic acid, as well as the L/D-lactic acid ratio, were significantly elevated (p < 0.01) in women with HSIL and cervical cancer. Conclusion Comparative measurement of vaginal D- and L-lactic acid isomers may provide a low-cost alternative to identification of women with an elevated susceptibility to cervical abnormalities.eng
dc.description.indexMEDLINEeng
dc.description.sponsorshipFederal University of Ceara
dc.identifier.citationARCHIVES OF GYNECOLOGY AND OBSTETRICS, v.305, n.2, p.373-377, 2022
dc.identifier.doi10.1007/s00404-021-06258-6
dc.identifier.eissn1432-0711
dc.identifier.issn0932-0067
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/46209
dc.language.isoeng
dc.publisherSPRINGER HEIDELBERGeng
dc.relation.ispartofArchives of Gynecology and Obstetrics
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright SPRINGER HEIDELBERGeng
dc.subjectLactic acid isomerseng
dc.subjectSquamous intraepithelial lesionseng
dc.subjectCervix uterieng
dc.subjectVaginaeng
dc.subject.otherhuman-papillomaviruseng
dc.subject.otherbacterial vaginosiseng
dc.subject.othermicrobiotaeng
dc.subject.wosObstetrics & Gynecologyeng
dc.titleComparative measurement of D- and L-lactic acid isomers in vaginal secretions: association with high-grade cervical squamous intraepithelial lesionseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.affiliation.countryEstados Unidos
hcfmusp.affiliation.countryisous
hcfmusp.author.externalMAGALHAES, Christina Cordeiro Benevides de:Univ Fed Ceara, Masters Program Pathol, Fortaleza, Ceara, Brazil
hcfmusp.author.externalMASULLO, Lais Farias:Univ Fed Ceara, Postgrad Program Dev & Technol Innovat Med, Fortaleza, Ceara, Brazil; UNICHRISTUS Univ Ctr, Fortaleza, Ceara, Brazil
hcfmusp.author.externalELEUTERIO, Renata Mirian Nunes:UNICHRISTUS Univ Ctr, Fortaleza, Ceara, Brazil; Prof Eleuterio Lab, Fortaleza, Ceara, Brazil
hcfmusp.author.externalWITKIN, Steven S.:Weill Cornell Med, Dept Obstet & Gynecol, New York, NY USA
hcfmusp.author.externalJR, Jose Eleuterio:Univ Fed Ceara, Masters Program Pathol, Fortaleza, Ceara, Brazil; Prof Eleuterio Lab, Fortaleza, Ceara, Brazil; Univ Fed Ceara, Fac Med, Bloco Didat,Rua Prof Costa Mendes 1608,2nd Floor, BR-60430140 Fortaleza, Ceara, Brazil
hcfmusp.citation.scopus0
hcfmusp.contributor.author-fmusphcIARA MORENO LINHARES
hcfmusp.description.beginpage373
hcfmusp.description.endpage377
hcfmusp.description.issue2
hcfmusp.description.volume305
hcfmusp.origemWOS
hcfmusp.origem.pubmed34554315
hcfmusp.origem.scopus2-s2.0-85115401144
hcfmusp.origem.wosWOS:000698553100001
hcfmusp.publisher.cityHEIDELBERGeng
hcfmusp.publisher.countryGERMANYeng
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