Molecular diagnosis of Strongyloides stercoralis among transplant candidates

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorPAULA, Fabiana M.
dc.contributor.authorMALTA, Fernanda M.
dc.contributor.authorMARQUES, Priscilla D.
dc.contributor.authorMELO, Gessica B.
dc.contributor.authorCORRAL, Marcelo A.
dc.contributor.authorGOTTARDI, Maiara
dc.contributor.authorPINHO, Joao R. R.
dc.contributor.authorGONCALVES, Elenice M. N.
dc.contributor.authorCASTILHO, Vera L. P.
dc.contributor.authorPIERROTTI, Ligia C.
dc.contributor.authorABDALA, Edson
dc.contributor.authorCOSTA, Silvia F.
dc.contributor.authorCHIEFFI, Pedro P.
dc.contributor.authorGRYSCHEK, Ronaldo C. B.
dc.date.accessioned2018-09-13T15:23:58Z
dc.date.available2018-09-13T15:23:58Z
dc.date.issued2018
dc.description.abstractStrongyloidiasis can occur without any symptoms or as a potentially fatal hyperinfection or disseminated infection, principally in immunosuppressed patients. Our study aimed to evaluate the application of conventional polymerase chain reaction (cPCR) and real-time PCR (qPCR). Polymerase chain reaction (PCR) and real-time PCR (qPCR) targeting the 18S rRNA gene for detection of Strongyloides stercoralis infection among transplant candidates were applied in stool samples obtained from 150 transplant candidates, preliminarily analyzed by parasitological methods. S.stercoralis larvae were visualized in 15/150 (10.0%) transplant candidates by parasitological methods. DNA from S.stercoralis was amplified in 26/150 (17.3%) and 49/150 (32.7%) stool samples of transplant candidates, using cPCR and qPCR, respectively. The results suggest that molecular methods, especially qPCR, should be used as an additional tool for diagnostic of S.stercoralis infection among transplant candidates.
dc.description.indexMEDLINE
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo [FAPESP 2010/51110-2]
dc.identifier.citationTRANSPLANT INFECTIOUS DISEASE, v.20, n.4, article ID e12909, 4p, 2018
dc.identifier.doi10.1111/tid.12909
dc.identifier.eissn1399-3062
dc.identifier.issn1398-2273
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/28129
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofTransplant Infectious Disease
dc.rightsrestrictedAccess
dc.rights.holderCopyright WILEY
dc.subjectmolecular diagnosis
dc.subjectStrongyloides stercoralis
dc.subjecttransplant candidate
dc.subject.otherreal-time pcr
dc.subject.otherhuman stool samples
dc.subject.otherinfection
dc.subject.otherhookworm
dc.subject.wosImmunology
dc.subject.wosInfectious Diseases
dc.subject.wosTransplantation
dc.titleMolecular diagnosis of Strongyloides stercoralis among transplant candidates
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalGOTTARDI, Maiara:Univ Sao Paulo, Hosp Clin,Fac Med, Lab Invest Med, LIM Lab Imunopatol Esquistossomose 06, Sao Paulo, Brazil
hcfmusp.author.externalCHIEFFI, Pedro P.:Fac Ciencias Med, Santa Casa, SP, Brazil
hcfmusp.citation.scopus8
hcfmusp.contributor.author-fmusphcFABIANA MARTINS DE PAULA
hcfmusp.contributor.author-fmusphcFERNANDA DE MELLO MALTA
hcfmusp.contributor.author-fmusphcPRISCILLA DUARTE MARQUES FONSECA
hcfmusp.contributor.author-fmusphcGESSICA BAPTISTA DE MELO
hcfmusp.contributor.author-fmusphcMARCELO ANDREETTA CORRAL
hcfmusp.contributor.author-fmusphcJOAO RENATO REBELLO PINHO
hcfmusp.contributor.author-fmusphcELENICE MESSIAS DO NASCIMENTO GONCALVES
hcfmusp.contributor.author-fmusphcVERA LUCIA PAGLIUSI CASTILHO
hcfmusp.contributor.author-fmusphcLIGIA CAMERA PIERROTTI
hcfmusp.contributor.author-fmusphcEDSON ABDALA
hcfmusp.contributor.author-fmusphcSILVIA FIGUEIREDO COSTA
hcfmusp.contributor.author-fmusphcRONALDO CESAR BORGES GRYSCHEK
hcfmusp.description.articlenumbere12909
hcfmusp.description.issue4
hcfmusp.description.volume20
hcfmusp.origemWOS
hcfmusp.origem.pubmed29679429
hcfmusp.origem.scopus2-s2.0-85052810148
hcfmusp.origem.wosWOS:000440417900019
hcfmusp.publisher.cityHOBOKEN
hcfmusp.publisher.countryUSA
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hcfmusp.scopus.lastupdate2024-05-10
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