DNA methylation pattern changes following a short-term hypocaloric diet in women with obesity

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorNICOLETTI, C. F.
dc.contributor.authorCORTES-OLIVEIRA, C.
dc.contributor.authorNORONHA, N. Y.
dc.contributor.authorPINHEL, M. A. S.
dc.contributor.authorDANTAS, W. S.
dc.contributor.authorJACOME, A.
dc.contributor.authorMARCHINI, J. S.
dc.contributor.authorGUALANO, B.
dc.contributor.authorCRUJEIRAS, A. B.
dc.contributor.authorNONINO, C. B.
dc.date.accessioned2020-10-15T14:30:50Z
dc.date.available2020-10-15T14:30:50Z
dc.date.issued2020
dc.description.abstractBackground/Objectives We aimed to investigate the effects of short-term hypocaloric diet-induced weight loss on DNA methylation profile in leukocytes from women with severe obesity. Methods Eleven women with morbid obesity (age: 36.9 +/- 10.3 years; BMI: 58.5 +/- 10.5 kg/m(2)) were assessed before and after 6 weeks of a hypocaloric dietary intervention. The participants were compared with women of average weight and the same age (age: 36.9 +/- 11.8 years; BMI: 22.5 +/- 1.6 kg/m(2)). Genome-wide DNA methylation analysis was performed in DNA extracted from peripheral blood leukocytes using the Infinium Human Methylation 450 BeadChip assay. Changes (Delta beta) in the methylation level of each CpGs were calculated. A threshold with a minimum value of 10%, p < 0.001, for the significant CpG sites based on Delta beta and a false discovery rate of Results Dietary intervention changed the methylation levels at 16,064 CpG sites. These CpGs sites were related to cancer, cell cycle-related, MAPK, Rap1, and Ras signaling pathways. However, regardless of hypocaloric intervention, a group of 878 CpGs (related to 649 genes) remained significantly altered in obese women when compared with normal-weight women. Pathway enrichment analysis identified genes related to the cadherin and Wnt pathway, angiogenesis signaling, and p53 pathways by glucose deprivation. Conclusion A short-term hypocaloric intervention in patients with severe obesity partially restored the obesity-related DNA methylation pattern. Thus, the full change of obesity-related DNA methylation patterns could be proportional to the weight-loss rate in these patients after dietary interventions.eng
dc.description.indexMEDLINEeng
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2016/06470-7, 2016/05638-1, 2015/186690]
dc.description.sponsorshipCentro de Investigacion Biomedica En Red (CIBERobn)
dc.description.sponsorshipInstituto de Salud Carlos III (ISCIII), SpainInstituto de Salud Carlos III [PI17/01287]
dc.description.sponsorshipEuropean Regional Development Fund (FEDER)European Union (EU)
dc.description.sponsorshipMINECO through the ERDF [MTM2014-52876-R, MTM2017-82724-R]
dc.description.sponsorshipXunta de Galicia (Grupos de Referencia Competitiva) through the ERDF [ED431C-2016-015]
dc.description.sponsorshipXunta de Galicia (Centro Singular de Investigacion de Galicia) through the ERDF [ED431G/01]
dc.description.sponsorshipISCII [CP17/00088]
dc.description.sponsorshipEuropean Regional Development FundEuropean Union (EU)
dc.identifier.citationEUROPEAN JOURNAL OF CLINICAL NUTRITION, v.74, n.9, p.1345-1353, 2020
dc.identifier.doi10.1038/s41430-020-0660-1
dc.identifier.eissn1476-5640
dc.identifier.issn0954-3007
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/37656
dc.language.isoeng
dc.publisherNATURE PUBLISHING GROUPeng
dc.relation.ispartofEuropean Journal of Clinical Nutrition
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright NATURE PUBLISHING GROUPeng
dc.subject.otherepigenome-wide associationeng
dc.subject.otherbody-mass indexeng
dc.subject.otherweight-losseng
dc.subject.otherafrican-americaneng
dc.subject.otheradipose-tissueeng
dc.subject.otherexpressioneng
dc.subject.otherpromotereng
dc.subject.othermuscleeng
dc.subject.otherbmieng
dc.subject.othermeneng
dc.subject.wosNutrition & Dieteticseng
dc.titleDNA methylation pattern changes following a short-term hypocaloric diet in women with obesityeng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.affiliation.countryEspanha
hcfmusp.affiliation.countryisoes
hcfmusp.author.externalNICOLETTI, C. F.:Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Hlth Sci, Lab Nutrigen Studies, Ribeirao Preto, Brazil; Univ Sao Paulo, Fac Med FMUSP, Sch Phys Educaton & Sport, Rheumatol Div,Appl Physiol & Nutr Res Grp, Sao Paulo, SP, Brazil
hcfmusp.author.externalCORTES-OLIVEIRA, C.:Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Hlth Sci, Lab Nutrigen Studies, Ribeirao Preto, Brazil
hcfmusp.author.externalNORONHA, N. Y.:Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Hlth Sci, Lab Nutrigen Studies, Ribeirao Preto, Brazil
hcfmusp.author.externalPINHEL, M. A. S.:Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Hlth Sci, Lab Nutrigen Studies, Ribeirao Preto, Brazil; Sao Jose do Rio Preto Med Sch, Dept Mol Biol, Lab Studies Biochem & Mol Biol, Sao Paulo, Brazil
hcfmusp.author.externalJACOME, A.:Univ A Coruna, Fac Sci, Dept Math, MODES Grp,CITIC, La Coruna, Spain
hcfmusp.author.externalMARCHINI, J. S.:Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, Ribeirao Preto, Brazil
hcfmusp.author.externalCRUJEIRAS, A. B.:Santiago de Compostela Univ USC, Complejo Hosp Univ Santiago CHUS, Inst Invest Sanitaria IDIS, Epigen Endocrinol & Nutr, Santiago De Compostela, Spain; CIBER Fisiopatol Obesidad & Nutr CIBERobn, Madrid, Spain
hcfmusp.author.externalNONINO, C. B.:Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Hlth Sci, Lab Nutrigen Studies, Ribeirao Preto, Brazil
hcfmusp.citation.scopus15
hcfmusp.contributor.author-fmusphcWAGNER SILVA DANTAS
hcfmusp.contributor.author-fmusphcBRUNO GUALANO
hcfmusp.description.beginpage1345
hcfmusp.description.endpage1353
hcfmusp.description.issue9
hcfmusp.description.volume74
hcfmusp.origemWOS
hcfmusp.origem.pubmed32404903
hcfmusp.origem.scopus2-s2.0-85084513846
hcfmusp.origem.wosWOS:000532634700006
hcfmusp.publisher.cityLONDONeng
hcfmusp.publisher.countryENGLANDeng
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