Comparative effect of FGF2, synthetic peptides 1-28 N-POMC and ACTH on proliferation in rat adrenal cell primary cultures

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art_MATTOS_Comparative_effect_of_FGF2_synthetic_peptides_128_NPOMC_2011.PDF (915.14 KB)
Citações na Scopus
18
Tipo de produção
article
Data de publicação
2011
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
SPRINGER
Autores
MATTOS, Gabriele E.
AMARANTE-MENDES, Gustavo P.
LOTFI, Claudimara Ferini Pacicco
Citação
CELL AND TISSUE RESEARCH, v.345, n.3, p.343-356, 2011
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
There is evidence that pro-opiomelanocortin (POMC)-derived peptides other than adrenocorticotropic hormone (ACTH) have a role in adrenal cell proliferation. We compared the activity of synthetic rat N-terminal POMC fragment 1-28 with disulfide bridges (N-POMC(w)) and without disulfide bridges (N-POMC(w/o)), with the activity of fibroblast growth factor (FGF2), a widely studied adrenal growth factor, and ACTH, in well-characterized pure cultures of both isolated adrenal Glomerulosa (G) and Fasciculata/Reticularis (F/R) cells. Three days of FGF2-treatment had a proliferative effect similar to serum, and synthetic peptide N-POMC(w) induced proliferation more efficiently than N-POMC(w/o). Moreover, both induced proliferation via the ERK1/2 pathway. In contrast, sustained ACTH treatment decreased proliferation and viability through apoptosis induction, but not necrosis, and independently of PKA and PKC pathways. Further elucidation of 1-28 POMC signal transduction is of interest, and primary cultures of adrenal cells were found to be useful for examining the trophic activity of this peptide.
Palavras-chave
Rat primary adrenal culture, 1-28 N-POMC, ACTH, FGF2, Proliferation, Rat (Sprague Dawley)
URI
https://observatorio.fm.usp.br/handle/OPI/24136
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Coleções
Artigos e Materiais de Revistas Científicas - LIM/62