Cytotoxicity of cashew flavonoids towards malignant cell lines
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Citações na Scopus
41
Tipo de produção
article
Data de publicação
2012
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER GMBH, URBAN & FISCHER VERLAG
Autores
KONAN, Nzi Andre
LINCOPAN, Nilton
DIAZ, Ingrit Elida Collantes
TIBA, Mirtes Midori Tanae
MENDES, Joao Gustavo Pessini Amarante
BACCHI, Elfriede Marianne
SPIRA, Beny
Citação
EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY, v.64, n.5, p.435-440, 2012
Resumo
The leaves of the Cashew plant (Anacardium occidentale L.) are used by the folk medicine in South America and West Africa. This plant is rich in flavonoids, which are polyphenolic compounds widespread in plants, and that have diverse physiological effects. In a sub-acute toxicity assay it was found that an ethanolic extract of Cashew leaves elicited lymphopenia in rats. The extract was also found to be cytotoxic and to induce apoptosis in Jurkat (acute lymphoblastic leukemia) cells. The crude ethanolic extract was fractionated and resolved by HPLC. One of the four fractions obtained led to the isolation of the biflavonoid agasthisflavone. [H-3]-thymidine incorporation assays and flow cytometry analysis showed that the isolated compound displayed a high anti-proliferative effect in Jurkat cells with an IC50 of 2.4 mu g/ml (4.45 mu M). The effect of agathisflavone on the acute promyelocytic leukemia cell line HL60, Burkitt lymphoma Raji cells and Hep-2 laryngeal carcinoma cells was also tested. The two latter ones were only mildly affected by agathisflavone. It is also shown that agathisflavone induces apoptosis in Jurkat cells and it this proposed that this is the likely mechanism of agathisflavone specific cytotoxicity.
Palavras-chave
Cashew, Rats, Leukemia cells
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