Quantification of urinary 5-hydroxyindoleacetic acid by in-house nitrosonaphthol reaction compared with nitrosonaphthol micro column chromatography and enzyme-linked immunosorbent assay

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorSILVA, Joyce Matie Kinoshita da
dc.contributor.authorMATTAR, Rejane
dc.contributor.authorCOLAÚTO, Carlos
dc.contributor.authorCARRILHO, Flair José
dc.date.accessioned2016-02-24T15:58:42Z
dc.date.available2016-02-24T15:58:42Z
dc.date.issued2014
dc.description.abstractThe aim of this study was to compare the colorimetric ""kit"" and enzyme-linked immunosorbent assay (ELISA) methods to quantify urinary 5-hydroxyindoleacetic acid through the Goldenberg's technique, exploring the potential of replacing it. 24-hour urine samples were tested by Goldenberg's assay and compared with kits. The agreement was almost perfect for the comparison of Goldenberg's assay with both colorimetric kit, and with ELISA kit, considering ≤ 7.5 mg/24h normal cutoff value. Therefore, both ""kits"" would be good alternatives to Goldenberg's technique due to practicality and agreement between values.
dc.description.abstractO objetivo deste estudo foi comparar métodos por kit colorimétrico e por ensaio imunossorvente ligado à enzima (ELISA) para quantificar o ácido 5-hidroxi-indolacético urinário com a técnica de Goldenberg, explorando o potencial de substituí-la. Amostras de urina de 24 horas foram testadas pela técnica de Goldenberg e com os kits. A concordância foi quase perfeita, tanto para a comparação do ensaio de Goldenberg com o kit colorimétrico quanto para com o kit ELISA, considerando normal o valor de corte de ≤ 7.5 mg/24h. Portanto, ambos os kits seriam boa alternativa para a técnica de Goldenberg devido à praticidade e à concordância entre os valores.
dc.description.indexPubMed
dc.identifier.citationJORNAL BRASILEIRO DE PATOLOGIA E MEDICINA LABORATORIAL, v.50, n.3, p.210-214, 2014
dc.identifier.doi10.5935/1676-2444.20140017
dc.identifier.issn1678-4774
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/13025
dc.language.isoeng
dc.publisherSociedade Brasileira de Patologia Clínica
dc.relation.ispartofJornal Brasileiro de Patologia e Medicina Laboratorial
dc.rightsopenAccess
dc.rights.holderCopyright Sociedade Brasileira de Patologia Clínica
dc.subject5-hydroxyindoleacetic acid
dc.subjectELISA
dc.subjectcarcinoid tumors
dc.subjectserotonin
dc.subjectnitrosonaphthol
dc.subjectácido 5-hidroxi-indolacético
dc.subjecttumores carcinoides
dc.subjectserotonina
dc.subjectnitrosonaftol
dc.subject.wosMedical Laboratory Technology
dc.subject.wosMedicine, Research & Experimental
dc.subject.wosPathology
dc.titleQuantification of urinary 5-hydroxyindoleacetic acid by in-house nitrosonaphthol reaction compared with nitrosonaphthol micro column chromatography and enzyme-linked immunosorbent assay
dc.title.alternativeQuantificação do ácido 5-hidroxi-indolacético urinário por técnica caseira do nitrosonaftol comparada com ensaio de nitrosonaftol em microcoluna de cromatografia e imunoensaio enzimático
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalCOLAÚTO, Carlos:Universidade de São Paulo, Brazil
hcfmusp.citation.scopus1
hcfmusp.contributor.author-fmusphcJOYCE MATIE KINOSHITA DA SILVA ETTO
hcfmusp.contributor.author-fmusphcREJANE MATTAR
hcfmusp.contributor.author-fmusphcFLAIR JOSE CARRILHO
hcfmusp.description.beginpage210
hcfmusp.description.endpage214
hcfmusp.description.issue3
hcfmusp.description.volume50
hcfmusp.origemsciELO
hcfmusp.origem.scieloSCIELO:S1676-24442014000300210
hcfmusp.origem.scopus2-s2.0-84940292833
hcfmusp.publisher.cityRio de Janeiro
hcfmusp.publisher.countryBRAZIL
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hcfmusp.relation.referenceGanetsky A, 2012, ANN PHARMACOTHER, V46, P851, DOI 10.1345/aph.1Q729
hcfmusp.relation.referenceGOLDENBE.H, 1973, CLIN CHEM, V19, P38
hcfmusp.relation.referenceHernandez R, 2008, AM J EMERG MED, V26, P282, DOI 10.1016/j.ajem.2007.05.012
hcfmusp.relation.referenceJangjoo A, 2012, AM J EMERG MED, V30, P540, DOI 10.1016/j.ajem.2011.01.027
hcfmusp.scopus.lastupdate2024-05-17
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