Human Synovial Mesenchymal Stem Cells Good Manufacturing Practices for Articular Cartilage Regeneration

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorFERNANDES, Tiago Lazzaretti
dc.contributor.authorKIMURA, Heitor Akio
dc.contributor.authorPINHEIRO, Carla Cristina Gomes
dc.contributor.authorSHIMOMURA, Kazunori
dc.contributor.authorNAKAMURA, Norimasa
dc.contributor.authorFERREIRA, Jose Ricardo
dc.contributor.authorGOMOLL, Andreas H.
dc.contributor.authorHERNANDEZ, Arnaldo Jose
dc.contributor.authorBUENO, Daniela Franco
dc.date.accessioned2019-01-17T13:35:03Z
dc.date.available2019-01-17T13:35:03Z
dc.date.issued2018
dc.description.abstractBackground: Cartilage restoration is a desperately needed bridge for patients with symptomatic cartilage lesions. Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. Despite autologous chondrocyte implantation versatility, it still fails to fully reproduce hyaline articular cartilage characteristics. Mesenchymal stem cells (MSCs) may be isolated from various known tissues, including discarded fragments at arthroscopy such as synovial membrane. Choice of harvesting site is motivated by MSCs' abilities to modulate immunologic and inflammatory response through paracrine communication. Synovial MSCs have a greater proliferation and strong chondrogenic potential than bone and adipose MSCs and a less hypertrophic differentiation than bone MSCs. Good manufacturing practice (GMP) laboratory techniques for human clinical trials are still novel. To our knowledge, there are only two clinical trials in humans published since today. Purpose: Therefore, this work aimed to isolate and characterize synovial MSCs and evaluated their differentiation properties according to GMP standards. Materials and Methods: One-gram tissue sample from three patients of synovia was harvested at the beginning of arthroscopy surgery. MSCs were isolated, expanded, and characterized by flow cytometry. Results: It was possible to isolate and expand MSCs cultures from synovia, characterize MSCs by flow cytometry using proper monoclonal antibodies, and differentiate MSCs by coloring technique after chondrogenic, adipogenic, and osteogenic differentiations. Cartilage treatment may benefit from these tissue engineering protocols since arthroscopic procedures are routinely performed for different purposes in a previous stage and a favorable chondronegic differentiation cell lineage may be collected and stored in a less invasive way. Conclusion: Laboratory protocols established according to presented GMP were able to isolate and characterize MSCs obtained from synovia.eng
dc.description.indexMEDLINEeng
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2017/05774-5]
dc.description.sponsorshipCo-ordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [88881.171651/2018-01]
dc.description.sponsorshipInternational Society of Arthroscopy, Knee Surgery and Orthopaedic Sports Medicine
dc.description.sponsorshipSirio-Libanes Hospital
dc.description.sponsorshipOrthopaedic Research and Education Foundation (ISAKOS Osteoarthritis)
dc.identifier.citationTISSUE ENGINEERING PART C-METHODS, v.24, n.12, p.709-716, 2018
dc.identifier.doi10.1089/ten.tec.2018.0219
dc.identifier.eissn1937-3392
dc.identifier.issn1937-3384
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/29996
dc.language.isoeng
dc.publisherMARY ANN LIEBERT, INCeng
dc.relation.ispartofTissue Engineering Part C-Methods
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright MARY ANN LIEBERT, INCeng
dc.subjectsynoviaeng
dc.subjecttissue engineeringeng
dc.subjectmesenchymal stem cellseng
dc.subjecthyaline articular cartilageeng
dc.subjectchondrogenic differentiationeng
dc.subjectimmune modulationeng
dc.subject.otherautologous chondrocyte implantationeng
dc.subject.otherosteogenic differentiationeng
dc.subject.otherfat padeng
dc.subject.otherkneeeng
dc.subject.otherrepaireng
dc.subject.otherdefectseng
dc.subject.othertherapyeng
dc.subject.otherhyaluronateeng
dc.subject.otherscaffoldeng
dc.subject.othermarkerseng
dc.subject.wosCell & Tissue Engineeringeng
dc.subject.wosBiotechnology & Applied Microbiologyeng
dc.subject.wosCell Biologyeng
dc.titleHuman Synovial Mesenchymal Stem Cells Good Manufacturing Practices for Articular Cartilage Regenerationeng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.affiliation.countryEstados Unidos
hcfmusp.affiliation.countryJapão
hcfmusp.affiliation.countryisojp
hcfmusp.affiliation.countryisous
hcfmusp.author.externalKIMURA, Heitor Akio:Hosp Sirio Libanes, Inst Ensino & Pesquisa, Sao Paulo, Brazil
hcfmusp.author.externalPINHEIRO, Carla Cristina Gomes:Hosp Sirio Libanes, Inst Ensino & Pesquisa, Sao Paulo, Brazil
hcfmusp.author.externalSHIMOMURA, Kazunori:Osaka Univ, Dept Orthopaed Surg, Grad Sch Med, Osaka, Japan
hcfmusp.author.externalNAKAMURA, Norimasa:Osaka Univ, Ctr Adv Med Engn & Informat, Osaka, Japan
hcfmusp.author.externalFERREIRA, Jose Ricardo:Mil Inst Engn IME, Dept Mat Sci, Post Grad Programme Mat Sci, Rio De Janeiro, Brazil
hcfmusp.author.externalGOMOLL, Andreas H.:Hosp Special Surg, Orthoped Surg & Sports Med, 535 E 70th St, New York, NY 10021 USA
hcfmusp.author.externalBUENO, Daniela Franco:Hosp Sirio Libanes, Inst Ensino & Pesquisa, Sao Paulo, Brazil
hcfmusp.citation.scopus33
hcfmusp.contributor.author-fmusphcTIAGO LAZZARETTI FERNANDES
hcfmusp.contributor.author-fmusphcARNALDO JOSE HERNANDEZ
hcfmusp.description.beginpage709
hcfmusp.description.endpage716
hcfmusp.description.issue12
hcfmusp.description.volume24
hcfmusp.origemWOS
hcfmusp.origem.pubmed30412046
hcfmusp.origem.scopus2-s2.0-85058519947
hcfmusp.origem.wosWOS:000453296700004
hcfmusp.publisher.cityNEW ROCHELLEeng
hcfmusp.publisher.countryUSAeng
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