GLYCOLIPID SENSING AND INNATE IMMUNITY TO PARACOCCIDIOIDOMYCOSIS

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorBATISTA, V. G.
dc.contributor.authorTOLEDO, M. S.
dc.contributor.authorSTRAUS, A. H.
dc.contributor.authorTAKAHASHI, H. K.
dc.contributor.authorVASCONCELOS, D. Moraes
dc.contributor.authorDUARTE, A. J.
dc.contributor.authorBENARD, G.
dc.date.accessioned2013-10-11T21:25:45Z
dc.date.available2013-10-11T21:25:45Z
dc.date.issued2012
dc.description.abstractParacoccidioidomycosis (PCM) is the most important systemic mycosis in Latin America. As in other endemic infectious diseases, only a minority of the exposed individuals will develop the disease; however, the mechanisms involved in susceptibility/resistance to PCM remain unknown. To address this we investigated some aspects of the innate immunity of 15 healthy individuals who have had PCM in the past and had been cured without sequels >10 years previously, representing the “susceptible” group, and 20 healthy individuals without PCM in the past (controls). For this we also used two glycolipids present in the fungus membrane, monohexosylceramide and glycosylinositol-phosphorylceramide, previously shown to exhibit immunological properties. There were significant lower expression of the costimulatory molecules CD86 and HLA-DR by susceptible individuals' monocytes and dendritic cells. Other costimulatory molecules such as CD80/CD40, PAMP receptors (TLR-4/TLR2/dectin-1) and CD1d expression were not different between the two groups. Both glycolipids further decrease CD86 and HLA-DR expression, while increased dectin-1 expression in both cell subsets. Monocytes and DCs' from susceptible individuals presented higher constitutive IL-10 production than control individuals, while TNF and IL-12 productions were comparable. DC ́s ability to induce lymphocyte proliferation to a fungal antigen was in general higher in controls than susceptible individuals; this DC function was suppressed by both glycolipids. Both glycolipids also blocked DC's ability to induce lymphocyte proliferation to P. brasiliensis immunodominant antigen. Subtle but significant differences in innate immunity functions and responses to P. brasiliensis glycolipids between “susceptible” and control individuals may underlie the mechanisms involved in resistance/susceptibility to paracoccidioidomycosis.
dc.description.conferencedateOCT 03-06, 2012
dc.description.conferencelocalFlorence, ITALY
dc.description.conferencename15th Biennial Meeting European-Society-for-Immunodeficiency (ESID)
dc.description.indexMEDLINE
dc.identifier.citationJOURNAL OF CLINICAL IMMUNOLOGY, v.32, suppl.1, p.395-396, 2012
dc.identifier.issn0271-9142
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/3010
dc.language.isoeng
dc.publisherSPRINGER/PLENUM PUBLISHERS
dc.relation.ispartofJournal of Clinical Immunology
dc.rightsrestrictedAccess
dc.rights.holderCopyright SPRINGER/PLENUM PUBLISHERS
dc.subject.wosImmunology
dc.titleGLYCOLIPID SENSING AND INNATE IMMUNITY TO PARACOCCIDIOIDOMYCOSIS
dc.typeconferenceObject
dc.type.categorymeeting abstract
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalBATISTA, V. G.:Univ Sao Paulo, Sch Med, Lab Med Invest, Unit 56, Sao Paulo, Brazil
hcfmusp.author.externalTOLEDO, M. S.:Univ Fed Sao Paulo, Lab Immunochem Glyconjugates, Sao Paulo, Brazil
hcfmusp.author.externalSTRAUS, A. H.:Univ Fed Sao Paulo, Lab Immunochem Glyconjugates, Sao Paulo, Brazil
hcfmusp.author.externalTAKAHASHI, H. K.:Univ Fed Sao Paulo, Lab Immunochem Glyconjugates, Sao Paulo, Brazil
hcfmusp.contributor.author-fmusphcDEWTON DE MORAES VASCONCELOS
hcfmusp.contributor.author-fmusphcALBERTO JOSE DA SILVA DUARTE
hcfmusp.contributor.author-fmusphcGIL BENARD
hcfmusp.description.beginpage395
hcfmusp.description.endpage396
hcfmusp.description.issuesuppl 1
hcfmusp.description.volume32
hcfmusp.origemWOS
hcfmusp.origem.wosWOS:000308728900756
hcfmusp.publisher.cityNEW YORK
hcfmusp.publisher.countryUSA
relation.isAuthorOfPublicationb0e53635-a63a-4e69-8f68-7f2e43a5f244
relation.isAuthorOfPublication8e4f2c15-06a5-444b-826d-84576403144e
relation.isAuthorOfPublication435ea94f-594d-4595-b5f5-1df649516e3c
relation.isAuthorOfPublication.latestForDiscovery435ea94f-594d-4595-b5f5-1df649516e3c
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Comunicações em Eventos - FM/MPT
Comunicações em Eventos - FM/MDT
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Comunicações em Eventos - LIM/03
Comunicações em Eventos - LIM/56