Evaluation of targets for Strongyloides genus specific molecular diagnosis in experimental strongyloidiasis

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorNASCIMENTO, Rafael C.
dc.contributor.authorMELO, Gessica B.
dc.contributor.authorFONSECA, Priscilla D. M.
dc.contributor.authorGRYSCHEK, Ronaldo C. B.
dc.contributor.authorPAULA, F. M.
dc.date.accessioned2021-12-16T14:25:05Z
dc.date.available2021-12-16T14:25:05Z
dc.date.issued2021
dc.description.abstractStrongyloides venezuelensis has been used in different experimental studies, such as those aimed at the evaluation of diagnostic techniques for human strongyloidiasis, mainly the molecular diagnosis. In this study, three regions (genus, 18S and 28S targets) of Strongyloides ribosomal DNA were evaluated for the molecular diagnosis of experimental strongyloidiasis. Rats were infected subcutaneously with 400 or 4000 S. venezuelensis infective larvae (400iL3 and 4000iL3), and kept for 35 days. Fecal samples were collected daily to count eggs per gram of feces (EPG) and to perform the polymerase chain reaction (PCR). Egg count started on the 5th day post-infection (pi) and ended on days 33 and 34 pi, in 400iL3 and 4000iL3 groups, respectively. Based in EPG, fecal samples were selected from days 2, 5, 8, 11, 15, 23 and 35 pi for DNA extraction; PCR (genus, 18S and 28S); and sequencing. The PCR-28S products showed higher values of identity (95-100%) in the database with the Strongyloides sequences. Therefore, it is possible to reinforce the application of PCR-28S in the diagnosis of experimental and human strongyloidiasis.eng
dc.description.indexMEDLINEeng
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao PauloFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [FAPESP 2016/06185-0, 2013/033040]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e TecnologicoConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)
dc.identifier.citationEXPERIMENTAL PARASITOLOGY, v.230, article ID 108157, 6p, 2021
dc.identifier.doi10.1016/j.exppara.2021.108157
dc.identifier.eissn1090-2449
dc.identifier.issn0014-4894
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/43868
dc.language.isoeng
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEeng
dc.relation.ispartofExperimental Parasitology
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright ACADEMIC PRESS INC ELSEVIER SCIENCEeng
dc.subjectExperimental strongyloidiasiseng
dc.subjectMolecular diagnosiseng
dc.subjectStrongyloides rDNAeng
dc.subject.otherreal-time pcreng
dc.subject.otherfecal sampleseng
dc.subject.othervenezuelensiseng
dc.subject.otherstercoraliseng
dc.subject.otherratseng
dc.subject.wosParasitologyeng
dc.titleEvaluation of targets for Strongyloides genus specific molecular diagnosis in experimental strongyloidiasiseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.citation.scopus0
hcfmusp.contributor.author-fmusphcRAFAEL CORREA DO NASCIMENTO
hcfmusp.contributor.author-fmusphcGESSICA BAPTISTA DE MELO
hcfmusp.contributor.author-fmusphcPRISCILLA DUARTE MARQUES FONSECA
hcfmusp.contributor.author-fmusphcRONALDO CESAR BORGES GRYSCHEK
hcfmusp.contributor.author-fmusphcFABIANA MARTINS DE PAULA
hcfmusp.description.articlenumber108157
hcfmusp.description.volume230
hcfmusp.origemWOS
hcfmusp.origem.pubmed34543651
hcfmusp.origem.scopus2-s2.0-85115098236
hcfmusp.origem.wosWOS:000717495500002
hcfmusp.publisher.citySAN DIEGOeng
hcfmusp.publisher.countryUSAeng
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