Leishmania enriettii visceralises in the trachea, lungs, and spleen of Cavia porcellus

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art_ALVES-SOBRINHO_Leishmania_enriettii_visceralises_in_the_trachea_lungs_and_2022.PDF (5.78 MB)
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0
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article
Data de publicação
2022
DOI
SciELO
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
FUNDACO OSWALDO CRUZ
Autores
ALVES-SOBRINHO, Edneia Venancio
PINHEIRO, Lucelia de Jesus
PARANAIBA, Larissa Ferreira
FONTES, Igor Campos
PARREIRAS, Patricia Martins
GONTIJO, Nelder Figueiredo
TAFURI, Wagner Luiz
SOARES, Rodrigo Pedro
Citação
MEMORIAS DO INSTITUTO OSWALDO CRUZ, v.117, article ID e220065, 10p, 2022
Projetos de Pesquisa
Unidades Organizacionais
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Resumo
BACKGROUND Leishmania (Mundinia) enriettii is a species commonly found in the guinea pig, Cavia porcellus. Although it is a dermotropic species, there is still an uncertainty regarding its ability to visceralise during Leishmania life cycle. OBJECTIVE Here, we investigated the ability of L. enriettii (strain L88) to visceralise in lungs, trachea, spleen, and liver of C. porcellus, its natural vertebrate host. METHODS Animals were infected sub-cutaneously in the nose and followed for 12 weeks using histological (hematoxilin-eosin) and molecular tools (polymerase chain reaction-restriction fragment length polymorphism - PCR-RFLP). To isolate parasite from C. porcellus, animals were experimentally infected for viscera removal and PCR typing targeting hsp70 gene. FINDINGS Histological analysis revealed intense and diffuse inflammation with the presence of amastigotes in the trachea, lung, and spleen up to 12 weeks post-infection (PI). Molecular analysis of paraffin-embedded tissues detected parasite DNA in the trachea and spleen between the 4th and 8th weeks PI. At the 12th PI, no parasite DNA was detected in any of the organs. To confirm that the spleen could serve as a temporary site for L. enriettii, we performed additional in vivo experiments. During 6th week PI, the parasite was isolated from the spleen confirming previous histopathological and PCR observations. MAIN CONCLUSION Leishmania enriettii (strain L88) was able to visceralise in the trachea, lung, and spleen of C. porcellus.
Palavras-chave
Leishmania enriettii, Cavia porcellus, subgenus Mundinia, visceralisation, histopathology, host-parasite interaction
URI
https://observatorio.fm.usp.br/handle/OPI/49369
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - LIM/50